Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Comparative Genetic Characteristics of Korean Ginseng using DNA Markers

분자지표를 이용한 고려인삼의 유전적 특성 비교

  • Shin, Mi Ran (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Jo, Ick Hyun (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Chung, Jong Wook (National Agrobiodiversity Center, NAAS, RDA) ;
  • Kim, Young Chang (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Lee, Seung Ho (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Jang Uk (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Hyun, Dong Yun (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Dong Hwi (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Kee Hong (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Moon, Ji Young (Experiment Research Institute of National Agricultural Products Quality Management Service, MIFAFF) ;
  • Noh, Bong Soo (Department of Food Science and Technology, Seoul Women's University) ;
  • Kang, Sung Taek (Department of Crop Science and Biotechnology, Dankook University) ;
  • Lee, Dong Jin (Department of Crop Science and Biotechnology, Dankook University) ;
  • Bang, Kyong Hwan (Department of Herbal Crop Research, NIHHS, RDA)
  • 신미란 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 조익현 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 정종욱 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 김영창 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 이승호 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김장욱 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 현동윤 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김동휘 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김기홍 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 문지영 (국립농산물품질관리원 시험연구소) ;
  • 노봉수 (서울여자대학교 식품공학과) ;
  • 강성택 (단국대학교 식량생명공학과) ;
  • 이동진 (단국대학교 식량생명공학과) ;
  • 방경환 (농촌진흥청 국립원예특작과학원 인삼특작부)
  • Received : 2013.10.14
  • Accepted : 2013.11.11
  • Published : 2013.12.30
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Abstract

The development of random amplified polymorphic DNA (RAPD) and expressed sequence tag-derived simple sequence repeats (EST-SSRs) provided a useful tool for investigating Korean ginseng genetic diversity. In this study, 18 polymorphic markers (7 RAPD and 11 EST-SSR) selected to assess the genetic diversity in 31 ginseng accessions (11 Korean ginseng cultivars and 20 breeding lines). In RAPD analysis, a total of 53 unique polymorphic bands were obtained from ginseng accessions and number of amplicons ranged from 4 to 11 with a mean of 7.5 bands. Pair-wise genetic similarity coefficient (Nei) among all pairs of ginseng accessions varied from 0.01 to 0.32, with a mean of 0.11. On the basis of the resulting data, the 31 ginseng accessions were grouped into six clusters. As a result of EST-SSR analysis, 11 EST-SSR markers detected polymorphisms among the 31 ginseng accessions and revealed 49 alleles with a mean of 4.45 alleles per primer. The polymorphism information content (PIC) value ranged from 0.06 to 0.31, with an average of 0.198. The 31 ginseng accessions were classified into five groups by cluster analysis based on Nei's genetic distances. Consequently, the results of ginseng-specific RAPD and EST-SSR markers may prove useful for the evaluation of genetic diversity and discrimination of Korean ginseng cultivars and breeding lines.

Keywords

References

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