Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Inhibitory Effects of Marine Algae Extract on Adipocyte Differentiation and Pancreatic Lipase Activity

  • Kim, Eun-Sil (Wildlife Genetic Resources Center, National Institute of Biological Resources) ;
  • Lee, Kyoung-Jin (Wildlife Genetic Resources Center, National Institute of Biological Resources) ;
  • Oh, Kyoung-Hee (Wildlife Genetic Resources Center, National Institute of Biological Resources) ;
  • Ahn, Jong-Hoon (College of Pharmacy, Chungbuk National University) ;
  • Kim, Seon-Beom (College of Pharmacy, Chungbuk National University) ;
  • Liu, Qing (College of Pharmacy, Chungbuk National University) ;
  • Hwang, Bang-Yeon (College of Pharmacy, Chungbuk National University) ;
  • Lee, Mi-Kyeong (College of Pharmacy, Chungbuk National University)
  • Received : 2012.05.23
  • Accepted : 2012.06.30
  • Published : 2012.09.30
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Abstract

Obesity, which is characterized by excessive fat accumulation in adipose tissues, occurs by fat absorption by lipase and sequential fat accumulation in adipocyte through adipocyte differentiation. Thus, inhibition of pancreatic lipase activity and adipocyte differentiation would be crucial for the prevention and progression of obesity. In the present study, we attempted to evaluate anti-adipogenic activity of several algae extracts employing preadipocytes cell line, 3T3-L1 as an in vitro assay system. The effects on pancreatic lipase activity in vitro were also evaluated. Total methanolic extracts of Cladophora wrightiana and Costaria costata showed significant inhibitory activity on adipocyte differentiation as assessed by measuring fat accumulation using Oil Red O staining. Related to pancreatic lipase, C. wrightiana and Padina arborescens showed significant inhibition. Further fractionation of C. wrightiana, which showed the most potent activity, suggested that $CHCl_3$ and n-BuOH fraction are responsible for adipocyte differentiation inhibition, whereas n-BuOH and $H_2O$ fraction for pancreatic lipase inhibition. Our study also demonstrated that n-BuOH fraction was effective both in early and middle stage of differentiation whereas $CHCl_3$ fraction was effective only in early stage of differentiation. Taken together, algae might be new candidates in the development of obesity treatment.

Keywords

References

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