YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Increased Expression of Type I Collagen in AF-343 Treated Human Skin Fibroblasts

섬유아세포에서 민들레 추출물 함유제(AF-343)에 의한 제 1형 교원질 발현 증가

  • Cho, Soo-Muk (Functional Food & Nutrition Division, Department of Agrofood Resources, NAAS, RDA) ;
  • Kim, Jae-Hyun (Functional Food & Nutrition Division, Department of Agrofood Resources, NAAS, RDA) ;
  • Kim, Jong-Keun (Sung Kyun Biotech Co., Ltd.) ;
  • Park, Ki-Moon (Department of Food Science and Biotechnology, Sungkyunkwan University) ;
  • Cho, Ho-Chan (Departments of Endocrinology, Internal Medicine, Keimyung University College of Medicine) ;
  • Kim, In-Su (Departments of Dermatology, Chung-Ang University College of Medicine) ;
  • Kim, Beom-Joon (Departments of Dermatology, Chung-Ang University College of Medicine) ;
  • Chun, Young-Jin (Chung-Ang University College of Pharmacy) ;
  • Cho, Jae-We (Dermatology, Keimyung University College of Medicine)
  • 조수묵 (국립농업과학원 농식품자원부 기능성식품과) ;
  • 김재현 (국립농업과학원 농식품자원부 기능성식품과) ;
  • 김종근 ((주)성균바이오텍) ;
  • 박기문 (성균관대학교 식품생명과학부) ;
  • 조호찬 (계명대학교 의과대학 내과학교실) ;
  • 김인수 (중앙대학교 의과대학 피부과학교실) ;
  • 김범준 (중앙대학교 의과대학 피부과학교실) ;
  • 천영진 (중앙대학교 약학대학) ;
  • 조재위 (계명대학교 의과대학 피부과학교실)
  • Received : 2011.10.24
  • Accepted : 2011.12.26
  • Published : 2012.02.29
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Abstract

We previously reported that the extract of Taraxacum platycarpum (AF-343) had several biological properties such as skin hydration and anti-inflammatory effects, thereby AF-343 be a promising anti-atopic dermatitis agent. However, few studies have been conducted to evaluate its effect on modulation of extracellular matrix proteins in human skin fibroblasts. The purpose of this study was to investigate the expressions of type I collagen, MMP-1, Smad2/3, and TIMP-1 proteins in AF-343-treated human skin fibroblasts. Human skin fibroblasts were treated by various concentrations of AF-343 (0~2 mg/ml). The expressions of type I collagen, matrix metalloproteinase-1 (MMP-1), Smad2/3, and TIMP-1 proteins were analyzed by Western blot analysis. In addition, level of type I collagen mRNA was analyzed by CAT assay. Expression of type I collagen protein was increased in AF-343-treated human skin fibroblasts by dose and time-dependent manners. Consistent with this result, the expressions of phospho-Smad2/3 in skin fibroblasts were increased and MMP-1 expression was decreased by AF-343 treatment. TIMP-1 expression was not significantly changed in AF-343 treated skin fibroblasts. Extract of Taraxacum platycarpum (AF-343)-induced up-regulation of type I collagen expression was through increased expression of phospho-Smad2/3. These results were occurred combined with down-regulation of MMP-1 in skin fibroblasts. Taken together, this study indicated that AF-343 has property of the modulation of ECM in tissue as well as skin hydration and anti-inflammation.

Keywords

References

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