Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Influence of Essential Oil in 'Shiranuhi' Immature Fruit on Antioxidant and Antimicrobial Activities

부지화 미숙과 에센셜 오일의 항산화 및 항균 활성 효과

  • Kim, Sang Suk (Citrus Research Station, National Institute of Horticultural & Herbal Science, RDA) ;
  • Hyun, Ju Mi (Citrus Research Station, National Institute of Horticultural & Herbal Science, RDA) ;
  • Kim, Kwang Sik (Citrus Research Station, National Institute of Horticultural & Herbal Science, RDA) ;
  • Park, Kyung Jin (Citrus Research Station, National Institute of Horticultural & Herbal Science, RDA) ;
  • Park, Suk Man (Citrus Research Station, National Institute of Horticultural & Herbal Science, RDA) ;
  • Choi, Young Hun (Citrus Research Station, National Institute of Horticultural & Herbal Science, RDA)
  • 김상숙 (농촌진흥청 국립원예특작과학원 감귤시험장) ;
  • 현주미 (농촌진흥청 국립원예특작과학원 감귤시험장) ;
  • 김광식 (농촌진흥청 국립원예특작과학원 감귤시험장) ;
  • 박경진 (농촌진흥청 국립원예특작과학원 감귤시험장) ;
  • 박석만 (농촌진흥청 국립원예특작과학원 감귤시험장) ;
  • 최영훈 (농촌진흥청 국립원예특작과학원 감귤시험장)
  • Received : 2013.09.06
  • Accepted : 2013.12.02
  • Published : 2013.12.30
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Abstract

This study was designed to analyze the chemical composition of essential oil in 'Shiranuhi' immature fruit and to test their biological activities. 'Shiranuhi' immature essential oils (SIEO) were obtained by steam distillation from fruits collected from Jeju Island and were analyzed using gas chromatograph (GC)-flame ionization detectors (FID) and GC-MS. Fourteen components were identified in the essential oil. Limonene (75.21%) and terpineol (8.68%) were the major components in SIEO. Since acne vulgaris is the combined result of a bacterial infection and the inflammatory response to that infection, we examined whether SIEO possessed antibacterial against skin pathogens. As a result, SIEO showed excellent antibacterial activities against drug-susceptible and -resistant Propionibacterium acnes and Staphylococcus epidermidis, which are acne-causing bacteria. In this study, SIEO was examined on DPPH radical scavenging activities, which showed moderate antioxidant activity ($SC_{50}$, $15.36{\mu}L/mL$). In order to determined whether SIEO can be safely applied to human skin, the cytotoxicity effects of SIEO were determined by colorimetric MTT assays in normal human fibroblasts and keratinocyte HaCaT cells. They exhibited low cytotoxicity at $0.5{\mu}L/mL$ in both celllines. Based on these results, we suggest the possibility that essential oil of 'Shiranuhi' maybe considered as an antibacterial and antioxidant agent.

Keywords

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