Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Study on Antioxidant Effects of Fractional Extracts from Ligularia stenocephala Leaves

곤달비(Ligularia stenocephala) 잎 분획물의 항산화활성 평가

  • Kim, Kyoung-Hee (Dept. Of Food and Nutrition, Chungnam National University) ;
  • Kim, Na-Young (Dept. of Food and Nutrition, Jungbu University) ;
  • Kim, Sung-Hwan (Dept. of Food and Nutrition, Jungbu University) ;
  • Han, In-Ae (Dept. Of Food and Nutrition, Chungnam National University) ;
  • Yook, Hong-Sun (Dept. Of Food and Nutrition, Chungnam National University)
  • 김경희 (충남대학교 식품영양학과) ;
  • 김나영 (중부대학교 식품영양학과) ;
  • 김성환 (중부대학교 식품영양학과) ;
  • 한인애 (충남대학교 식품영양학과) ;
  • 육홍선 (충남대학교 식품영양학과)
  • Received : 2012.05.08
  • Accepted : 2012.06.18
  • Published : 2012.09.30
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Abstract

Ligularia stenocephala leaves were subjected to extraction by using several solvents with different polarities, which were then investigated for their antioxidant and antimicrobial activities. Extraction yield of ethanol extract of Ligularia stenocephala was 21.36%. The water fraction showed the highest extraction yield of 60.35%, followed by the n-butanol, n-hexane, and ethyl acetate fractions. Total polyphenolic content was the highest (254.00 mg/g GAE) in the ethyl acetate fraction of Ligularia stenocephala extract. The ethyl acetate fraction had $IC_{50}$ values of 0.28 mg/mL for DPPH radical scavenging, and 96.$67{\pm}0.09%$ for ABTS radical scavenging activity. Further, FRAP value was significantly higher in the ethyl acetate fraction. The ethyl acetate fraction showed antimicrobial activities against B. cereus, and the diameter of the zone of inhibition was 10.2 mm at 5 mg/disc. These results suggest that the ethyl acetate fraction of Ligularia stenocephala possesses the antimicrobial activities against B. cereus, and its high antioxidant activity could be applicable to food additives and as a natural cosmetic ingredient.

본 연구는 곤달비(Ligularia stenocephala)의 생리 효능을 체계적으로 밝히기 위한 기초 자료를 얻고자 80% 에탄올 추출물과 순차적 분획물에 대하여 항산화 및 항균 효과를 검증하였다. 곤달비 에탄올 추출물의 추출수율은 21.36%이었으며, 이에 대하여 용매별로 분획한 수율은 water 분획물이 60.35%로 가장 높았으며, n-butanol, n-hexane, ethyl acetate 순이었다. 곤달비 추출물 및 분획물의 총 폴리페놀 함량은 ethyl acetate 분획물에서 254.00 mg/g으로 가장 높게 나타났다. DPPH radical 소거활성을 측정한 결과, 곤달비 ethyl acetate 분획물에서 $IC_{50}$ 값이 0.28 mg/mL로 높은 활성을 나타내었다. 0.5 mg/mL 농도에서의 ABTS 라디칼 소거활성은 ethyl acetate 분획물에서 96.67%의 활성을 보였다. FRAP 활성 역시 1 mg/mL 농도에서 ethyl acetate 분획물이 2.70 mM의 가장 높은 활성을 나타내었다. 항균활성은 B. cereus 균주에 대해 ethyl acetate 분획물 5 mg/disc에서 10.2 mm의 저해환을 나타내었다. 이상의 결과에서 곤달비의 추출물 및 분획물 중 ethyl acetate 분획물의 경우 높은 항산화 활성과 약간의 항균활성을 나타내었으며 천연물소재로서 식품첨가물 및 화장품 원료로의 가능성이 있는 것으로 사료된다.

Keywords

References

  1. Choi J, Kim WB, Nam JH, Park HJ. 2007. Antidiabetic effect of the methanolic extract of Ligularia stenocephala leaves on the streptozotocin induced rat. Korean J Plant Res 20: 362-366.
  2. Kim TJ. 1996. Korean resources plants. The publishing center of Seoul National University, Seoul, Korea. p 242-243.
  3. Roh EJ, Kim YS, Kim BG. 2009. Effect of antioxidation and inhibition of melanogenesis from Ligularia stenocephala extract. J Korean Oil Chemists' Soc 26: 87-92.
  4. Lee YH. 2000. Effect of Ligularia stenocephala extract on lipid metabolism and liver function of rat administered with ethanol. MS Thesis. Dong-A University, Busan, Korea.
  5. Roh EJ, Kim YS, Kim BG. 2009. Anti-wrinkle effect of Ligularia stenocephala. J Korean Oil Chemists' Soc 26: 186-190.
  6. Jung IC. 2006. Rheological properties of white bread supplemented with Ligularia stenocephala leaf powder and its sensory characteristics according to survey panel members. Korean J Food & Nutr 19: 207-218.
  7. Folin O, Denis W. 1912. On phospotungastic-phospho-molybdic compounds as color reagents. J Biol Chem 12: 239-249.
  8. Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181: 1198-1200.
  9. Pellegrin N, Roberta R, Min Y, Catherine RE. 1998. Screening of dietary carotenoids and carotenoid-rich fruit extract for antioxidant activities applying 2,2'-azinobis(3-ethyl-enbenzothiazoline-6-sulfonic acid) radical cation decolorization assay. Method Enzymol 299: 379-389.
  10. Benzie IFF, Strain JJ. 1996. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": The FRAP assay. Anal Biochem 230: 70-79.
  11. Sa JH, Jin YS, Shim IC, Wang MH. 2004. Photoprotective effect and antioxidative activity from different organs of Morcus bombycis Koidzumi. Kor J Pharmacogn 35: 207-214.
  12. Lee YM, Lee JJ, Lee MY. 2008. Antioxidative effect of Pimpinella brachycarpa ethanol extract. J Life Sci 18: 467-473. https://doi.org/10.5352/JLS.2008.18.4.467
  13. Lee SH, Jin YS, Heo SH, Shim TH, Sa JH, Choi DS, Wang MH. 2006. Composition analysis and antioxidative activity from different argans of Cirsium setidens Nakai. Korean J Food Sci Technol 38: 571-576.
  14. Yu MH, Im HG, Lee HJ, Ji YJ, Lee IS. 2006. Components and their antioxidative activites of methanol extracts form asrcocarp and seed of Zizyphus jujuba var. inermis rehder. Korean J Food Sci Technol 38: 128-134.
  15. Lee SO, Lee HJ, Yu MH, Im HG, Lee IS. 2005. Total polyphenol contents and antioxidant activities of methanol extracts from vegetables produced in Ullung island. Korean J Food Sci Technol 37: 233-240.
  16. Choi YM, Kim MH, Shin JJ, Park JM, Lee JS. 2003. The antioxidant activities of the some commercial teas. J Korean Soc Food Sci Nutr 32: 723-727. https://doi.org/10.3746/jkfn.2003.32.5.723
  17. Seo YH, Kim IJ, Yie AS, Min HK. 1999. Electron donating ability and contents of phenolic compounds, tocopherols and carotenoids in waxy corn (Zea mays L.). Korean J Food Sci Technol 31: 581-585.
  18. Wang MF, Shao Y, Li JG, Zhu NQ, Rngarajan M, Lavoie EJ, Ho CT. 1998. Antioxidative phenolic compounds from sage (Salivia officinalis). J Agric Food Chem 46: 4869-4873. https://doi.org/10.1021/jf980614b
  19. Choi SS, Yim DS, Lee SY. 2009. Radical scavenging activities and protective effects against oxidative damage to DNA of extracts from medicinal plants with known osteoprotective effects. Kor J Pharmacogn 40: 143-149.
  20. Lee SE, Kim YS, Kim JE, Bang JK, Seong NS. 2004. Antioxidant activity of Ulmus davidiana var. japonica N. and Hemipteleae davidii P. J Korean Medicinal Crop Sci 12: 321-327.
  21. Li HB, Wong CC, Cheng KW, Chen F. 2008. Antioxidant properties in vitro and total phenolic contents in methanol extracts from medicinal plants. LWT-Food Sci Technol 41: 385-390. https://doi.org/10.1016/j.lwt.2007.03.011
  22. Kang YH, Park YK, Lee GD. 1996. The nitrite scavenging and electron donating ability of phenolic compounds. Korean J Food Sci Technol 33: 626-632.
  23. Cho YH, Park HS, Choi MK, Chiang MH. 2001. Anti-microbial activities of Korean herb plants. Kor J Hort Sci Supplement 19: 80.
  24. Lim HA, Yoon SI. 2009. Antimicrobial activities of Capsella bursa-pastoris extracts. Korean J Food Preserv 16: 562-566.

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