Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Isolation and functional characterization of BrUGT gene encoding a UDP-glycosyltransferase from Chinese cabbage (Brassica rapa)

  • Jung, Yu-Jin (Institute of Genetic Engineering, Hankyong National University) ;
  • Lee, Hye-Jung (Department of Crop Science, Chungbuk National University) ;
  • Choi, Jang-Sun (National Institute of Horticultural & Herbal Science) ;
  • Cho, Yong-Gu (Department of Crop Science, Chungbuk National University) ;
  • Nou, Ill-Sup (Department of Horticulture, Sunchon National University) ;
  • Kang, Kwon-Kyoo (Department of Horticulture, Hankyong National University)
  • Received : 2012.09.20
  • Accepted : 2012.09.23
  • Published : 2012.09.30
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Abstract

Glycosyltransferases are enzymes (EC 2.4) that catalyze the transfer of monosaccharide moieties from activated nucleotide sugar to a glycosyl acceptor molecule which can be a carbohydrate, glycoside, oligosaccharide, or a polysaccharide. In this study, a UDP-glucosyltransferase cDNA was isolated from Brassica rapa using a rapid amplification of cDNA ends (RACE) and subsequently named BrUGT. It has a full-length cDNA of 1,236 bp with 119 bp 5'-untranslated region (UTR), a complete ORF of 834 bp encoding a polypeptide of 277 amino acids (31.19 kDa) and a 3'-UTR of 283 bp. BLASTX analysis hits a catalytic domain of Glycos_transf_1 super family (cl12012) that belongs to the Glycosyltransferases group 1 with tetratricopeptide (TPR) regions located between 165 to 350 bp. Expression analysis showed high mRNA transcripts in pistil, followed by petal, seed and calyx of flower. Moreover, expression analysis of BrUGT in Chinese cabbage seedlings under stresses of cold, salt, PEG, $H_2O_2$, drought and ABA showed elevated mRNA transcript. Furthermore, when BrUGT gene was transformed into rice using pUbi-1 promoter, overexpression was evident among the $T_1$ plants. This study provides insights into the function of BrUGT in plants.

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References

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