Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Evaluation of the Bactericidal Activity of Electrolyzed Water against Salmonella Typhimurium and Staphylococcus aureus on Perilla Leaves

전해수를 이용한 들깻잎 중 Salmonella Typhimurium과 Staphylococcus aureus의 저감화 효과 분석

  • 김세리 (농촌진흥청 국립농업과학원 농산물안전성부) ;
  • 류경열 (농촌진흥청 연구정책국 평가관리과) ;
  • 이명희 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 정찬식 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 윤요한 (숙명여자대학교 생활과학부 식품영양학과) ;
  • 심현보 (경상대학교 응용생명과학부) ;
  • 김주희 (농촌진흥청 국립농업과학원 농산물안전성부) ;
  • 김병석 (농촌진흥청 국립농업과학원 농산물안전성부) ;
  • 유순영 (서울지방식품의약품안전청) ;
  • 김두호 (농촌진흥청 국립농업과학원 농산물안전성부) ;
  • 윤종철 (농촌진흥청 국립농업과학원 농산물안전성부) ;
  • 정덕화 (경상대학교 응용생명과학부)
  • Received : 2012.03.28
  • Accepted : 2012.06.02
  • Published : 2012.08.31
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Abstract

This study was conducted to evaluate the bactericidal activity of weakly alkalic electrolyzed water (WEW) against Salmonella Typhimurium and Staphylococcus aureus on perilla leaves. The influences of organic matter, inform of bovine serum, and the ratio of WEW to perilla leaves on bactericidal activity of WEW were also examined. Treatment of these organisms with 25, 50, 75, and 100 ppm WEW was performed for 1 min, 3 min and 5 min, respectively. Higher bactericidal activity was observed after a treatment with 100 ppm WEW compared to a treatment with 25 ppm WEW by 0.7 $log_{10}CFU/g$. The bactericidal activity of WEW also decreased with increasing bovine serum concentration. At the ratio of 10:1 (WEW: perilla leave), levels of Salmonella Typhimurium and Staphylococcus aureus were only reduced by 0.57 and 0.79 $log_{10}CFU/g$, respectively. It is suggested that the removal of organic debris prior to application of sanitizers and treatment above the ratio of 25:1 (WEW: perilla leave) is needed in order to improve WEW activity.

본 연구는 들깻잎에 존재하는 Salmonella Typhimurium, Staphylococcus aureus에 대한 전해수의 저감효과를 분석하고 전해수로의 유기물의 유입과 들깻잎과 전해수의 비율이 전해수의 효과에 미치는 영향을 분석하고자 수행하였다. 들깻잎에 S. Typhimurium과 S. aureus을 7.0 $log_{10}CFU/g$ 정도로 접종 한 후 25, 50, 75, 100 ppm에서 1, 3, 5분 동안 각각 처리하여 시간과 농도에 따른 전해수 효과를 분석하였다. 또한 유기물이 전해수의 효과에 미치는 영향을 분석하기 위해 전해수에 첨가한 유기물 농도는 150, 300, 450, 600 ${\mu}g/L$ 수준이었고, 들깻잎과 전해수의 처리비율은 1:10, 1:25, 1:50이었다. 그 결과, S. Typhimurium과 S. aureus 모두 유효 염소 농도가 증가할수록 미생물 저감효과도 높아지는 경향을 보였으며(p<0.05) 두 균주 모두 100 ppm 처리에서 25 ppm 처리에서 보다 전해수의 저감효과가 약 0.7 $log_{10}CFU/g$ 정도 높게 나타났다(p<0.05). 하지만 처리 시간에 따른 효과는 없었다(p>0.05). 또한 전해수 중 유기물 함량이 증가할수록 S. Typhimurium과 S. aureus의 제어효과는 감소하였다(p<0.05). 유기물 600 ${\mu}g/L$ 첨가시 유기물 첨가를 하지 않았을 때에 비하여 S. Typhimurium은 1.10 $log_{10}CFU/g$, S. aureus는 0.8 $log_{10}CFU/g$의 전해수 효과가 감소하였다. 들깻잎과 전해수의 처리비율은 1:10으로 처리하였을 때 미생물 저감효과는 S. Typhimurium 0.57 $log_{10}CFU/g$, S. aureus 0.79 $log_{10}CFU/g$로 전해수에 의한 미생물저감 효과를 기대하기 어려웠다. 따라서 들깻잎에서 S. Typhimurium과 S. aureus의 효과적인 제어를 위해서는 전해수 처리전에 물 세척을 통하여 흙과 먼지를 제거하고 전해수 농도 100 ppm에서 1분 이상 처리해야한다. 또한 들깻잎과 전해수 비율은 1:25 이상 유지하는 것이 바람직할 것으로 사료된다.

Keywords

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