Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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In vitro Test of Mycelial Growth Inhibition of 5 Fungi Pathogenic to Strawberries by Ultraviolet-C (UV-C) Irradiation

자외선(UV-C) 조사에 의한 딸기병원균의 균사생장억제

  • Kim, Seon Ae (Department of Horticulture & Life Science and LED-IT Fusion Technology Research Center, Yeungnam University) ;
  • Ahn, Soon-Young (Department of Horticulture & Life Science and LED-IT Fusion Technology Research Center, Yeungnam University) ;
  • Oh, Wook (Department of Horticulture & Life Science and LED-IT Fusion Technology Research Center, Yeungnam University) ;
  • Yun, Hae Keun (Department of Horticulture & Life Science and LED-IT Fusion Technology Research Center, Yeungnam University)
  • 김선애 (영남대학교 원예생명과학과 및 LED-IT산업화융합센터) ;
  • 안순영 (영남대학교 원예생명과학과 및 LED-IT산업화융합센터) ;
  • 오욱 (영남대학교 원예생명과학과 및 LED-IT산업화융합센터) ;
  • 윤해근 (영남대학교 원예생명과학과 및 LED-IT산업화융합센터)
  • Received : 2012.06.05
  • Accepted : 2012.08.13
  • Published : 2012.10.31
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Abstract

In strawberry production, among others, the high incidence of diseases by pathogenic fungi resulting in the reduction of fruit yield and quality requires the development of eco-friendly management systems rather than chemical sprays to control them. The diameter of colonies grown in media at $25^{\circ}C$ for 5 days was measured to evaluate the in vitro inhibition of mycelial growth of 5 pathogenic fungi by irradiation with ultraviolet (UV-C, 264 nm). The mycelial growth of 5 pathogenic fungi was inhibited in potato dextrose agar (PDA) by the irradiation of UV-C for 1 hour a day, and was dramatically inhibited by the irradiation of UV-C for 9-12 h a day. The irradiation of UV-C for 9-12 h a day inhibited completely the growth of the late blight pathogen, Phytophthora cactorum. The irradiation distance of 40 to 50 cm was effective for the inhibition of mycelial growth of fungi. The mycelial growth of fungi without pre-incubation was inhibited strongly by UV-C irradiation compared to fungi pre-incubated for 2 days without light. The mycelia growth of Colletotrichum gloeosprioides and Fusarium oxysporum was inhibited strongly by UV-C irradiation in vegetable 8 juice agar compared to PDA.

딸기병해는 생육기 동안 과실의 생산량감소 및 품질저하를 가져오고 있으며, 약제방제의 어려움 등으로 인해 친환경적인 병해 방제법의 개발이 요구된다. 실내에서 자외선(UV-C, 264 nm)조사에 의한 딸기병원균의 균사생장 억제효과를 구명하기 위하여 5종의 딸기병원균을 자외선을 조사하면서 $25^{\circ}C$ 항온실에서 배양한 후 균총의 직경을 측정하였다. 5종의 딸기병원균을 PDA 상에서 배양하면서 자외선을 조사하면, 하루 1시간 조사에 의해서도 생장이 억제되었고 9-12시간을 조사한 균의 생육은 현저하게 억제되었으며 역병균은 생육이 완전하게 억제되었다. 자외선광원과 병원균의 거리에서는 40-50 cm의 거리에서 자외선을 조사한 배양기에서 병원균생장의 억제효과가 크게 나타났다. 배지에 접종한 후 바로 자외선을 처리한 병원균은 모두 2일간 배양한 후 자외선을 접종한 배양기에서보다 생육이 억제되었다. 딸기 탄저병균(C. gloeosprioides)과 시들음병균(F. oxysporum)은 V8A에서 자외선에 의한 균의 생장억제효과가 큰 것으로 나타났다.

Keywords

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