Preventive Nutrition and Food Science

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Methanol Extract of Cassia mimosoides var. nomame Attenuates Myocardial Injury by Inhibition of Apoptosis in a Rat Model of Ischemia-Reperfusion

  • Lim, Sun-Ha (Department of Biochemistry, School of Medicine, Catholic University of Daegu) ;
  • Lee, Jong-Won (Department of Biochemistry, School of Medicine, Catholic University of Daegu)
  • Received : 2012.05.14
  • Accepted : 2012.09.01
  • Published : 2012.09.30
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Abstract

Interruption of blood flow through coronary arteries and its subsequent restoration triggers the generation of a burst of reactive oxygen species (ROS), leading to myocardial cell death. In this study, we determined whether a methanol extract of Cassia mimosoides var. nomame Makino could prevent myocardial ischemia-reperfusion injury. When radical scavenging activity of the extract was measured in vitro using its ${\alpha}$,${\alpha}$-diphenyl-${\beta}$-picrylhydrazyl (DPPH) radical quenching ability, the extract showed an activity slightly lower than that of ascorbic acid. Three days after oral administration of the extract (400 mg/kg/day) to rats, myocardial ischemia/reperfusion injury was generated by 30 min of ligation of the left anterior descending coronary artery (LAD), followed by 3 hr reperfusion. Compared with the vehicle-treated group, administration of the extract significantly reduced infarct size (IS) (ratio of infarct area to area at risk) in the extract-treated group by 28.3%. Reduction in the cellular injury was mediated by attenuation of Bax/Bcl-2 ratio by 33.3%, inhibition of caspase-3 activation from procaspase-3 by 40%, and subsequent reduction in the number of apoptotic cells by 66.3%. These results suggest that the extract attenuates myocardial injury in a rat model of ischemia-reperfusion by scavenging ROS, including free radicals, and consequently blocking apoptotic cascades. Therefore, intake of Cassia mimosoides var. nomame Makino might be beneficial for preventing ischemic myocardial injury.

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References

  1. Abbate A, Bussani R, Amin MS, Vetrovec GW, Baldi A. 2006. Acute myocardial infarction and heart failure: role of apoptosis. Int J Biochem Cell Biol 38: 1834-1840. https://doi.org/10.1016/j.biocel.2006.04.010
  2. Cohen M, Boiangiu C, Abidi M. 2010. Therapy for ST-segment elevation myocardial infarction patients who present late or are ineligible for reperfusion therapy. J Am Coll Cardiol 55: 1895-1906. https://doi.org/10.1016/j.jacc.2009.11.087
  3. Nadtochiy SM, Redman EK. 2011. Mediterranean diet and cardioprotection: the role of nitrite, polyunsaturated fatty acids, and polyphenols. Nutrition 27: 733-744. https://doi.org/10.1016/j.nut.2010.12.006
  4. Raedschelders K, Ansley DM, Chen DDY. 2012. The cellular and molecular origin of reactive oxygen species generation during myocardial ischemia and reperfusion. Pharmacol Ther 133: 230-235. https://doi.org/10.1016/j.pharmthera.2011.11.004
  5. Andreadou I, Iliodromitis EK, Farmakis D, Kremastinos DT. 2009. To prevent, protect and save the ischemic heart: antioxidants revisited. Expert Opin Ther Targets 13: 945- 956. https://doi.org/10.1517/14728220903039698
  6. Kumar D, Lou H, Singal PK. 2002. Oxidative stress and apoptosis in heart dysfunction. Herz 27: 662-668. https://doi.org/10.1007/s00059-002-2430-3
  7. Portt L, Norman G, Clapp C, Greenwood M, Greenwood MT. 2011. Anti-apoptosis and cell survival: a review. Biochim Biophysic Acta 1813: 238-259. https://doi.org/10.1016/j.bbamcr.2010.10.010
  8. Park JH, Kwon SJ. 2009. Isolation of daucosterol and napthlene glucoside from seeds of Cassia mimosoides var. nomame Makino. Korean J Plant Res 22: 26-30.
  9. Yamamoto M, Shimura S, Itoh T, Ohsaka T, Egawa M, Inoue S. 2000. Anti-obesity effects of lipase inhibitor CTII, an extract from edible herbs, Nomame Herba, on rats fed a high-fat diet. Int J Obes 24: 758-764. https://doi.org/10.1038/sj.ijo.0801222
  10. Kim KH, Lee JW. 2010. Methanol extract of Cassia mimosoides var. nomame and its ethyl acetate fraction attenuate brain damage by inhibition of apoptosis in a rat model of ischemia-reperfusion. J Food Sci Nutr 15: 255-261. https://doi.org/10.3746/jfn.2010.15.4.255
  11. Jung HJ, Kim MJ, Lim SH, Park JH, Lee HK, Ahn KS, Lee JW. 2009. The effect of extract of Paeonia lactiflora on the improvement of ischemic acute renal failure. Korean J Nephrol 28: 180-189.
  12. Kuriakose GC, Kurup MG. 2010. Effects of Aulosira fertilisima against cisplatin-induced nephrotoxicity and oxidative stress in rats. Ren Fail 32: 224-233. https://doi.org/10.3109/08860220903541143
  13. Lim SH, Song KS, Lee JW. 2010. Butyrate and propionate, short chain fatty acids, attenuate myocardial damages by inhibition of apoptosis in a rat model of ischemiareperfusion. J Korean Soc Appl Biol Chem 53: 570-577. https://doi.org/10.3839/jksabc.2010.088
  14. Tao L, Gao E, Bryan NS, Qu Y, Liu HR, Hu A, Christopher TA, Lopez BL, Yodoi J, Koch WJ, Feelisch M, Ma XL. 2004. Cardioprotective effects of thioredoxin in myocardial ischemia and reperfusion: role of S-nitrosation. Proc Natl Acad Sci 101: 11471-11476 https://doi.org/10.1073/pnas.0402941101
  15. Harada M, Qin Y, Takano H, Minamino T, Zou Y, Toko H, Ohtsuka M, Matsuura K, Sano M, Nishi J, Iwanaga K, Akazawa H, Kunieda T, Zhu W, Hasegawa H, Kunisada K, Nagai T, Nakaya H, Yamauchi-Takihara K, Komuro I. 2005. G-CSF prevents cardiac remodeling after myocardial infarction by activating the Jak-Stat pathway in cardiomyocytes. Nat Med 11: 305-311. https://doi.org/10.1038/nm1199
  16. Shibata R, Sato K, Pimentel DR, Takemura Y, Kihara S, Ohashi K, Funahashi T, Ouchi N, Walsh K. 2005. Adiponectin protects against myocardial ischemia-reperfusion injury through AMPK- and COX-2-dependent mechanisms. Nat Med 11: 1096-1103. https://doi.org/10.1038/nm1295
  17. Krijnen PAJ, Nijmeijer R, Meijer CJLM, Visser CA, Hack CE, Niessen HWM. 2002. Apoptosis in myocardial ischemia and infarction. J Clin Pathol 55: 801-811. https://doi.org/10.1136/jcp.55.11.801
  18. Crow MT, Mani K, Nam YJ, Kitsis RN. 2004. The mitochondrial death pathway and cardiac myocyte apoptosis. Circ Res 95: 957-970. https://doi.org/10.1161/01.RES.0000148632.35500.d9
  19. Zidar N, Dolenc-Strazar Z, Jeruc J, Stajer D. 2006. Immunohistochemical expression of activated caspase-3 in human myocardial infarction. Virchows Arch 448: 75-79. https://doi.org/10.1007/s00428-005-0073-5
  20. Rabkin SW. 2009. Apoptosis in human acute myocardial infarction: the rationale for clinical trials of apoptosis inhibition in acute myocardial infarction. Scholarly Research Exchange doi: 10.3814/2009/979318.
  21. Hengartner MO. 2000. The biochemistry of apoptosis. Nature 407: 770-776. https://doi.org/10.1038/35037710
  22. Taylor RC, Cullen SP, Martin SJ. 2008. Apoptosis: controlled demolition at the cellular level. Nat Rev Mol Cell Biol 9: 231-241. https://doi.org/10.1038/nrm2312
  23. Spierings D, McStay G, Saleh M, Bender C, Chipuk J, Mauer U, Green DR. 2005. Connected to death: the (unexpurgated) mitochondrial pathway of apoptosis. Science 310: 66-67. https://doi.org/10.1126/science.1117105
  24. Lakhani SA, Masud A, Kuida K, Porter Jr GA, Booth CJ, Mehal WZ, Inayat I, Flavell RA. 2006. Caspases 3 and 7: key mediators of mitochondrial events of apoptosis. Science 311: 847-851. https://doi.org/10.1126/science.1115035
  25. Adams JM, Cory S. 2001. Life-or-death decisions by the Bcl-2 protein family. Trends Biochem Sci 26: 61-66. https://doi.org/10.1016/S0968-0004(00)01740-0
  26. Empel VPM, Bertrand ATA, Hofstra L, Crijins HJ, Doevendans PA, Windt LJ. 2005. Myocyte apoptosis in heart failure. Cardiovasc Res 67: 21-29. https://doi.org/10.1016/j.cardiores.2005.04.012
  27. Lee Y, Gustafsson AB. 2009. Role of apoptosis in cardiovascular disease. Apoptosis 14: 536-548. https://doi.org/10.1007/s10495-008-0302-x
  28. Oltvai ZN, Milliman CL, Korsmeyer SJ. 1993. Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell 74: 609-619. https://doi.org/10.1016/0092-8674(93)90509-O
  29. Ahmad R, Javed S, Bhandari U. 2010. Antiapoptotic potential of herbal drugs in cardiovascular disorders: an overview. Pharm Biol 48: 358-374. https://doi.org/10.3109/13880200903133852
  30. Bangou MJ, Kiendrebeogo M, Compaore M, Coulibaly AY, Meda NTR, Abarca NA, Zeba B, Millogo-Rasolodimby J, Nacoulma OG. 2011. Enzyme inhibition effect and polyphenolic content of medicinal plant extracts from Burkino Faso. J Biol Sci 11: 31-38. https://doi.org/10.3923/jbs.2011.31.38
  31. Kuang W, Zhang C, Huang MR, Liu ZW. 2011. Antioxidant activities of the 95% ethanol extracts of nine Chinese herbs commonly used in Hakka area. Adv Mater Res 396- 398: 246-249. https://doi.org/10.4028/www.scientific.net/AMR.396-398.246
  32. El-Hashash MM, Abdel-Gawad MM, El-Sayed MM, Sabry WA, Abdel-Hameed ES, Abdel-Lateef EE. 2010. Antioxidant properties of methanolic extracts of the leaves of seven Egyptian Cassia species. Acta Pharm 60: 361-367. https://doi.org/10.2478/v10007-010-0030-y

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