Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Genetic Diversity of Endophytic Fungal Strains Isolated from the Roots of Coastal Plants in Ulleung Island for Restoration of Coastal Ecosystem

해안 생태계 복원을 위한 울릉도에 자생하는 해안식물의 뿌리로부터 분리된 내생진균류의 유전적 다양성 분석

  • Kim, Miae (School of Life Sciences and Biotechnology, Kyungpook National University) ;
  • You, Young-Hyun (School of Life Sciences and Biotechnology, Kyungpook National University) ;
  • Yoon, Hyeokjun (School of Life Sciences and Biotechnology, Kyungpook National University) ;
  • Kim, Hyun (School of Life Sciences and Biotechnology, Kyungpook National University) ;
  • Seo, Yeonggyo (School of Life Sciences and Biotechnology, Kyungpook National University) ;
  • Khalmuratova, Irina (School of Life Sciences and Biotechnology, Kyungpook National University) ;
  • Shin, Jae-Ho (School of Applied Biosciences, Kyungpook National University) ;
  • Lee, In-Jung (School of Applied Biosciences, Kyungpook National University) ;
  • Choo, Yeon-Sik (Department of Biology, College of National Sciences, Kyungpook National University) ;
  • Kim, Jong-Guk (School of Life Sciences and Biotechnology, Kyungpook National University)
  • Received : 2012.08.21
  • Accepted : 2012.10.29
  • Published : 2012.10.30
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Abstract

Five coastal plant species, Artemisia fukudo, Aster sphathulifolius, Plantago camtschatica, Sedum oryzifolium, and Setaria viridis, were collected from the coastal region of Ulleung Island (Ulleung-Do, South Korea). Thirty-six endophytic fungi were isolated from the roots of these plants, and all were identified by using PCR with the following specifications: internal transcribed spacer 1 (ITS1), 5.8S rRNA, and ITS2 regions. Phylogenetic analysis indicated that all fungal strains belong to the phylum Ascomycota and comprise four orders (Capnodiales, Eurotiales, Hypocreales, and Pleosporales). Among all the identified species, the Eurotiales species were more abundant than species in the other orders. Nine different genera (Alternaria, Aspergillus, Cladosporium, Exserohilum, Fusarium, Neosartorya, Penicillium, Phoma, and Pyrenochaeta) in the four orders were confirmed. Penicillium and Aspergillus species were the most dominant species among the endophytic fungi isolated from the coastal plants. Shannon's diversity index (H') ranged from 0.684 to 1.609, and the endophytic fungi in S. oryzifolium was more diverse compared to the endophytic fungi in the other plants.

본 연구에 사용된 해안식물은 울릉도의 해안지역으로부터 채집하였다. 그리고 식물은 큰비쑥, 해국, 갯질경이, 땅채송화, 갯강아지풀 등 5종류의 식물이 채집되었다. 울릉도에서 채집된 해안식물의 뿌리로부터 36주의 내생진균이 분리되었다. 모든 내생진균류는 ITS영역에 의해 분석 및 동정되었다. 그리고 계통분석 결과, 분리된 내생진균류는 모두 자낭균문에 속하고 4종류의 목(Capnodiales, Eurotiales, Hypocreales and Pleosporales)으로 분류되었으며, 이 중 Eurotiales 목이 가장 분포 비율이 높은 것으로 확인되었다. 그리고 내생진균류를 속으로 분류하였을 때, 9종류의 속(Alternaria, Aspergillus, Cladosporium, Exserohilum, Fusarium, Neosartorya, Penicillium, Phoma and Pyrenochaeta)으로 분류되었으며, Penicillium 속과 Aspergillus 속이 가장 우점종으로 확인되었다. 그리고 Shannon's diversity index (H')는 0.684에서 1.609의 분포로 나타났으며, 땅채송화에서 분리된 내생진균류의 다양성이 다른 식물에 비하여 높은 것으로 확인되었다.

Keywords

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