Indirect Evidence on Sex Reversal with Sex Ratio of Tegillarca granosa (Bivalvia: Arcidae) and Ruditapes philippinarum (Bivalvia: Veneridae)

  • Lee, Jung-Sick (Department of Aqualife Medicine, Chonnam National University) ;
  • Ku, Ka-Yeon (Department of Aqualife Medicine, Chonnam National University) ;
  • Kim, Hye-Jin (Department of Aqualife Medicine, Chonnam National University) ;
  • Park, Ji-Seon (Department of Aqualife Medicine, Chonnam National University) ;
  • Park, Jung-Jun (Aquaculture Management Division, Aquaculture Research Institute, NFRDI) ;
  • Shin, Yun-Kyung (Aquaculture Management Division, Aquaculture Research Institute, NFRDI) ;
  • Jeon, Mi-Ae (Department of Aqualife Medicine, Chonnam National University)
  • Received : 2012.08.09
  • Accepted : 2012.09.21
  • Published : 2012.09.30

Abstract

This study attempts to propose the possibility of the sex reversal in Tegillarca granosa and Ruditapes philippinarum by confirming the changes in the sex ratio with the shell length (SL) in the same population level. For analysis of sex ratio with SL, 1500 individuals of T. granosa (SL 10.1-45.0 mm) and 712 individuals of R. philippinarum (SL 15.1-70.0 mm) were used. Sex was analyzed histologically. The average sex ratios (F:M) of T. granosa and R. philippinarum were 1:1.22 and 1:0.96, respectively. However, sex ratio was found to differ when the clams were divided into groups according to SL in 5.0 mm intervals. Both species displayed the tendency of increase in the proportion of female with increase in SL. In this study, changes in the sex ratio in accordance with the growth of T. granosa and R. philippinarum are determined to be indirect evidence that signifies their sex reversal.

Keywords

Acknowledgement

Supported by : National Research Foundation of Korea (NRF)

References

  1. Asif M (1979) Hermaphroditism and sex reversal in the four common oviparous species of oysters from the coast of Karachi. Hydrobiologia 66:49-55. https://doi.org/10.1007/BF00019139
  2. Behzadi S, Parivar K, Roustaian P (1997) Gonadal cycle of pearl oyster, Pinctada fucata (Gould) in northeast Persian Gulf, Iran. J Shellfish Res 16:129-135.
  3. Britton AC, Morton B (1982) A dissection guide, field and laboratory manual for the introduced bivalve Corbicula fluminea. Malacol Rev Suppl 3:1-82.
  4. Chang YJ, Lee TY (1982) Gametogenesis and reproductive cycle of the cockle, Fulvia mutica (Reeve). Bull Kor Fish Soc 15:241-253.
  5. Chung EY, Ryou DK, Lee JH (1994) Gonadal development, age and growth of the shortnecked clam, Ruditapes philippinarum (Pelecypoda: Veneridae), on the coast of Kimje, Korea. Korean J Malacol 10:38-54.
  6. Coe WR (1934) Alternation of sexuality in oysters. Amer Nat 68:236-251. https://doi.org/10.1086/280542
  7. Coe WR (1943) Sexual differentiation in mollusks. I. Pelecypods. Quart Rev Biol 18:154-164. https://doi.org/10.1086/394673
  8. Drury RAB, Wallington EA (1980) Carleton's Histological Technique, p520, Oxford, Oxford University Press.
  9. Eversole AG (2001) Reproduction in Mercenaria mercenaria. In: Kraeuter JN, Castagna M, eds. Biology of the Hard Clam, p 221-260, New York, Elsevier.
  10. Gagne F, Blaise C, Pellerin J, Pelletier E, Strand J (2005) Health status of Mya arenaria bivalves collected from contaminated sites in Canada (Saguenay Fjord) and Denmark (Odense Fjord) during their reproductive period. Ecotoxicol Environ Saf 64:348-361.
  11. Galtsoff PS (1964) The American oyster Crassostrea virginica Gmelin. Fish Bull 64:1-480.
  12. Garcia-Dominguez F, Garcia-Gasca SA, Castro-Ortiz JL (1994) Spawning cycle of the red clam, Megapitaria aurantiaca (Sowerby, 1831) (Veneridae) at Isla Espiritu Santo, Baja California Sur, Mexico. J Shellfish Res 13:417-423.
  13. Gosling E (2004) Bivalve Molluscs: Biology, Ecology and Culture. P443, Oxford, Blackwell Science.
  14. Guo X, Hedgecock D, Hershberger WK, Cooper K, Allen SK Jr (1998) Genetic determinants of protandric sex in the Pacific oyster, Crassostrea gigas Thunberg. Evolution 52:394-402. https://doi.org/10.2307/2411076
  15. Heard WH (1979) Hermaphroditism in Elliptio (Pelecypoda: Unionidae). Malacol Rev 12:21-28.
  16. Heller J (1993) Hermaphroditism in molluscs. Biol J Linne Soc 48:19-42. https://doi.org/10.1111/j.1095-8312.1993.tb00874.x
  17. Ju SM, Park JJ, Lee JS (2009) Induction of intersex and masculinization of the equilateral venus, Gomphina veneriformis (Bivalvia: Veneridae) by zinc. Anim Cells and Syst 13:339-344. https://doi.org/10.1080/19768354.2009.9647227
  18. Lee JH (1997) Studies on the gonadal development and gametogenesis of the granulated ark, Tegillarca granosa (Linne). Kor J Malacol 13:55-64.
  19. Lee JS, Cho HS, Jin YG, Park JJ, Shin YK (2009) Reproductive disrupting effect of organotin compound in the ark shell, Scapharca broughtonii (Bivalvia: Arcidae). Anim Cells and Syst 13:223-227. https://doi.org/10.1080/19768354.2009.9647214
  20. Lee JS, Park JJ (2007) Risk assessment of nonylphenol using the sex ratio, sexual maturation, intersex and lipofuscin accumulation of the equilateral venus, Gomphina veneriformis (Bivalvia: Veneridae). J Kor Fish Soc 40: 16-23.
  21. Mackie GL (1984) Bivalves. In: Tompa AS, Verndonk NH, Biggelaar JAM, eds. The Mollusca, Reproduction, vol. 7. p 351-418, New York, Academic Press.
  22. Menzel W (1989) The biology, fishery and culture of quahog clam, Mercenaria. In: Manzi JJ, Castagna M, eds. Clam Culture in North America, p201-242, Amsterdam, Elsevier Science Publishers.
  23. Orton JH (1933) Observations and experiments on sexchange in the European oyster (O. edulis). Part III. On the fate of unspawned ova. Part IV. On the change from male to female. J Mar Biol Assn 6:1-54.
  24. Osanai K (1975) Seasonal gonad development and sex alteration in the scallop Patinopecten yessoensis. Bull Mar Biol Stn Asamushi 15:81-88.
  25. Park JJ, Lee JY, Lee JS, Chang YJ (2003) Gonadal development and gametogenic cycle of the equilateral venus, Gomphina veneriformis (Bivalvia: Veneridae). J Kor Fish Soc 36:352-357.
  26. Sastry AN (1979) Pelecypoda (excluding Ostreidae). In:Giese AG, Pearse PJ, eds. Reproduction of Marine Invertebrates, vol. 5. p113-292, New York, Academic Press.
  27. Thompson RJ, Newell RIE, Kennedy VS, Mann R (1996) Reproductive process and early development. In: Kennedy VS, Newell RIE, Eble AF, eds. The Eastern Oyster Crassostrea virginica, p335-70, College Park, Maryland, Maryland Sea Grant.
  28. Van Der Schalie H, Locke F (1941) Hermaphroditism in Anodonta grandis a freshwater mussel. Science 432: 1-7.
  29. Ventilla RF (1982) The scallop industry in Japan. Adv Mar Biol 20:310-382.
  30. Villalejo-Fuerte M, Garcia-Dominguez F (1998) Reproductive cycle of Spondylus leucacanthus (Broderip, 1833) (Bivalvia: Spondylidae) at Isla Danzante, Gulf California. J Shellfish Res 17:1037-1042.
  31. Walne PR (1974) Culture of Bivalve Molluscs: 50 years Experience at Conwy, P173, Surrey, Fishing News (Books) Ltd.
  32. Wi CH, Chang YJ, Lee SJ, Hur YB, Lee JS (2003) Sexual maturation and gametogenic cycle of the hard shelled mussel, Mytilus coruscus (Bivalvia: Mytilidae). J Aquaculture 16:245-251.
  33. Yusa Y (2007) Causes of variation in sex ratio and modes of sex determination in the Mollusca - an overview. Amer Malac Bull 23:89-98. https://doi.org/10.4003/0740-2783-23.1.89