건조방법에 따른 꾸지뽕열매 추출물의 항산화활성과 Angiotensin Converting Enzyme I 저해활성

Antioxidant and Angiotensin Converting Enzyme I Inhibitory Activities of Extracts from Mulberry (Cudrania tricuspidata) Fruit subjected to Different Drying Methods

  • 윤광섭 (대구가톨릭대학교 식품가공학전공) ;
  • 김재원 (대구가톨릭대학교 식품가공학전공)
  • Youn, Kwang-Sup (Dept. of Food Science and Technology, Catholic University of Daegu) ;
  • Kim, Jae-Won (Dept. of Food Science and Technology, Catholic University of Daegu)
  • 투고 : 2012.06.20
  • 심사 : 2012.07.16
  • 발행 : 2012.10.31


건조 꾸지뽕나무 열매의 기능성 소재으로서의 개발 가능성을 알아보고자 70% ethanol 추출물에 대한 생리활성을 측정하였다. 실험군은 꾸지뽕나무 열매 동결건조분말 70% ethanol 추출물(ECFD), 열풍건조분말 70% ethanol 추출물(ECHD), 적외선건조분말 70% ethanol 추출물(ECID)을 각각 동결건조하여 실험에 사용하였다. 수율은 적외선건조에서 높은 수율을 나타내었다. 색도는 $L^*$, $a^*$, $b^*$$H^{\circ}$ 값 모두 열풍건조 및 적외선건조에서 감소하였으며, 총 폴리페놀 및 플라보노이드 함량은 적외선건조에서 가장 높은 함량을 나타내었다. 전자공여능은 적외선건조에서 높은 활성을 나타내었으며, 환원력 및 ABTS 라디칼 소거활성에서도 유사한 경향을 나타내었다. 아질산염 소거능은 적외선건조, 열풍건조, 동결건조 순으로 높은 활성을 나타내었다. SOD 유사활성은 동결건조에서는 611.97 mUnit, 열풍건조에서는 723.02 mUnit, 적외선건조에서는 891.93 mUnit로 열풍건조 및 적외선건조가 동결건조에 비하여 각각 18.15% 및 45.75% 높은 활성을 나타내었다. 반면 ferrous ion chelating 효과에서는 동결건조(52.36%)>적외선건조(47.16%)>열풍건조(30.04%)의 순으로 동결건조 처리구에서 높은 활성을 나타내었다. ACE 저해활성에서는 열풍건조 및 적외선건조가 동결건조에 비하여 각각 9.30% 및 30.88%가 증가하였으며, XO 저해 활성에서는 열풍건조 및 적외선건조가 동결건조에 비하여 각각 5.09% 및 9.02%가 증가하였다. 이상의 결과 적외선 건조 처리한 꾸지뽕나무 열매 추출물은 다양한 항산화 활성 증대효과를 나타냄으로써 산화적 손상 예방 소재개발에 활용가치가 있다고 사료된다.


연구 과제 주관 기관 : 대구가톨릭대학교


  1. Johnston RB Jr, Keele BB Jr, Misra HP, Lehmeyer JE, Webb LS, Baehner RL, RaJagopalan KV. 1975. The role of superoxide anion generation in phagcytic bactericidal activity. Studies with normal and chronic granulomatous disease leukocyte. J Clin Invest 55: 1357-1372.
  2. Danrong Z, Yuquiong C, Dejiang N. 2009. Effect of water quality on the nutritional components and antioxidant activity of green tea extracts. Food Chem 113: 110-114.
  3. Jang IM. 2003. Treatise on Asian Herbal Medicines. Natural Products Science Seoul National University Press, Seoul, Korea.
  4. Ottersen T, Vance B, Doorenbos NJ, Chang BL, el-Feraly FS. 1997. The crystal structure of cudranone, 2,6,3'-trihydroxy- 4-methoxy-2'-(3-methyl-2-butenyl)-benzophenone: a new antimicrobial agent from Cudrania chochinchinensis. Acta Chem Scand B 31: 434-436.
  5. Chen F, Nakashima N, Kimura I, Kimura M. 1995. Hypoglycemic activity and mechanisms of extracts from mulberry leaves (Folium mori) and cortex mori radicis in streptozotocin- induced diabetic mice. Yakugaku Zasshi 115: 476-482.
  6. Cha JY, Kim HJ, Jun BS, Cho YS. 2000. Effects of waterextract of leaves from Morus alba and Cudrania tricuspidata on the lipid concentration of serum and liver in rats. J Korean Soc Agric Chem Biotechnol 43: 303-308.
  7. Kang DG, Hur TY, Lee GM, Oh HC, Kwon TO, Sohn EJ, Lee HS. 2002. Effects of Cudrania tricuspidata water extract on blood pressure and renal functions in NO-dependent hypertension. Life Sci 70: 2599-2609.
  8. Lee IK, Kim CJ, Song KS, Kim HM, Koshino H, Uramoto M, Yoo ID. 1996. Cytotoxic benzyl dihydroflavonols from Cudrania tricuspidata. Phytochemistry 41: 213-216.
  9. Chon IJ, Lee SW, Cha JH, Han JH, Whang WK. 2005. Antioxidant compounds of Cudrania tricuspidata leaves. Yakhak Hoeji 49: 416-421.
  10. Lee IK, Song KS, Kim SJ, Kim HM, Oh GT, Yoo ID. 1994. Tumor cell growth inhibition and antioxidative activity of flavonoids from the stem bark of Cudrania tricuspidata. Agric Chem Biotechnol 37: 105-109.
  11. Lee SJ. 2002. Antioxidative activity of flavonoid compounds from Cudrania tricuspidata root bark. MS Thesis. Chung-Ang University, Seoul, Korea.
  12. Han XH, Hong SS, Hwang JS, Jeong SH, Hwang JH, Lee MH, Lee MK, Lee DH, Ro JS, Hwang BY. 2005. Monoamine oxidase inhibitory constituents from the fruits of Cudrania tricuspidata. Arch Pharm Res 28: 1324-1327.
  13. Dewanto V, Wu X, Adom KK, Liu RH. 2002. Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. J Agric Food Chem 50: 3010-3014.
  14. Abdel-Hameed ESS. 2008. Total phenolic contents and free radical scavenging activity of certain Egyptian Ficus species leaf samples. Food Chem 114: 1271-1277.
  15. Blois MS. 1958. Antioxidant determination by the use of a stable free radical. Nature 181: 1199-1200.
  16. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radial cation decolorization assay. Free Radic Biol Med 26: 1231-1237.
  17. Arabshahi-Delouee S, Urooj A. 2007. Antioxidant properties of various solvent extracts of mulberry (Morus indica L.) leaves. Food Chem 102: 1233-1240.
  18. Kato H, Lee IE, Chuyen N, Kim SB, Hayase F. 1987. Inhibition of nitrosamine formation by nondialyzable melanoidins. Agric Biol Chem 51: 1333-1338.
  19. Yen GC, Duh PD, Tsai HL. 2002. Antioxidant and pro-oxidant properties of ascorbic acid and garlic acid. Food Chem 79: 307-313.
  20. Martin JP Jr, Dailey M, Sugarman E. 1987. Negative and positive assay of superoxide dismutase based on hematoxylin autoxidation. Arch Biochem Biophys 255: 329-336.
  21. Cushman DW, Cheung HS. 1971. Spectrophotometric assay and properties of the angiotensin-converting enzyme of rabbit lung. Biochem Pharmacol 20: 1637-1648.
  22. Ozer N, Müftüoglu M, Ataman D, Ercan A, Ogüs IH. 1999. Simple, high-yield purification of xanthine oxidase from bovine milk. J Biochem Biophys Methods 39: 153-159.
  23. Chae SI, Kim BJ. 1995. Statistical analysis for SPSS/PC. Bubmoon Publishing Co., Seoul, Korea, p 66-75.
  24. Cha JY, Jeong JJ, Kim YT, Seo WS, Yang HJ, Kim JS, Lee YS. 2006. Detection of chemical characteristics in Hamcho (Salicornia herbacea) according to harvest periods. J Life Sci 16: 683-690.
  25. Woo KS, Hwang IG, Noh YH, Jeong HS. 2007. Antioxidant activity of heated licorice (Glycyrrhiza uralensis Fisch) extracts in Korean. J Korean Soc Food Sci Nutr 36: 689-695.
  26. Oh PS, Lee HJ, Lim KT. 2009. Inhibitory effect of glycoprotein isolated from Cudrania tricuspidata bureau on histamine release and COX-2 activity in RBL-2H3 cells. Korean J Food Sci Technol 41: 405-412.
  27. Kang HK, Yoo YK, Lee SK. 2003. Effects of prestorage heat treatment on changes of phenolic compound contents and incidence of skin blackening in 'Niitaka' pear fruits during cold storage. J Kor Soc Hort Sci 44: 197-200.
  28. Kim JH, Kwak DY, Choi MS, Moon KD. 1999. Comparison of the chemical compositions of Korean and Chinese safflower (Carthamus tinctororius L.) seed. Korean J Food Sci Technol 31: 912-918.
  29. Johnson JE, Walford R, Harma D, Miquel J. 1986. Free radicals, aging and degenerative disease. Alen R, ed. Liss, New York, NY, USA. p 325-371.
  30. Lee KS, Kim GH, Kim HH, Kim ES, Park HM, Oh MJ. 2008. Quality characteristics of tea thermally processed from dried Ixeris dentata root. Korean J Food Preserv 15: 524-531.
  31. Kim OK, Ho JN, Nam DE, Jun WJ, Hwang KT, Kang JE, Chae OS, Lee JM. 2012. Hepatoprotective effect on Curdrania tricuspidata extracts against oxidative damage. J Korean Soc Food Sci Nutr 41: 7-13.
  32. Yen GC. 1990. Influence of seed roasting process on the changes in composition and quality of sesame (Sesame indicum) oil. J Sci Food Agric 50: 563-570.
  33. Park NY, Jeong YJ, Lee GD, Kwon JH. 2000. Monitoring of maillard reaction characteristics under various roasting conditions of Polygonatum odoratum root. J Korean Soc Food Sci Nutr 29: 647-654.
  34. Nice DJ, Robinson DS, Holden MA. 1995. Characterisation of a heat-stable antioxidant co-purified with the superoxide dismutase activity from dried peas. Food Chem 52: 393-397.
  35. Seo SJ, Choi Y, Lee SM, Kong S, Lee J. 2008. Antioxidant activities and antioxidant compounds of some specialty rices. J Korean Soc Food Sci Nutr 37: 129-135.
  36. Funayama S, Hikino H. 1979. Hypotensive principles of Diospyros kaki leaves. Chem Pharm Bull (Tokyo) 27: 2865-2868.
  37. Hatano T, Yasuhara T, Yoshihara R, Okuda T, Ikegami Y, Matsuda1 M, Yazaki1 K, Agata I, Nishibe S, Noro T, Yoshizaki M, Okuda T. 1991. Inhibitory effects of galloylated flavonoids on xanthine oxidase. Planta Med 57: 83-84.

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