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Requirement of CD4 Help for Induction of CD8 T Cell Response Specific for Virally Derived H60

  • Ryu, Su-Jeong (Department of Biomedical Sciences, Seoul National University College of Medicine) ;
  • Kang, Bo-Ra (Department of Biomedical Sciences, Seoul National University College of Medicine) ;
  • Kim, Seok-Ho (Therapeutic Antibody Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Tae-Woo (Graduate School of Medicine, Korea University) ;
  • Chang, Jun (Division of Life and Pharmaceutical Sciences, Ewha Woman's University) ;
  • Choi, Eun-Young (Department of Biomedical Sciences, Seoul National University College of Medicine)
  • Received : 2012.05.09
  • Accepted : 2012.05.29
  • Published : 2012.06.30
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Abstract

CD40-CD40L-mediated help from CD4 T cells is essential to induce primary CD8 T cell responses specific to the non-inflammatory cell-based antigen H60. In this study, using H60 as a model antigen, we generated recombinant vaccinia viruses (rVVs) expressing the H60 CD8 epitope and investigated whether CD4 help was required to activate the CD8 T cell response specific to the virally expressed H60. The immune response after infection with rVVs expressing H60 was similar to that after immunization with H60 congenic splenocytes, with a peak frequency of H60-specific CD8 T cells detected in the blood on day 10 post-infection. A CD8 T cell response specific for virally derived H60 was not induced in CD4-depleted mice, but was in CD40-deficient mice. These results provide insights into the characterization of the CD8 T cell response specifically for antigens originating from cellular sources compared to viral sources.

Keywords

References

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