Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
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Anti-Inflammatory Effect of Bower Actinidia in LPS-Stimulated RAW264.7 Cells

LPS로 유도된 RAW264.7 염증모델에서 미후등의 항염증효과

  • Kim, Young-Jun (Department of Meridian & Acupoint, College of Oriental Medicine, Dong-Eui University) ;
  • Song, Choon-Ho (Department of Meridian & Acupoint, College of Oriental Medicine, Dong-Eui University)
  • 김영준 (동의대학교 한의과대학 경락경혈학교실) ;
  • 송춘호 (동의대학교 한의과대학 경락경혈학교실)
  • Received : 2013.11.19
  • Accepted : 2013.12.14
  • Published : 2013.12.27
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Abstract

Objectives : Bower Actinidia has been widely used for treatment of inflammatory diseases, such as jaundice, cystolithiasis. However, the mechanism of its anti-inflammatory activity has not been clarified. In this study, we investigated the inhibitory effect of Bower Actinidia pharmacopuncture extract(BA) on LPS-induced inflammation. Methods : The effect of BA was analyzed by ELISA, RT-PCR and Western blotting in LPS-stimulated RAW264.7 cells. Results : We found that BA suppressed not only the mRNA expression of pre-inflammatory cytokines, cyclooxygenase-2(COX-2) and inducible nitric oxide synthase(iNOS), but also the phosphorylation of ERK, JNK and p38. Conclusions : These results suggest that BA exerts an anti-inflammatory effect through the regulation of the mitogen-activated protein kinase(MAPK) pathway, thereby decreasing production of pre-inflammatory cytokines.

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References

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