The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Growth Promotion of Pepper Plants by Pantoea ananatis B1-9 and its Efficient Endophytic Colonization Capacity in Plant Tissues

  • Kim, Su-Nam (Department of Applied Biology & Environmental Sciences, Gyeongsang National University) ;
  • Cho, Won-Kyong (Department of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Won-Il (Department of Applied Biology & Environmental Sciences, Gyeongsang National University) ;
  • Jee, Hyeong-Jin (Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration (RDA)) ;
  • Park, Chang-Seuk (Department of Applied Biology & Environmental Sciences, Gyeongsang National University)
  • Received : 2012.02.20
  • Accepted : 2012.06.20
  • Published : 2012.09.01
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Abstract

The bacteria B1-9 that was isolated from the rhizosphere of the green onion could promote growth of pepper, cucumber, tomato, and melon plants. In particular, pepper yield after B1-9 treatment on the seedling was increased about 3 times higher than that of control plants in a field experiment. Partial 16S rDNA sequences revealed that B1-9 belongs to the genus Pantoea ananatis. Pathogenecity tests showed non-pathogenic on kimchi cabbage, carrot, and onion. The functional characterization study demonstrated B1-9's ability to function in phosphate solubilization, sulfur oxidation, nitrogen fixation, and indole-3-acetic acid production. To trace colonization patterns of B1-9 in pepper plant tissues, we used $DRAQ5^{TM}$ fluorescent dye, which stains the DNAs of bacteria and plant cells. A large number of B1-9 cells were found on the surfaces of roots and stems as well as in guard cells. Furthermore, several colonized B1-9 cells resided in inner cortical plant cells. Treatment of rhizosphere regions with strain B1-9 can result in efficient colonization of plants and promote plant growth from the seedling to mature plant stage. In summary, strain B1-9 can be successfully applied in the pepper plantation because of its high colonization capacity in plant tissues, as well as properties that promote efficient plant growth.

Keywords

References

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