Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Analysis of Diarrhetic Shellfish Poisoning Toxins by Liquid Chromatography-electrospray Ionization Mass Spectrometry

LC-MS/MS를 이용한 설사성 패류독소 함량 조사

  • Kim, Su-Un (Seoul Metropolitan Goverment Research Institute of Health and Environment, Fisheries Products Inspection Team) ;
  • Yuk, Dong-Hyun (Seoul Metropolitan Goverment Research Institute of Health and Environment, Fisheries Products Inspection Team) ;
  • Park, Young-Ae (Seoul Metropolitan Goverment Research Institute of Health and Environment, Fisheries Products Inspection Team) ;
  • Kim, Jin-Ah (Seoul Metropolitan Goverment Research Institute of Health and Environment, Fisheries Products Inspection Team) ;
  • Park, Ae-Sook (Seoul Metropolitan Goverment Research Institute of Health and Environment, Fisheries Products Inspection Team) ;
  • Kim, Yun-Chun (Seoul Metropolitan Goverment Research Institute of Health and Environment, Fisheries Products Inspection Team)
  • 김수언 (서울시보건환경연구원 수산물검사팀) ;
  • 육동현 (서울시보건환경연구원 수산물검사팀) ;
  • 박영애 (서울시보건환경연구원 수산물검사팀) ;
  • 김진아 (서울시보건환경연구원 수산물검사팀) ;
  • 박애숙 (서울시보건환경연구원 수산물검사팀) ;
  • 김연천 (서울시보건환경연구원 수산물검사팀)
  • Received : 2012.03.02
  • Accepted : 2012.05.02
  • Published : 2012.08.31
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Abstract

Diarrhetic shellfish poisoning toxins were investigated by liquid chromatography-electrospray ionization mass spectrometry (LC-MS/MS). Okadaic acid (OA), Dinophysistoxin-1 (DTX1), Pectonotoxin2, (PTX2) and Yessotoxin (YTX) in bivalves were quantified. OA were found in four samples; mussel Mytilus edulis (0.001 ${\mu}g/g$), Oyster Crassostrea gigas (0.004 and 0.001 ${\mu}g/g$) and manila clam Ruditapes philippinarum (0.001 ${\mu}g/g$). DTX1, PTX2, and YTX were not detected from all of the samples examined.

LC/MS/MS로 패류 114건에서 설사성패독인 OA, DTX1, PTX2, YTX을 정량한 결과 110건(96.5%)에서는 설사성패독이 불검출이었으며, 굴 2건(0.004, 0.001 ${\mu}g/g$), 진주담치 1건(0.001 ${\mu}g/g$), 바지락 1건(0.001 ${\mu}g/g$)에서 OA(Okadaic acid)가 검출되어 3.5%의 검 출률을 나타내었으며 동일 시료를 마우스시험법으로 마비성 패독을 실험한 결과 설사성 패독과의 유의성은 보이지 않았다.

Keywords

References

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