DOI QR코드

DOI QR Code

Effect of Vitamin E Supplementation on Hematological and Plasma Biochemical Parameters during Long Term Exposure of Arsenic in Goats

  • Das, Tapan Kumar (Dairy Cattle Nutrition Division, National Dairy Research Institute) ;
  • Mani, Veena (Dairy Cattle Nutrition Division, National Dairy Research Institute) ;
  • Kaur, Harjit (Dairy Cattle Nutrition Division, National Dairy Research Institute) ;
  • Kewalramani, Neelam (Dairy Cattle Nutrition Division, National Dairy Research Institute) ;
  • Agarwal, Anjali (Dairy Cattle Nutrition Division, National Dairy Research Institute)
  • 투고 : 2012.01.05
  • 심사 : 2012.03.23
  • 발행 : 2012.09.01

초록

The present investigation was designed to determine whether supplementation of different level of vitamin E for 12 months to arsenic exposed goats (50 ppm as sodium arsenite) affords protection against the blood hemato-biochemical parameters caused by the metalloid. A total of 24 crossbred (Alpine${\times}$Beetal) lactating goats were assigned randomly into 4 equal groups (control, $T_1$, $T_2$ and $T_3$) of 6 in each, on the basis of average body weight ($36.10{\pm}0.11$ kg) and milk yield ($1.61{\pm}0.04$ kg/d). The animals in $T_1$, $T_2$ and $T_3$ were given 50 ppm arsenic, while in $T_2$ and $T_3$, additionally; vitamin E at the rate of 100 IU and 150 IU/kg dry matter (DM) respectively was additionally supplemented for the period of 12 months. Hemoglobin (Hb), total leukocyte (TLC) and blood lymphocyte % were decreased (p<0.05) in arsenic fed groups and vitamin E supplementation in the experimental group showed a protective potential. Significant increases (p<0.05) in aspertate transaminase (AST) and alanine transaminase (ALT) activities among arsenic supplemented groups were recorded, however vitamin E supplementation at higher doses showed a protective effect (p<0.05) against AST but in the case of ALT no ameliorating effect was found in either of the doses. Plasma total protein was decreased (p>0.05) but creatinine level was periodically increased in all As supplemented groups and vitamin E supplementation did not produce any protective effect. It can be concluded that arsenic exposure resulted in varying degree of changes in hemato-biochemical parameters and activities of antioxidant enzymes in goats but concomitant treatment with Vitamin E is partially helpful in reducing the burden of arsenic induced effect.

키워드

참고문헌

  1. Abbas, S. F. 2002. Effect of vitamin E and selenium injections on lamb viability, growth performance and some blood serum constituents in Saibi lambs. Assiut Vet. Med. J. 47:129-136.
  2. AOAC. 1990. Official methods of analysis. Association of Official Analytical Chemists, 15th Ed. Washington, DC, USA.
  3. Biswas, U., S. Sarkar, M. K. Bhowmik and S. Roy. 1998. Clinicopathological profile of induced chronic arsenic toxicity in goats. Indian J. Anim. Sci. 68:320-323.
  4. Biswas, U., S. Sarkar, M. K. Bhowmik, S. K. Samanta and S. Biswas. 2000. Chronic toxicity of arsenic in goats: clinicobiochemical changes, pathomorphology and tissue residues. Small Rumin. Res. 38:229-235. https://doi.org/10.1016/S0921-4488(00)00162-0
  5. Bonses, R. W. and H. H. Taussky. 1945. On the colorimetric determination of creatinine by the Jaffe reaction. J. Biol. Chem. 158:581-591.
  6. Drabkin, D. L. 1944. Photometry and spectrometry: Medical physics, Vol. 1, Year book medical publishers. Inc., Chicago, USA.
  7. Garcia-Shavez, E., I. Jimenez, B. Segura and L. M. D. Razo. 2006. Lipid peroxidative damage and distribution of inorganic and its metabolite in the rat nervous system after arsenite exposure: influence of alpha tocopherol. Neurotoxicology 27:1024-1031. https://doi.org/10.1016/j.neuro.2006.05.001
  8. Hardman, G. and L. E. Limbird. 1996. Goodman and Gilman's The Pharmacological Basis of Therapeutics. 9th edn, McGraw-Hill Companies, New York, pp. 633-634.
  9. Healy, S. M. and A. V. Aposhian. 1999. Diversity of inorganic arsenite transformation. Biol. Trace Elem. Res. 60:249-266.
  10. Hughes, M. F. 2002. Arsenic toxicity and potential mechanisms of action. Toxicol. Lett. 133:1-16. https://doi.org/10.1016/S0378-4274(02)00084-X
  11. Ianchev, I. 2001. Influence of some geo-chemical ecological factors on some blood hematological characteristics in sheep from Chiprovitei. Zhivotnovu d.-Nauki. 38:41-43.
  12. Kaneko, J. J. 1980. Clinical biochemistry of domestic animals. 3rd Edn. Academic press, NY, USA.
  13. Koller, A. 1984. Proteins. In: Clinical Chemistry: theory, analysis and correlation (Ed. L. A. Kaplan and A. J. Pesce). C.V.Mosby, Toranto, Canada. pp. 1268-1327.
  14. Liu, S. X., M. Athar, I. Lippai, C. Waldren and T. K. Hei. 2001. Induction of oxy-radicals by arsenic: implication for mechanism of genotoxicity. Proc. Natl Acad. Sci. 98:1643-1648. https://doi.org/10.1073/pnas.98.4.1643
  15. Manning, P. J., W. H. F. Suther and R. J. Walker. 2004. Effect of high dose vitamin E on insulin resistance and associated parameters in overweight subjects. Diabetes Care 27:2166. https://doi.org/10.2337/diacare.27.9.2166
  16. Mishra, C. S., V. Mani and H. Kaur. 2005. Effect of arsenic on immunity, oxidative enzyme and various haematological parameters in crossbred calves. Asian-Aust. J. Anim. Sci. 18:497-501. https://doi.org/10.5713/ajas.2005.497
  17. Morand-Fehr, P. 1981. In "Goat Production" (Ed. G. Gall). pp. 193-232. Academic Press, NY, USA.
  18. Nandi, D., R. C. Patra and D. Swarup. 2005. Effect of cysteine, methionine, ascorbic acid and thiamine on arsenic induced oxidative stress and biochemical alterations in rats. Toxicology 211:26-35. https://doi.org/10.1016/j.tox.2005.02.013
  19. Nandi, D., R. C. Patra and D. Swarup. 2006. Oxidative stress indices and plasma biochemical parameters during oral exposure to arsenic in rats. Food Chem. Toxicol. 44:1579-1584. https://doi.org/10.1016/j.fct.2006.04.013
  20. NRC. 1981. Nutrient requirements of goats: angora, dairy and meat goats in temperate and tropical countries. National Academy Press, Washington DC, USA.
  21. Pandey, P. K., M. Roy and S. Roy. 2005. Clinico-haematological and biochemical alterations in acute arsenic toxicity in goats. Indian J. Vet. Med. 25:57-60.
  22. Patel, A. K., N. Kewalramani, V. Mani and H. Kaur. 2009. Effect of vitamin E supplementation in growing kids fed on arsenic containing diet. Indian J. Anim. Nutr. 26:9-16.
  23. Rabbani, G. H., S. K. Saha, A. Mastura and M. Farzana. 2003. Antioxidants in detoxification of arsenic induced oxidative injury in rabbits. J. Environ. Sci. Health A 38:273-287. https://doi.org/10.1081/ESE-120016894
  24. Ramanathan, K., B. S. Balakumar and C. Paneerselvam. 2002. Effects of ascorbic acid and $\alpha$-tocopherol on arsenic-induced oxidative stress. Hum. Exp. Toxicol. 21:675-680. https://doi.org/10.1191/0960327102ht307oa
  25. Rana, T., A. K. Bera, S. Das, D. Bhattacharya, S. Bandyopadhyay, D. Pan and S. K. Das. 2010. Effect of chronic intake of arsenic-contaminated water on blood oxidative stress indices in cattle in an arsenic-affected zone. Ecotoxicol. Environ. Saf. 73:1327-1332. https://doi.org/10.1016/j.ecoenv.2010.06.002
  26. Rana, T., S. Sarkar, T. Mandal and S. Batabyal. 2008. Hemato-biochemical profiles of affected cattle at arsenic prone zone in Haringhata block of Nadia District of West Bengal in India. Inter. J. Hematol. 4:2.
  27. Reitman, S. and S. Frankel. 1957. Colorimetric method for the determination of SGOT and SGPT. Am. J. Clin. Pathol. 33:97.
  28. Santra, A., J. Das Gupta, B. K. De, B. Roy and D. N. Guha Mazumer. 1999. Hepatic manifestations in chronic arsenic toxicity. Indian J. Gastroenterol. 18:152-155.
  29. Uthus, E. O. 2001. High dietary arsenic exacerbates copper deprivation in rats. J. Trace Elem. Exp. Med. 14:43-55. https://doi.org/10.1002/1520-670X(2001)14:1<43::AID-JTRA1007>3.0.CO;2-#
  30. Vaswani, S., V. Mani, N. Kewalramani and H. Kaur. 2010. Mitigation of adverse effects of arsenic by supplementing vitamin E in crossbred kids maintained at low protein diet. Indian J. Anim. Nutr. 27:347-354.
  31. Wang, L., Z. R. Xu, X. Y. Jia and X. Y. Han. 2006. Effects of dietary arsenic levels on serum parameters and trace mineral retentions in growing and finishing pigs. Biol. Trace Elem. Res. 113:155-164. https://doi.org/10.1385/BTER:113:2:155
  32. Woods, J. S. and B. A. Fowler. 1986. Alteration of hepatocellular structure and function by thallium chloride: Ultra structural, morphometric, and biochemical studies. Toxicol. Applied Pharmacol. 83:218-229. https://doi.org/10.1016/0041-008X(86)90299-1

피인용 문헌

  1. Rising level of arsenic in water and fodder: a growing threat to livestock and human populations in Pakistan pp.1556-9551, 2018, https://doi.org/10.1080/15569543.2017.1348360
  2. A review on hemato-biochemical, accumulation and patho-morphological responses of arsenic toxicity in ruminants pp.1556-9551, 2018, https://doi.org/10.1080/15569543.2018.1442347
  3. Sodium arsenite toxicity on hematology indices and reproductive parameters in Teddy goat bucks and their amelioration with vitamin C vol.27, pp.13, 2012, https://doi.org/10.1007/s11356-020-08049-z