Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
권호 표지

Effects of Open versus Laparoscopic Cholecystectomy on Oxidative Stress in Dogs

개에서 산화 스트레스 상태에 대한 일반 개복술 대 복강경 수술을 통한 담낭절제술의 영향

  • Lee, Jae-Yeon (Department of Veterinary Surgery, College of Veterinary Medicine.Research Institute of Veterinary Medicine, Chungnam National University) ;
  • Jeong, Seong-Mok (Department of Veterinary Surgery, College of Veterinary Medicine.Research Institute of Veterinary Medicine, Chungnam National University) ;
  • Kim, Myung-Cheol (Department of Veterinary Surgery, College of Veterinary Medicine.Research Institute of Veterinary Medicine, Chungnam National University)
  • 심사 : 2012.08.20
  • 발행 : 2012.08.30
Download PDF
⟨ Previous Next ⟩

초록

In the present study, we investigated and compared the oxidant-antioxidant status of dogs undergoing open and laparoscopic cholecystectomy. Ten male beagle dogs, 4-6 years old, weighing 7-12 kg were used. The animals were randomly assigned to one of two groups according to the type of surgical procedure; open (group 1, n = 5) or laparoscopic cholecystectomy (group 2, n = 5). Heart rate, mean arterial pressure, end-tidal carbon dioxide, peripheral oxygen saturation, and respiratory rates were measured. Plasma total oxidant status (TOS) and total antioxidant status (TAS) levels for the evaluation of oxidative stress were measured. The ratio of the TOS to the TAS gave oxidative stress index (OSI), which is an indicator of the degree of oxidative stress. TOS, OSI and TAS levels were significantly changed after surgery in comparison to levels of before anesthesia. There were no significant different between groups. In the present study, we showed that oxidative stress parameters were found similar in dogs underwent laparoscopic or open cholecystectomy.

본 연구에서는 개에서 일반 개복술을 통한 담낭절제술과 복강경을 이용한 담낭절제술에서 생체 내 산화-항산화 상태에 미치는 영향을 비교 연구하였다. 10 마리의 비글견을 사용하였고 일반 개복술을 통한 담낭절제술군 (group 1, n = 5)과 복강경을 이용한 담낭절제술군 (group 2, n = 5)으로 분류하였다. 심박수, 혈압, 호기말 이산화탄소 분압, 말초 산소포화도, 호흡수를 측정하였다. 산화 스트레스 평가를 위한 총 산화 상태 (total oxidant status, TOS) 및 총 항산화 상태 (total antioxidant status, TAS) 수준이 측정되었다. TAS에 대한 TOS의 비율로 산화 스트레스 지수 (OSI)를 평가하였다. 수술 후에 두 군 모두 TOS, OSI, TAS의 유의성 있는 변화가 관찰되었으나 군 간의 유의성은 없었다. 본 실험을 통해 개에서 일반 개복술을 통한 담낭절제술과 복강경을 이용한 담낭절제술에서 생체 내 산화스트레스 상태에 미치는 영향은 비슷하게 나타났다.

키워드

참고문헌

  1. Erel O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem 2004; 37: 277-285. https://doi.org/10.1016/j.clinbiochem.2003.11.015
  2. Glantzounis GK, Tselepis AD, Tambaki AP, Trikalinos TA, Manataki AD, Galaris DA, Tsimoyiannis EC, Kappas AM. Laparoscopic surgery-induced changes in oxidative stress markers in human plasma. Surg Endosc 2001; 15: 1315-1319. https://doi.org/10.1007/s00464-001-0034-2
  3. Kama NA, Kologlu M, Doganay M, Reis E, Atli M, Dolapci M. A risk score for conversion from laparoscopic to open cholecystectomy. Am J Surg 2001; 181: 520-525. https://doi.org/10.1016/S0002-9610(01)00633-X
  4. Koksal H and Kurban S. Total oxidant status, total antioxidant status, and paraoxonase and arylesterase activities during laparoscopic cholecystectomy. Clinics 2010; 65: 285-290. https://doi.org/10.1590/S1807-59322010000300008
  5. Liu PT, Symons AM, Howarth JA, Boulter PS, Parke DV. Studies in surgical trauma: oxidative stress in ischaemiareperfusion of rat liver. Clin Sci 1994; 86 : 453-460.
  6. Mishra V. Oxidative stress and role of antioxidant supplementation in critical illness. Clin Lab 2007; 53: 199-209.
  7. Sare M, Yilmaz I, Hamamci D, Birincioglu M, Ozmen M, Yesilada O. The effect of carbon dioxide pneumoperitoneum on free radicals. Surg Endosc 2000; 14: 649-652. https://doi.org/10.1007/s004640000168
  8. Schirmer BD, Edge SB, Dix J, Hyser MJ, Hanks JB, Jones RS. Laparoscopic cholecystectomy. Treatment of choice for symptomatic cholelithiasis. Ann Surg 1991; 213: 665-676. https://doi.org/10.1097/00000658-199106000-00018
  9. Wellwood J, Sculpher MJ, Stoker D, Nicholls GJ, Geddes C, Whitehead A, Singh R, Spiegelhalter D. Randomised controlled trial of laparoscopic versus open mesh repair for inguinal hernia: outcome and cost. BMJ 1998; 317: 103-110. https://doi.org/10.1136/bmj.317.7151.103
  10. Zacks SL, Sandler RS, Rutledge R, Brown RS Jr. A population-based cohort study comparing laparoscopic cholecystectomy and open cholecystectomy. Am J Gastroenterol 2002; 97: 334-340. https://doi.org/10.1111/j.1572-0241.2002.05466.x
  11. Zulfikaroglu B, Koc M, Soran A, Isman FK, Cinel I. Evaluation of oxidative stress in laparoscopic cholecystectomy. Surg Today 2002; 32: 869-874. https://doi.org/10.1007/s005950200169