Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effects of Soybean and DJI Chungkukjang Powder on Blood Glucose and Serum Lipid Reduction in db/db Mice

대두 및 DJI 청국장 분말이 db/db 마우스의 혈당과 혈청 지질 감소에 미치는 영향

  • Received : 2012.04.30
  • Accepted : 2012.05.30
  • Published : 2012.08.31
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Abstract

The hypoglycemic and hypolipidemic effects of autoclaved soy flour and DJI chungkukjang powder fermented using Bacillus subtilis DJI were investigated in type 2 diabetic animal models. After a 2-week adaptation period, the diabetic animal model db/db mice were divided into the diabetic control group (D-C group), a diabetic group fed with soybean (D-S group), and a diabetic group fed with DJI chungkukjang (D-CJ group). The body weight gain, food intake, water intake, liver, and adipose tissue weights were not significantly different between the experimental groups. The supplementation of DJI chungkukjang or autoclaved soy flour diet induced a marked reduction of fasting blood glucose, blood glycosylated hemoglobin levels, and glucose levels in the oral glucose tolerance test and AUC for glucose compared with the diabetic control group. However, DJI chungkukjang showed a much stronger antidiabetic effect than unfermented autoclaved soy flour. Serum insulin levels were the same among the groups. The supplementation of DJI chungkukjang or autoclaved soy flour diet also significantly lowered the serum triglyceride, total cholesterol, and LDL-cholesterol levels compared with the control diabetic group, while it elevated the HDL-cholesterol level in the serum. This data suggests that the dietary supplementation of autoclaved soy flour or DJI chungkukjang may be useful in the control of blood glucose in animals with type 2 diabetes.

본 연구는 대두와 B. subtilis DJI로 발효시켜 제조한 DJI 청국장 분말을 db/db 마우스에 장기간 급여하였을 경우 혈당 조절 및 지질대사 개선효과에 어느 것이 더 크게 영향을 미치는지 알아보기 위하여 실시하였다. 실험군은 당뇨병 대조군(D-C), 대두 분말 급여군(D-S군) 및 DJI 청국장 분말 급여군(D-CJ군)으로 3군으로 나누어 6주간 실시하였다. 체중증가량, 식이섭취량, 수분섭취량 및 간과 지방조직의 무게는 실험군 간에 차이가 없었다. 전혈의 공복 혈당 변화는 4주째부터 D-CJ군이 당뇨병 대조군인 D-C군에 비하여 유의하게 감소되었고, 6주째에는 D-S군과 D-CJ군이 D-C군에 비하여 각각 약 21.39%와 23.82%씩 유의하게 감소되었다. 혈청 중 인슐린 농도는 실험군 간에 차이가 나타나지 않았다. 당화 헤모글로빈의 함량은 D-C군에 비하여 D-CJ군이 유의하게 감소되었다. 내당능 검사에서는 대두 혹은 DJI 청국장 분말 급여로 90분부터 당부하가 유의적으로 감소하는 경향이었다. 혈당반응면적(AUC)은 DJI 청국장 분말 급여한 D-CJ군이 D-C군에 비하여 유의하게 낮았다. 혈청 중 총콜레스테롤 함량은 D-S군과 D-CJ군 모두 D-C군에 비하여 유의하게 감소되었으나, 중성지방과 LDL-콜레스테롤 함량은 DJI 청국장 분말을 급여한 D-CJ군만이 D-C군에 비하여 유의하게 감소되었다. 혈청 중 HDL-콜레스테롤 함량은 D-CJ군이 D-C군에 비하여 유의하게 증가되었다. 이상의 결과 대두 혹은 DJI 청국장 분말의 급여는 db/db 마우스의 혈당, 당화 헤모글로빈 및 혈청 지질 농도를 감소시키는데 긍정적으로 작용하는 것으로 보이나, 대두 분말보다는 DJI 청국장 분말 급여 시 혈당 조절 및 지질대사 개선효과가 더 우수한 것으로 나타났다.

Keywords

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