Asian Pacific Journal of Cancer Prevention

  • 제13권6호
  • /
  • Pages.2813-2818
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  • 2012
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  • 1513-7368(pISSN)
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  • 2476-762X(eISSN)
아시아태평양암예방학회 (Asian Pacific Journal of Cancer Prevention)
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Establishment and Characterization of MTDH Knockdown by Artificial Micro RNA Interference - Functions as a Potential Tumor Suppressor in Breast Cancer

  • Wang, Song (Department of Oncosurgery, Affiliated Nanhua Hospital) ;
  • Shu, Jie-Zhi (Department of Oncosurgery, Affiliated Nanhua Hospital) ;
  • Cai, Yi (Department of Oncosurgery, Affiliated Nanhua Hospital) ;
  • Bao, Zheng (Department of Oncosurgery, Affiliated Nanhua Hospital) ;
  • Liang, Qing-Mo (Department of Oncosurgery, Affiliated Nanhua Hospital)
  • 발행 : 2012.06.30
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초록

Background: Considerable evidence suggests that metadherin (MTDH) is a potentially crucial mediator of tumor malignancy and an important therapeutic target for simultaneously enhancing chemotherapy efficacy and reducing metastasis risk. Inhibition of MTDH expression by RNA interference has been shown in several previous research, but silencing MTDH expression by microRNA (miRNA) interference in breast cancer has not been established. In the present study, we investigated the role of MTDH-miRNA in down-regulation of proliferation, motility and migration of breast carcinoma cells. Methods: Expression vectors of recombinant plasmids expressing artificial MTDH miRNA were constructed and transfected to knockdown MTDH expression in MDA-MB-231 breast cancer cells. Expression of MTDH mRNA and protein was detected by RT-PCR and Western blot, respectively. MTT assays were conducted to determine proliferation, and wound healing assays and transwell migration experiments for cell motility and migration. Results: Transfection of recombinant a plasmid of pcDNA-MTDH-miR-4 significantly suppressed the MTDH mRNA and protein levels more than 69% in MDA-MB-231 breast cancer cells. This knockdown significantly inhibited proliferation, motility and migration as compared with controls. Conclusions: MTDH-miRNA may play an important role in down-regulating proliferation, motility and migration in breast cancer cells, and should be considered as a potential small molecule inhibitor therapeutic targeting strategy for the future.

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피인용 문헌

  1. Molecular Modification of Metadherin/MTDH Impacts the Sensitivity of Breast Cancer to Doxorubicin vol.10, pp.5, 2015, https://doi.org/10.1371/journal.pone.0127599
  2. AMPK inhibits MTDH expression via GSK3β and SIRT1 activation: potential role in triple negative breast cancer cell proliferation vol.282, pp.20, 2015, https://doi.org/10.1111/febs.13391
  3. MAP3K1-targeting therapeutic artificial miRNA suppresses the growth and invasion of breast cancer in vivo and in vitro vol.5, pp.1, 2016, https://doi.org/10.1186/s40064-015-1597-z
  4. miR-597 inhibits breast cancer cell proliferation, migration and invasion through FOSL2 vol.37, pp.5, 2017, https://doi.org/10.3892/or.2017.5558
  5. Progress of cancer research on astrocyte elevated gene-1/Metadherin (Review) vol.8, pp.2, 2014, https://doi.org/10.3892/ol.2014.2231