Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Municipal Wastewater Treatment and Microbial Diversity Analysis of Microalgal Mini Raceway Open Pond

미세조류 옥외 배양시스템을 이용한 도시하수 정화 및 미생물 군집다양성 분석

  • Kang, Zion (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Byung-Hyuk (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Shin, Sang-Yoon (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Oh, Hee-Mock (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Hee-Sik (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology)
  • 강시온 (한국생명공학연구원 환경바이오연구센터) ;
  • 김병혁 (한국생명공학연구원 환경바이오연구센터) ;
  • 신상윤 (한국생명공학연구원 환경바이오연구센터) ;
  • 오희목 (한국생명공학연구원 환경바이오연구센터) ;
  • 김희식 (한국생명공학연구원 환경바이오연구센터)
  • Received : 2012.08.29
  • Accepted : 2012.09.20
  • Published : 2012.09.30
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Abstract

Microalgal biotechnology has gained prominence because of the ability of microalgae to produce value-added products including biodiesel through photosynthesis. However, carbon and nutrient source is often a limiting factor for microalgal growth leading to higher input costs for sufficient biomass production. Use of municipal wastewater as a low cost alternative to grow microalgae as well as to treat the same has been demonstrated in this study using mini raceway open ponds. Municipal wastewater was collected after primary treatment and microalgae indigenous in the wastewater were encouraged to grow in open raceways under optimum conditions. The mean removal efficiencies of TN, TP, COD-$_{Mn}$, $NH_3$-N after 6 days of retention time was 80.18%, 63.56%, 76.34%, and 96.74% respectively. The 18S rRNA gene analysis of the community revealed the presence of Chlorella vulgaris and Scenedesmus obliquus as the dominant microalgae. In addition, 16S rRNA gene analysis demonstrated that Rhodobacter, Luteimonas, Porphyrobacter, Agrobacterium, and Thauera were present along with the microalgae. From these results, it is concluded that microalgae could be used to effectively treat municipal wastewater without aerobic treatment, which incurs additional energy costs. In addition, municipal wastewater shall also serve as an excellent carbon and nitrogen source for microalgal growth. Moreover, the microalgal biomass shall be utilized for commercial purposes.

미세조류는 광합성을 통하여 바이오디젤과 같은 부가가치상품을 생산할 수 있으며, 미세조류를 이용한 생명공학 기술이 주목 받고 있다. 그러나 질소원과 탄소원은 미세조류 배양 비용을 높여 충분한 바이오매스 생산에 제한요소가 되고 있다. 미세조류를 배양하는데 도시하수를 이용하는 것은 생산단가를 낮추는 좋은 대안이 될 수 있으며, 본 연구에서는 옥외 수질정화 배양 시스템(mini raceway open pond)을 이용하여 적용했다. 실험에 사용한 도시하수는 하수종말처리장의 1차 침전지를 거친 유입수를 이용하였으며, 토착 미세조류를 mini raceway open pond에서 배양하였다. 체류시간 6일의 운전 후 TN, TP, COD-$_{Mn}$, $NH_3$-N의 평균 제거 효율은 80.18%, 63.56%, 76.34%, 96.74%로 각각 나타났다. 18S rRNA gene 분석결과 녹조류인 Chlorella, Scenedesmus가 우점하였으며, 16S rRNA gene 분석결과 Rhodobacter, Luteimonas, Agrobacterium, Thauera, Porphyrobacte의 5종의 bacteria가 동정되었다. 이러한 결과를 통하여 미세조류를 이용한 호기성 처리나 과도한 발전비용 없이 효과적인 하수처리를 할 수 있는 가능성을 확인하였다. 그리고 도시하수는 미세조류 배양에 필요한 탄소원과 질소원을 제공할 수 있으며 미세조류 바이오매스는 상업적 목적으로 이용될 수 있는 가능성을 확인할 수 있었다.

Keywords

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