Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Rubus coreanus Miquel Improves on Impairment of Memory in Senescence-Accelerated Mouse (SAM)

노화촉진마우스를 이용한 복분자의 기억력 감퇴 개선 효과

  • Choi, Mi-Ran (Center for Efficacy Assessment and Development of Functional Foods and Drugs) ;
  • Lee, Min-Young (Center for Efficacy Assessment and Development of Functional Foods and Drugs) ;
  • Kim, Jeong-Eun (Center for Efficacy Assessment and Development of Functional Foods and Drugs) ;
  • Hong, Ji-Eun (Center for Efficacy Assessment and Development of Functional Foods and Drugs) ;
  • Jang, Kuen-Hye (Center for Efficacy Assessment and Development of Functional Foods and Drugs) ;
  • Lee, Jae-Yong (Center for Efficacy Assessment and Development of Functional Foods and Drugs) ;
  • Chun, Jang-Woo (Research and Development Center, Hitejinro Co., Ltd.) ;
  • Kim, Tae-Hwan (Research and Development Center, Hitejinro Co., Ltd.) ;
  • Shin, Hyun-Kyung (Center for Efficacy Assessment and Development of Functional Foods and Drugs) ;
  • Kim, Eun-Ji (Center for Efficacy Assessment and Development of Functional Foods and Drugs)
  • 최미란 (한림대학교 식의약품의 효능평가 및 기능성소재개발센터) ;
  • 이민영 (한림대학교 식의약품의 효능평가 및 기능성소재개발센터) ;
  • 김정은 (한림대학교 식의약품의 효능평가 및 기능성소재개발센터) ;
  • 홍지은 (한림대학교 식의약품의 효능평가 및 기능성소재개발센터) ;
  • 장근혜 (한림대학교 식의약품의 효능평가 및 기능성소재개발센터) ;
  • 이재용 (한림대학교 식의약품의 효능평가 및 기능성소재개발센터) ;
  • 전장우 (하이트진로주식회사 중앙연구소) ;
  • 김태환 (하이트진로주식회사 중앙연구소) ;
  • 신현경 (한림대학교 식의약품의 효능평가 및 기능성소재개발센터) ;
  • 김은지 (한림대학교 식의약품의 효능평가 및 기능성소재개발센터)
  • Received : 2012.05.11
  • Accepted : 2012.07.16
  • Published : 2012.09.30
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Abstract

In the present study, we investigated the effects of Rubus coreanus Miquel (RCM) on memory ability of senescence-accelerated mice (SAM). Prone 8 strains of SAM mice (SAMP8), which is a useful animal for investigating the mechanism of brain aging and senile dementia, were fed a diet containing 100 mg/kg body weight/day of RCM for 8 weeks. Memory ability of mice was examined by using passive avoidance test and Morris water maze test. SAMP8 mice showed remarkable memory impairment compared with senescence-resistant 1 strains of SAM (SAMR1). RCM significantly improved memory ability of SAMP8 mice. In addition, acetylcholineasterase activities decreased in the brain of SAMP8 mice treated with RCM. Taken together, these results suggest that RCM may act as an acetylcholineasterase inhibitor, thereby improving senescence-related memory impairment.

본 연구에서는 복분자 섭취가 노화에 의한 기억력 감퇴에 개선 효과를 나타내는지 조사하기 위해 노화촉진마우스 (SAM)를 사용하여 평가하였다. 28주령 SAMP8 마우스에 복분자 시료를 100 mg/kg body weight/day로 8주간 투여한 후 수동회피시험과 수중미로시험을 실시하여 기억력 개선효과를 평가하였다. 수동회피시험에서 노화 촉진 동물(SAMP8)은 대조동물(SAMR1)에 비해 밝은 방에서 체류시간이 현저히 감소하였다. 복분자를 섭취한 노화 촉진 동물의 밝은 방에서의 체류 시간은 복분자를 섭취하지 않은 동물에 비해 현저히 증가하였다. 수중미로실험에서 대조 동물에 비해 노화 촉진 동물의 평균 탈출잠복기는 길었고, 복분자를 섭취한 경우 평균 탈출잠복기가 유의적으로 감소하였다. 뇌의 대뇌피질과 해마 조직에서 대조군에 비해 노화 촉진 동물의 acetylcholinesterase 활성이 증가하였다. 복분자를 섭취한 노화 촉진 동물의 해마 조직 내 acetylcholinesterase는 복분자를 섭취하지 않은 동물에 비해 유의적으로 감소하였다. 이 결과들은 복분자가 노화촉진마우스에서 노화에 의한 기억력 감퇴에 대한 개선 효과를 나타내며, 이는 콜린성 신경전달에 중요한 역할을 하는 acetylcholinesterase 활성 감소를 통해 이루어짐을 나타낸다. 향후 더 많은 연구가 수행 되어야 하나, 본 연구는 복분자가 노인의 기억력 개선제 개발에 좋은 기능성 소재로 활용할 수 있음을 제시한다.

Keywords

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