Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Protective Effect of Palmul-tang on Glutamate Induced Cytotoxicity in C6 Glial cells

Glutamate로 유도된 C6 glial 세포의 독성에 대한 팔물탕(八物湯)의 보호 효과

  • Shin, Yong-Jeen (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Shin, Sun-Ho (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University)
  • 신용진 (원광대학교 한의과대학 심계내과학교실) ;
  • 신선호 (원광대학교 한의과대학 심계내과학교실)
  • Received : 2012.03.23
  • Accepted : 2012.08.06
  • Published : 2012.08.25
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Abstract

This study was designed to elucidate the mechanism of the cytoprotective effect of the Palmul-tang (PMT) on glutamate induced cytotoxicity in rat C6 glial cells. We determined the increase of cell viability by PMT on glutamate-induced death of C6 glial cell. On some experiments, glutamate induced cell death to be an apoptotic phenomena characterized by G1 arrest in cell cycle, chromatin condensation, DNA fragmentation in C6 glial cells. However, pre-treatment of PMT inhibited characteristic apoptotic phenomena. One of the main mediator of glutamate-induced cytotoxicity was known to generation of reactive oxigen species. In this study, PMT attenuated generation of reactive oxigen species by glutamate through down-regulation of NOX1 expression in C6 glial cells. Furthermore, PMT regulated Bcl2 families and caspase proteins, which contribute the cell survival or death. This study suggests that PMT may be candidate for both of therapeutic and protective prescription.

Keywords

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