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Biological Activity of Extracts from Acanthopanax sessiliflorum Fruit

오가피(Acanthopanax sessiliflorum) 열매 추출물의 생리활성

  • Jo, Bun-Sung (School of Food Science, Kyungpook National University) ;
  • Cho, Young-Je (School of Food Science & Biotechnology/Food & Bio-Industry Research Institute, Kyungpook National University)
  • 조분성 (경북대학교 식품과학부) ;
  • 조영제 (경북대학교 식품공학부/경북대학교 식품생물산업연구소)
  • Received : 2011.11.25
  • Accepted : 2012.06.22
  • Published : 2012.08.30

Abstract

This study was carried out to determine the biological activity of Acanthopanax sessiliflorum fruit extracts. The phenolic compound contents of the extracts were 21.4 and 15.8 mg/g in hot water and 60% ethanol extracts. The total anti-oxidant activities of the water and the 60% ethanol extracts at a 200 ${\mu}g/mL$ phenolic concent ration were at $92.4{\pm}0.8$ and $89.2{\pm}1.1%$ in terms of the DPPH radical scavenging activity, $98.3{\pm}1.1$ and $96.5{\pm}3.5%$ in terms of the ABTS radical decolorization, $2.0{\pm}0.6$ and $1.2{\pm}2.8$ PF in terms of the anti-oxidant protection factor, and $66.3{\pm}0.8$ and $61.4{\pm}2.3%$ in terms of the TBARs inhibitory activity. The activities that inhibited the angiotensin-converting enzyme and xanthin oxidase were at $85.1{\pm}3.2$ and 0% in the water extracts and $59.3{\pm}1.5$ and $9.5{\pm}0.8%$ in the 60% ethanol extracts at the 200 ${\mu}g/mL$ phenolic concentration. The tyrosinase and elastase inhibitory activities were at $56.6{\pm}1.8$ and $53.1{\pm}1.1%$ in the water extracts and $33.7{\pm}2.2$ and $22.4{\pm}3.1%$ in the 60% ethanol extracts. The astringent effect of the water and the 60% ethanol extracts were at $50.5{\pm}0.9$ and $11.5{\pm}4.1%$.

오가피열매 추출물의 다양한 생리활성을 측정하였다. 오가피열매 추출물의 페놀성물질 함량은 열수 추출물과 60% ethanol 추출물에서 각각 21.4, 15.8 mg/g으로 측정되었으며, 항산화효과 측정에서는 200 ${\mu}g/mL$ 농도의 페놀성 화합물이 첨가되었을 때 DPPH에 대한 전자공여능은 열수 추출물에서는 $92.4{\pm}0.1%$, 60% ethanol 추출물에서는 $89.2{\pm}1.1%$로 측정되었다. $ABTS^+$ radical decolorlization에서는 열수 추출물은 $98.3{\pm}1.1%$, 60% ethanol 추출물은 $96.5{\pm}3.5%$로 측정되었고, antioxidant protection factor측정 결과, 열수 추출물은 $2.0{\pm}0.6$ PF, 60% ethanol 추출물은 $1.2{\pm}2.8$ PF로 측정되었다. TBARs 측정에서는 열수 추출물이 $66.3{\pm}0.8%$, 60% ethanol 추출물이 $61.4{\pm}2.3%$의 저해능을 나타내었다. Angiotensin converting enzyme (ACE)과 xanthine oxidase (XOase) 저해능은 200 ${\mu}g/mL$ 농도의 페놀성 화합물이 첨가 되었을 때 열수 추출물에서 ACE가 $59.3{\pm}1.5%$의 저해능을 나타내었고, 에탄올 추출물에서 ACE가 $85.1{\pm}3.2%$, XOase 가 $9.52{\pm}0.8%$의 저해능을 가지는 것으로 확인되었다. 오가피열매 추출물의 tyrosinase 억제효과는 열수 추출물에서 $56.6{\pm}1.8%$, 60% ethanol 추출물에서 $33.7{\pm}2.2%$의 저해능을 나타내었고, elastase의 경우 열수 추출물과 60% ethanol 추출물에서 각각 $53.1{\pm}1.1%$$22.4{\pm}3.1%$의 저해능을 나타내었다. Astringent 효과는 열수 추출물에서 $50.5{\pm}0.9%$, 60% ethanol 추출물에서 $11.5{\pm}4.1%$의 효과를 가지는 것으로 확인되었다.

Keywords

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