Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
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Effect on Insecticide Susceptibility of Lissorhoptrus oryzophilus Fed on Carotenoid-Biofortified Rice Variety

비타민 A 강화벼 급이가 벼물바구미(Lissorhoptrus oryzophilus)의 살충제 감수성에 미치는 영향

  • Oh, Sung-Dug (National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Ki-Jong (National Academy of Agricultural Science, Rural Development Administration) ;
  • Park, Soo-Yun (National Academy of Agricultural Science, Rural Development Administration) ;
  • Ryu, Tae-Hun (National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Jae-Kwang (National Academy of Agricultural Science, Rural Development Administration) ;
  • Sohn, Soo-In (National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Jin-Seo (Division of Plant Biosciences, School of Applied Biosciences, Kyungpook National University) ;
  • Ha, Sun-Hwa (National Academy of Agricultural Science, Rural Development Administration) ;
  • Park, Jong-Sug (National Academy of Agricultural Science, Rural Development Administration) ;
  • Ahn, Byung-Ohg (National Academy of Agricultural Science, Rural Development Administration) ;
  • Cho, Hyun-Suk (National Academy of Agricultural Science, Rural Development Administration) ;
  • Suh, Sang-Jae (Division of Plant Biosciences, School of Applied Biosciences, Kyungpook National University)
  • 오성덕 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 이기종 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 박수윤 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 류태훈 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 김재광 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 손수인 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 김진서 (경북대학교 농업생명과학대학 응용생명과학부) ;
  • 하선화 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 박종석 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 안병옥 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 조현석 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 서상재 (경북대학교 농업생명과학대학 응용생명과학부)
  • Received : 2012.07.07
  • Accepted : 2012.09.21
  • Published : 2012.09.30
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Abstract

BACKGROUND: The carotenoid-biofortified (PAC) rice was generated by inserting phytoene synthase (Psy) and carotene desaturase (Crtl) genes isolated from Capsicum annuum cv. Nockwang and Pantoea ananatis into the genome of a conventional variety of rice (Nakdongbyeo). In our present study, we studied the effects on insecticide susceptibility of Rice Water Weevil (Lissorhoptrus oryzophilus). METHODS AND RESULTS: The L. oryzophilus were fed on carotenoid-biofortified (PAC) rice and its near non-genetically modified (GM) counterparts (Nakdongbyeo) under $25{\pm}1^{\circ}C$, humidity of $60{\pm}5%$, and photoperiod 16L:8D for more than 60 days. Ten adults were soaked in the Clothianidin SC solution for 5 second in different concentrations, and were detected the mortalities after 24, 48 and 72 hours respectively. Every experiment was conducted with three replications. The cumulative mortalities of L. oryzophilus exposed to Clothianidin SC were similar between two types of feed administration. CONCLUSION: The results of this study suggested that carotenoid-biofortified rice might not affect the insecticide susceptibilities of Lissorhoptrus oryzophilus.

비타민A 강화벼와 낙동벼의 벼물바구미(Lissorhoptrus oryzophilus )에 대한 성충 전용살충제 Clothianidin 액상수화제의 살충제 감수성 시험을 실시한 결과, 비타민A 강화벼에서 72시간-$LC_{50}$은 유효농도 0.018mg/L (95% 신뢰한계는 0.013~0.023mg/L)이었으며, Bt벼의 모본으로 대조로 사용한 낙동벼의 72시간-$LC_{50}$은 유효농도 0.021mg/L(95% 신뢰한계는 0.016~0.026mg/L)이었다. 72시간-$LC_{50}$은 낙동벼에서 다소 높았지만, 해충저항성 Bt벼 72시간-$LC_{50}$이 낙동벼의 95% 신뢰한계 내에 포함되어, 두 품종의 $LC_{50}$값에 유의성이 없는 것으로 판단된다.

Keywords

References

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