Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antioxidant Activity of Extracts and Fractions from Aster scaber

참취 추출물과 용매분획물의 항산화 활성

  • Jeon, Sang-Min (Functional Food and Nutrition Division, Dept. of Agrofood Resources, Rural Development Administration) ;
  • Lee, Jin-Young (Functional Food and Nutrition Division, Dept. of Agrofood Resources, Rural Development Administration) ;
  • Kim, Heon-Woong (Functional Food and Nutrition Division, Dept. of Agrofood Resources, Rural Development Administration) ;
  • Lee, Young-Min (Functional Food and Nutrition Division, Dept. of Agrofood Resources, Rural Development Administration) ;
  • Jang, Hwan-Hee (Functional Food and Nutrition Division, Dept. of Agrofood Resources, Rural Development Administration) ;
  • Hwang, Kyung-A (Functional Food and Nutrition Division, Dept. of Agrofood Resources, Rural Development Administration) ;
  • Kim, Haeng-Ran (Functional Food and Nutrition Division, Dept. of Agrofood Resources, Rural Development Administration) ;
  • Park, Dong-Sik (Functional Food and Nutrition Division, Dept. of Agrofood Resources, Rural Development Administration)
  • 전상민 (농촌진흥청 국립농업과학원 기능성식품과) ;
  • 이진영 (농촌진흥청 국립농업과학원 기능성식품과) ;
  • 김헌웅 (농촌진흥청 국립농업과학원 기능성식품과) ;
  • 이영민 (농촌진흥청 국립농업과학원 기능성식품과) ;
  • 장환희 (농촌진흥청 국립농업과학원 기능성식품과) ;
  • 황경아 (농촌진흥청 국립농업과학원 기능성식품과) ;
  • 김행란 (농촌진흥청 국립농업과학원 기능성식품과) ;
  • 박동식 (농촌진흥청 국립농업과학원 기능성식품과)
  • Received : 2012.04.25
  • Accepted : 2012.06.04
  • Published : 2012.09.30
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Abstract

As an effort to develop functional food ingredients and to discover the biological activity, the total phenolic content, total flavonoid content, DPPH and ABTS radical scavenging activity, SOD-like activity, ferric reducing antioxidant power (FRAP), and $Fe^{2+}$ chelating of Aster scaber were measured using a 70% ethanol extract and various solvent fractions. As a result, the total phenolic concent was highest in an ethyl acetate fraction of 141.9 mg GAE eq/g and the total flavonoid content was 105.6 mg QUE eq/g. The DPPH radical scavenging activity was highest in an ethyl acetate fraction of 97.1% at a concentration of 1,000 ${\mu}g/mL$ (p<0.05). The ABTS radical scavenging activity showed a 86.9% ethyl acetate fraction and a 57.9% butanol fraction at a concentration of 125 ${\mu}g/mL$, and higher than that of positive control (${\alpha}$-tocopherol and BHT) (p<0.05). The SOD-like activity showed 42.8% in an ethyl acetate at a concentration of 1,000 ${\mu}g/mL$. The ethyl acetate fraction showed the highest value of FRAP at 1051.9 ${\mu}M$ and a concentration of 1,000 ${\mu}g/mL$ (p<0.05). The $Fe^{2+}$chelating was highest in the 70.1% chloroform fraction at a concentration of 500 ${\mu}g/mL$ (p<0.05). There is the highest correlation between DPPH radical scavenging activity and FRAP (r=0.981) as compared to other antioxidant assays (p<0.01). With these results, we confirmed that the ethyl acetate fraction of Aster scaber has great antioxidant potential. So it can be expected to be developed into a specific functional food ingredient.

본 연구는 참취 에탄올 추출물과 용매분획물의 항산화 활성과 총 폴리페놀 함량 및 총 플라보노이드 함량을 측정하고, 생리활성과 항산화 성분 간의 상관관계를 비교 분석하였다. 총 폴리페놀 함량은 70% 에탄올 추출물 99.6 mg GAE eq/g으로 나타났으며, ethyl acetate 분획물 141.9 mg GAE eq/g으로 다른 분획물보다 높은 함량을 나타내었다(p<0.05). 또한 총 플라보노이드 함량도 ethyl acetate 분획물에서 105.6 mg QUE eq/g으로 높은 함량을 나타내었다(p<0.05). DPPH 라디칼 소거능과 ABTS 라디칼 소거능의 경우 ethyl acetate 분획물 1,000 ${\mu}g/mL$ 농도에서 97.1% 및 98.7%를 보여 대조군인 ascorbic acid, ${\alpha}$-tocopherol 및 BHT와 유사한 소거능을 나타내었다(p<0.05). SOD 유사활성은 ethyl acetate 분획물 1,000 ${\mu}g/mL$ 농도에서 42.8%의 활성을 나타내어 DPPH, ABTS 라디칼 소거능보다 비교적 낮은 활성을 나타내었다(p<0.05). FRAP에 의한 환원력 측정 결과에서도 ethyl acetate 분획물에서 높은 활성을 나타내었다(p<0.05). 그러나 $Fe^{2+}$ chelating 활성 측정은 chloroform 분획물 1,000 ${\mu}g/mL$ 농도에서 81.8%로 높게 나타났으며, 오히려 ethyl acetate 분획물에서는 6.2%로 가장 낮게 나타났다(p<0.05). 이상의 결과 참취 70% 에탄올 추출물과 용매 분획물 중 ethyl acetate 분획물이 다량의 폴리페놀과 플라보노이드를 함유하고 있으며, 항산화 효과가 높게 나타났다. 따라서 참취는 천연 항산화 소재 및 기능성식품으로의 활용 가능한 약용 식물자원인 것으로 판단된다.

Keywords

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