Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
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The Mechanism for Analgesic Effects of Electroacupuncture on Surgical Ankle Sprain Model Classified as Grade 3 in Rats

수술적 방법으로 유도된 3단계 발목염좌에 대한 전침의 진통기전 연구

  • Yang, Seung-Bum (Department of Meridian & Acupoint, College of Korean Medicine, Wonkwang University) ;
  • Choi, Suck-Jun (Department of Medical Non-commissioned Officer, Wonkwang Health Science University) ;
  • Lee, Sung-Ho (Department of Sports and Leisure Studies, Yewon Arts University) ;
  • Kim, Min-Su (Department of Meridian & Acupoint, College of Korean Medicine, Wonkwang University) ;
  • Sohn, In-Chul (Department of Meridian & Acupoint, College of Korean Medicine, Wonkwang University) ;
  • Kim, Jae-Hyo (Department of Meridian & Acupoint, College of Korean Medicine, Wonkwang University)
  • 양승범 (원광대학교 한의과대학 경혈학교실) ;
  • 최석준 (원광보건대학교 의무부사관과) ;
  • 이성호 (예원예술대학교 생활체육과) ;
  • 김민수 (원광대학교 한의과대학 경혈학교실) ;
  • 손인철 (원광대학교 한의과대학 경혈학교실) ;
  • 김재효 (원광대학교 한의과대학 경혈학교실)
  • Received : 2013.10.31
  • Accepted : 2013.12.02
  • Published : 2013.12.27
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Abstract

Objectives : Electroacupuncture(EA) has been used effectively in producing analgesia on ankle sprain pain of humans and animals. Currently to examine the underlying mechanisms of the EA-induced analgesia, the effects of EA on weight-bearing forces(WBR) were examined at ankle sprain classified as grade 3 in rats. Methods : The severe ankle sprain classified as grade 3 was induced surgically by ankle ligament injury in the Sprague-Dawley rats. WBR of the affected foot were examined to evaluate effects and mechanism of EA(2 Hz, 1 ms pulse width, 2 mA intensity, for 15 min) which was applied to either SI6, GB34, or GB39 acupoints. The rats were pretreated with naltrexone(10 mg/kg, i.p.) as an opioid receptor antagonist or phentolamine(5 mg/kg, i.p) as an ${\alpha}$-adrenoceptor antagonist at 30 min before EA. Results : The daily repeat EA at either SI6, GB34, or GB39 showed significant analgesic effects on the severe ankle sprain. Particularly, daily EA at GB34 showed more potent analgesic effect than the others. In addition, the naltrexone pretreatment completely blocked the analgesic effect of EA at GB34, indicating the involvement of the endogenous opioid system in mediating the effect of EA at GB34. However, the phentolamine pretreament blocked analgesic effects of EA at either SI6 or GB39, indicating the involvement of ${\alpha}$-adrenoceptors in mediating the effect of EA at either SI6 or GB39. Conclusions : These data suggest that EA-induced analgesia on ankle sprain pain is mediated through either endogenous opioids or ${\alpha}$-adrenoceptors dependant on acupoint specific pattern.

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References

  1. Puffer JC. The sprained ankle. Clin Cornerstone 2001 ; 3 : 38-49. https://doi.org/10.1016/S1098-3597(01)90068-7
  2. Wolfe MW, Uhl TL, Mattacola CG, McCluskey LC. Management of ankle sprains. Am Fam Physician. 2001 ; 63(1) : 93–104.
  3. Hanuri Editorial Department. The Merck Manual. Seoul : Hanuri publishing. 2002 : 526-7.
  4. Han KJ, Oh MS, Choi SH. Clinical Research on Effect Technique of Aurucular Acupuncture Therapy on Ankle sprain. Daejeon University Institute of Oriental Medicine's Collection of Dissertations. 2004 ; 13(1) : 287-93.
  5. Kim KY, Woo KH. The Effects of Alphabet Exercise with SSP Electrotherapy on Lateral Ankle Sprain. The Journal of Korean Society of Physical Therapy. 2004 ; 16(2) : 323-31.
  6. Hwang JS, Lim DJ, Hwang JH, Cho HS, Kim KH. Study of the Effect of Blood-Letting Cupping Treatment for Acute Ankle Sprain: A Randomize Controlled Trial. Journal of Korean Acupuncture & Moxibustion Medicine Society. 2005 ; 22(3) : 243-52.
  7. Jung HK, Jeong YP, Chiang SY, Lee JE, Wi J, Im JA, et al. The Comparative Study on the Acupuncture at Affected Limb and Unaffected Limb on Treating Ankle Sprain of Acute Stage. Journal of Korean Acupuncture & Moxibustion Medicine Society. 2008 ; 25(3) : 107-13.
  8. Kim KT, An BJ, Kang MS, Song HS. A Clinical Study of Bee Venom Acupuncture Therapy on Chronic Arthritis of Ankle. Journal of Korean Acupuncture & Moxibustion Medicine Society. 2006 ; 23(4) : 21-6.
  9. Wu TS. 43 Cases of Ankle Sprain Treated by Electroacupuncture. Shanghai Journal of Acupuncture and Moxibustion. 1997 ; 16(S1) : 48.
  10. Jin J. 24 Cases of Ankle Sprain Treated by Electroacupuncture. Guangxi Journal of Traditional Chinese Medicine. 1999 ; 22(05) : 38.
  11. Koo ST, Park YI, Lim KS, Chung K, Chung JM. Acupuncture analgesia in a new rat model of ankle sprain pain. Pain 2002 ; 99 : 423-31. https://doi.org/10.1016/S0304-3959(02)00164-1
  12. Park SY, Choi YY, Jeon IS, Koo ST, Kim KS, Sohn IC, Kim JH. Capsaicin pharmacopuncture modulates ankle sprain induced pain in rats. Korean Journal of Acupuncture. 2006 ; 23(2) : 113-23.
  13. Cho HS, Gwak MS, Lee SY, Hahm TS. The effect of simultaneous electroacupuncture on ankle sprain pain in rats. Korean Journal of Anesthesiology. 2007 ; 52(5) : 566-70. https://doi.org/10.4097/kjae.2007.52.5.566
  14. Ahn HJ, Jeong DH, Hwang KS, Yoon KB, Kim TW, Moon JH, et al. The comparative study on the dong-si acupuncture therapy and general acupuncture therapy for the patient with ankle sprain. Journal of Korean Acupuncture & Moxibustion Medicine Society. 2003 ; 20(1) : 120-30.
  15. Diebschlag W, Nocker W, Lehmacher W. Treatment of acute ankle sprains. Comparison of the efficacy and tolerance of 2 indomethacin- gel preparations. Fortschr Med. 1992; 110(6) : 94–8.
  16. Hernandez W, Raja A, Capuano C. Complex regional pain syndrome. J Am Podiatr Med Assoc. 1999 ; 89(10) : 534–9. https://doi.org/10.7547/87507315-89-10-534
  17. Kim HY, Wang JG, Chung KS, Chung JM. A surgical ankle sprain pain model in the rat: Effect of morphine and indomethacin. Neuroscince Letters 2008 ; 442 : 161-4. https://doi.org/10.1016/j.neulet.2008.06.074
  18. Yang SB, Sohn IC, Lee SH, Cho SH, Kim Jh. Comparison study for analgesic effects of electroacupuncture on surgical ankle sprain model classified as grade 3 in rats. Korean Journal of Acupuncture. 2013 ; 30(1) : 47-55. https://doi.org/10.14406/acu.2013.30.1.047
  19. Yang BS, Kim JH, Jang JH, Ahn SH, Kim KS, Sohn IC. A study of antinociception of electroacupuncture to si6 on the acute ankle sprained rats. Korean Journal of Acupuncture. 2008 ; 25(3) : 81-96.
  20. Merdians & Acupoints Compilation Committee of Korean Medical Colleges. Details of Meridians & Acupoints. A Guidebook for College Students. Daejeon : Jongryenamu Publishing Co. 2010 : 318, 506, 512.
  21. Koo ST, Kim SK, Kim EH, Kim JH, Youn DH, Lee BH, et al. Acupuncture point locations for experimental animal studies in rats and mice. Korean Journal of Acupuncture. 2010 ; 27(3) : 67-78.
  22. Kim JH, Kim HY, Chung K, Chung JM. Electroacupuncture reduces the evoked responses of the spinal dorsal horn neurons in ankle-sprained rats. J Neurophysiol. 2011 ; 105 : 2050-7. https://doi.org/10.1152/jn.00853.2010
  23. Kerkhoffs GM, Rowe BH, Assendelft WJ, Kelly KD, Struijs PA, van Dijk CN. Immobilisation for acute ankle sprain. A systematic review. Arch Orthop Trauma Surg. 2001 ; 121(8) : 462-71. https://doi.org/10.1007/s004020100283
  24. Silvert M. Acupuncture wins BMA approval. BMJ. 2000 ; 321(7252) : 11.
  25. Hao S, Takahata O, Iwasaki H. Electroacupuncture potentiates the antinociceptive effect of intrathecal endomorphin-1 in the rat formalin test. Neurosci Lett. 2000 ; 287(1) : 9-12. https://doi.org/10.1016/S0304-3940(00)01155-1
  26. Hsieh CL, Kuo CC, Chen YS, Li TC, Hsieh CT, Lao CJ, et al. Analgesic effect of electric stimulation of peripheral nerves with different electric frequencies using the formalin test. Am J Chin Med. 2000 ; 28(2) : 291-9. https://doi.org/10.1142/S0192415X00000349
  27. Lee JH Beitz AJ. The distribution of brain-stem and spinal cord nuclei associated with different frequencies of electroacupuncture analgesia. Pain. 1993 ; 52(1) : 11-28. https://doi.org/10.1016/0304-3959(93)90109-3
  28. Anandacoomarasamy A, Barnsley L. Long term outcomes of inversion ankle injuries. Br J Sports Med. 2005 ; 39 : e14(discussion). https://doi.org/10.1136/bjsm.2004.011676
  29. Cho HS, Yang MK, Yoo RG, Hahm TS. Effective acupoint of electroacupuncture on ankle-sprained pain in rats. Korean Journal of Anesthesiology. 2006 ; 51(3) : 354-8.
  30. Mayer DJ, Price DD, Rafii A. Antagonism of acupuncture analgesia in man by the narcotic antagonist naloxone. Brain Res. 1977 ; 121(2) : 368-72. https://doi.org/10.1016/0006-8993(77)90161-5
  31. Han JS. Acupuncture: neuropeptide release produced by electrical stimulation of different frequencies. Trends Neurosci. 2003 ; 26(1) : 17-22. https://doi.org/10.1016/S0166-2236(02)00006-1
  32. Millan MJ. Descending control of pain. Prog Neurobiol. 2002 ; 66(6) : 355-474. https://doi.org/10.1016/S0301-0082(02)00009-6
  33. Chapman CR, Benedetti C, Colpitts YH, Gerlach R. Naloxone fails to reverse pain thresholds elevated by acupuncture: acupuncture analgesia reconsidered. Pain. 1983 ; 16(1) : 13-31. https://doi.org/10.1016/0304-3959(83)90082-9
  34. Mayer DJ. Biological mechanisms of acupuncture. Prog Brain Res. 2000 ; 122 : 457-77.
  35. Schaible HG. Spinal mechanisms contributing to joint pain. Novartis Found Symp. 2004 ; 260 : 4-22. https://doi.org/10.1002/0470867639.ch2
  36. Schaible HG, Ebersberger A, Von Banchet GS. Mechanisms of pain in arthritis. Ann N Y Acad Sci. 2002 ; 966 : 343-54. https://doi.org/10.1111/j.1749-6632.2002.tb04234.x
  37. Shin HK, Lee KH, Park DS. $\alpha$2-Adrenoceptors are Implicated in the Electroacupuncture-induced Analgesia of Experimental Chronic Pain. The Journal of Korean Oriental Medicine. 2004 ; 25(3) : 67-77.
  38. Kim JH, Koo ST, Sohn IC. Heterotopic electroacupuncture modulates formalin -induced pain via descending inhibition in the rat. Korean Journal of Acupuncture. 2006; 23(3) : 55-71.
  39. Watkins LR, Mayer DJ. Organization of endogenous opiate and nonopiate pain control systems. Science. 1982 ; 216(4551) : 1185-92. https://doi.org/10.1126/science.6281891

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