Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
권호 표지
DOI QR코드

DOI QR Code

Isolation and Identification of Lipids from the Fruits of Acanthopanax sessiliflorus

오가피(Acanthopanax sessiliflorus Seeman) 열매로부터 지질 화합물의 분리 및 동정

  • Kim, Su-Yeon (Graduate School of Biotechnology & Department of Oriental Medicinal Materials and Processing, Kyung Hee University) ;
  • Lee, Dae-Young (Graduate School of Biotechnology & Department of Oriental Medicinal Materials and Processing, Kyung Hee University) ;
  • Seo, Kyeong-Hwa (Graduate School of Biotechnology & Department of Oriental Medicinal Materials and Processing, Kyung Hee University) ;
  • Rho, Young-Deok (Graduate School of Biotechnology & Department of Oriental Medicinal Materials and Processing, Kyung Hee University) ;
  • Kim, Gye-Won (Brewing Research Center, Hankyung National University) ;
  • Cheoi, Dae-Sung (Jeongseon Agricultural Extension Center) ;
  • Baek, Nam-In (Graduate School of Biotechnology & Department of Oriental Medicinal Materials and Processing, Kyung Hee University)
  • Received : 2012.03.28
  • Accepted : 2012.05.08
  • Published : 2012.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Acanthopanax sessiliflorus Seeman fruits (Araliaceae) were extracted at room temperature with 70% aqueous ethanol (EtOH). The concentrated extract was partitioned with ethyl acetate (EtOAc), n-butyl alcohol, and $H_2O$, successively. From the EtOAc fraction, four compounds were isolated through the repeated silica gel and octadecyl silica gel (ODS) column chromatographies. According to the results of physico-chemical and spectroscopic data including NMR, IR, and gas chromatography/mass spectrometer, the chemical structures of the compounds were determined as stigmasterol (1), linoleic acid (2), ${\beta}$-sitosterol (3), and stigmast-5-en-$3{\beta}$,$7{\beta}$-diol (4).

오가피(Acanthopanax sessiliflorus Seeman) 열매 10.0 kg을 실온에서 70% EtOH 수용액으로 추출하고 이 추출물을 ethyl acetate (EtOAC) 분획, n-butyl alcohol 분획, $H_2O$ 분획으로 나누었다. EtOAc 분획에 대하여 silica gel 및 octadecyl silica gel chromatography column chromatography를 반복 실시하여 4종의 화합물을 분리, 정제하였다. NMR, IR, EI/MS, 및 GC/MS 등을 통하여 화합물 1(stigmasterol), 화합물 2(linoleic acid), 화합물 3(${\beta}$-sitosterol), 화합물 4(stigmast-5-en-$3{\beta}$,$7{\beta}$-diol)의 구조를 결정하였다.

Keywords

References

  1. Ahn JK, Park SY, Lee WY, and Lee JJ (2005) Effects of growth regulators on adventitious root growth and eleutherosides and chlorogenic acid accumulation in air lift bioreactor culture of Eleutherococcus koreaum. Korean J Plant Biotechnol 32, 57-61. https://doi.org/10.5010/JPB.2005.32.1.057
  2. Akino N (1975) Antioxidants from Rape (Brassica campestris var. japonica Hara) oil cake. Nat Prod RES 18, 231-9.
  3. Brekhman II and Dardmov IV (1969) New substances of plant origin which increase nonspecific resistance. Annu Rev Pharmacol 9, 419-30. https://doi.org/10.1146/annurev.pa.09.040169.002223
  4. Choi JM, Kim KY, Lee SH, and Ahn JB (2011) Functional properties of water extracts from different parts of Acanthopanax sessiliflorus. Food Engineering Progress 15, 130-5.
  5. Ghorab AH, Mahgoub MH, and Bekheta M (2006) Effect of some bioregulators on the chemical composition of essential oil and its antioxidant activity of Egyptian carnation (Dianthus caryophyllus L.). J Essent Oil-Bearing Plants 9, 214-22. https://doi.org/10.1080/0972060X.2006.10643494
  6. Glisic SB, Misic DR, Stamenic MD, Zizovic IT, Asanin RM, and Skala DU (2007) Supercritical carbon dioxide extraction of carrot fruit essential oil: chemical composition and antimicrobial activity. Food Chem 105, 346- 52. https://doi.org/10.1016/j.foodchem.2006.11.062
  7. Habib MR, Nikkon F, Rahman M, Haque ME, and Karim MR (2007) Isolation of stigmasterol and beta-sitosterol from methanolic extract of root bark of Calotropis gigantea (Linn). Pakistan J Biol Sci 10, 4174-6. https://doi.org/10.3923/pjbs.2007.4174.4176
  8. Hyun JW, Yang YM, Sung MS, Chung HS, Paik WH, Kang SS et al. (1996) The Cytotoxic activity of sterol derivatives from Pulsatilla chinensis regal. J Korean Cancer 28, 145-50.
  9. Jeong SC, Jeong YT, Yang BK, and Song CH (2006) Chemical characteristics and immuno-stimulating properties of biopolymers extracted from Acanthopanax sessiliflorus. Biochem Molecular Biol 39, 84-90. https://doi.org/10.5483/BMBRep.2006.39.1.084
  10. Jing JL, Lee SH, Lee YY, Kim JM, Heo JE, and Yun-Choi HS (2004) Platelet anti-aggregating triterpenoids from the leaves of Acanthopanax senticosus and the fruits of A. sessiliflorus. Planta Medica 70, 564-6. https://doi.org/10.1055/s-2004-827159
  11. Kwak HY, Lee SJ, Lee DY, Jung LK, Bae NH, Hong SY et al. (2008) Cytotoxic and anti-inflammatory activities of lipids from the Nuruk (Rhizopus oryzae KSD-815). J Korean Soc Appl Biol Chem 51, 142-7.
  12. Lee DY, Lee SJ, Kwak HY, Jung LK, Heo JE, Hong SY et al. (2009) Sterols Isolated from Nuruk (Rhizopus oryzae KSD-815) inhibit the migration of cancer cells. J Microbiol Biotechnol 19, 1328-32.
  13. Lee SH, Kim BK, Cho SH, and Shin KH (2002) Phyrochemical constituents from the fruits of Acanthopanax sessiliflorus. Arch Pharm Res 25, 280- 4. https://doi.org/10.1007/BF02976626
  14. Lee SH, Lee YS, Jung SH, Ji J, Shin KH, Kim BK et al. (2003) Antitumor and immunostimulating activities of Acanthopanax sessiliflorus fruits. Nat Prod Sci 9, 112-6.
  15. Lee WT (1979) Distribution of Acanthopanax plants in Korea. Korean J Pharmacol 10, 103-7.
  16. Lee WT (1996) In Lineamenta Florae Korea (vol. 770). Academy CO, Korea.
  17. Ryoo HS, Park SY, Chang SY, and Yook CS (2003) Triterpene components from the leaves of Acanthopanax sessiliflorus Seem. Korean J Pharmacogn 34, 269-73.
  18. Song MS, Lee YW, and Kim JD (2003) Extraction of Acanthopanax senticosus by supercitical fluid. Hwahak Konghak 41, 207-12.

Cited by

  1. New Polyprenylated Phloroglucinol and Other Compounds Isolated from the Fruits of Clusia nemorosa (Clusiaceae) vol.20, pp.8, 2015, https://doi.org/10.3390/molecules200814326
  2. 차광과 적심높이에 따른 흰털오갈피나무와 섬오갈피나무의 생육과 Acanthoside-D 함량 변화 vol.21, pp.1, 2012, https://doi.org/10.7783/kjmcs.2013.21.1.39
  3. Inhibitory Effects of seco-Triterpenoids from Acanthopanax sessiliflorus Fruits on HUVEC Invasion and ACE Activity vol.10, pp.9, 2012, https://doi.org/10.1177/1934578x1501000907