Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Anti-oxidative Effect of Oyster Hydrolysate on the Serum and Hepatic Homogenate in SD-rats

굴 가수분해물이 SD-Rat의 혈청과 간 균질물에 미치는 항산화 효과

  • Hur, Sung-Ik (Dept. of Seafood Science and Technology/Institute of Marine Industry, Gyeongsang National University) ;
  • Park, Si-Hyang (Sun Marine Biotech Co.) ;
  • Lee, Su-Seon (Sun Marine Biotech Co.) ;
  • Choung, Se Young (Dept. of Hygienic Chemistry, College of Pharmacy, Kyung Hee University) ;
  • Choi, Yeung Joon (Dept. of Seafood Science and Technology/Institute of Marine Industry, Gyeongsang National University)
  • 허성익 (경상대학교 해양식품공학과/해양산업연구소) ;
  • 박시향 (선마린바이오테크) ;
  • 이수선 (선마린바이오테크) ;
  • 정세영 (경희대학교 약학대학) ;
  • 최영준 (경상대학교 해양식품공학과/해양산업연구소)
  • Received : 2013.08.21
  • Accepted : 2013.11.27
  • Published : 2013.12.31
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Abstract

This study is conducted to investigate the antioxidative effect of oyster hydrolysates in the serum and liver of SD-rats through the determination of lipid content, production of free radicals and antioxidant enzyme activities. Two different hydrolysates, Protamex-treated and Neutrase-treated hydrolysate with the cross-linking of protein by transglutaminase (TGPN group) and without (PN group), were fed for 6 weeks. TGPN hydrolysate in serum and liver significantly decreased the total cholesterol in the range of 26.1% to 28.9%, and triglyceride in the liver of up to 6.3%. Superoxide radical in the serum and lipid peroxide radical in the liver were significantly decreased in SD-rats fed 200 mg TGPN hydrolysate. Superoxide dismutase activity was significantly decreased in the liver of SD-rats. These results indicate that TGPN hydrolysate could scavenge the superoxide and hydroxyl radicals, and reduce the superoxide dismutase and catalase activities. The TGPN is also protected the oxidation of protein by the free radicals.

Sprague Dawley계 rat을 이용하여 굴 효소 가수분해물 함유식이가 혈청과 간 균질액에 미치는 항산화 효과를 조사하였다. 식이 그룹은 대조군과 실험군으로써 TGPN 굴 효소 가수분해물 100 mg/kg BW/day 함유 식이를 섭취한 실험군(TGPN-100FG), TGPN 200 mg/kg BW/day 함유 식이를 섭취한 실험군(TGPN-200FG), PN 가수분해물 시료 100 mg/kg BW/day 함유 식이를 섭취한 실험군(PN-100 FG), 200 mg/kg BW/day 함유 식이를 섭취한 실험군(PN-200FG) 5 그룹으로 나누어 6주간 식이를 제공하여 실험하였다. 총 콜레스테롤은 TGPN-200FG 식이군이 혈청과 간 균질액에서 각각 28.9%, 26.1%까지 유의적으로 감소하였으며, 간 균질물의 중성지질은 TGPN-100FG 식이군에서 6.3%까지 유의적으로 감소하였다. 수퍼옥사이드 라디칼은 TGPN-200FG에서 유의적으로 34.7% 감소하였다. 굴 가수분해물 TGPN-200FG는 수퍼옥사이드 및 히드록실 라디칼을 소거함으로써 라디칼 소거에 관여하는 효소의 활성 수준을 감소시키며, 단백질 산화를 억제하는 것으로 나타났다.

Keywords

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