Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
권호 표지

Acupuncture Treatment at HT8 Supresses Seizure and Inflammation in Hippocampi on an Epilepsy Mice Model

간질(癎疾) 동물(動物) 모델을 이용한 소부혈(少府穴)의 간질발작(癎疾發作) 및 해마(海馬)의 염증(炎症) 억제 효과(效果) 검증(檢證)

  • Doo, Ah-Reum (Dept. of Meridian & Acupoint, College of Korean Medicine) ;
  • Kim, Seung-Nam (Dept. of Meridian & Acupoint, College of Korean Medicine) ;
  • Yin, Chang-Shik (Acupuncture & Meridian Science Research Center, Kyung Hee University) ;
  • Kim, Yeon-Jung (College of Nursing Science, Kyung Hee University) ;
  • Lee, Hye-Jung (Dept. of Meridian & Acupoint, College of Korean Medicine) ;
  • Kim, Seung-Tae (Division of Meridian and Structural Medicine, School of Korean Medicine, Pusan National University) ;
  • Park, Hi-Joon (Dept. of Meridian & Acupoint, College of Korean Medicine)
  • 두아름 (경희대학교 한의과대학 경혈학교실) ;
  • 김승남 (경희대학교 한의과대학 경혈학교실) ;
  • 인창식 (경희대학교 침구경락과학연구센터) ;
  • 김연정 (경희대학교 간호과학대학) ;
  • 이혜정 (경희대학교 한의과대학 경혈학교실) ;
  • 김승태 (부산대학교 한의학전문대학원 경락구조의학부) ;
  • 박히준 (경희대학교 한의과대학 경혈학교실)
  • Received : 2012.08.13
  • Accepted : 2012.09.03
  • Published : 2012.09.27
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Abstract

Objectives : To confirm whether acupuncture can suppress the degree of seizure and the activation of astrocytes in hippocampi using kainic acid(KA)-induced epilepsy mouse model. Methods : 8 weeks C57BL/6 mice(20~25 g) were given acupuncture once a day at acupoint HT8(Shaofu) bilaterally during 2 days before KA injection. After an intraperitoneal injection of 30 mg/kg KA, acupuncture treatment was subsequently administered once more(total 3 times), and the degree of seizure was observed for 120 min. The neuronal cell death, pERK expression, and astrocyte activation confirmed 1 hour and 24 hours after KA injection. Results : Acupuncture treatment at HT8 suppressed KA-induced epileptic seizure. One hour after KA injection, the pERK expression was increased, which was reduced by the acupuncture treatment. Twenty four hours after injection, the treatment decreased the KA-induced neuronal cell death, the interleukin-$1{\beta}$ expression and the astrocyte activation in the CA3 region of the mouse hippocampus. Conclusions : Acupuncture treatment at HT8 decreases the KA-induced epileptic seizure, the neural cell inflammation and death.

Keywords

References

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