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The Recovery Effects of Joojakwhan - A Traditional Korean Medical Poly-herbal Drug for the Cognitions and Motor Functions in Mild Stroke Rat Model by Using Transient Middle Cerebral Artery Occlusion

주작환(朱雀丸)이 일시적 국소 뇌허혈 유발 백서(白鼠)에 대한 인지 및 운동기능 회복에 미치는 효과

  • Kim, Bo-Eun (Department of Oriental Neuropsychiatry, College of Oriental Medicine, Daegu Haany University) ;
  • Kang, Seok-Bong (Department of Oriental Internal Medicine, College of Oriental Medicine, Daegu Haany University) ;
  • Chung, Dae-Kyoo (Department of Oriental Neuropsychiatry, College of Oriental Medicine, Daegu Haany University)
  • 김보은 (대구한의대학교 부속한방병원 한방신경정신과) ;
  • 강석봉 (대구한의대학교 부속한방병원 내과) ;
  • 정대규 (대구한의대학교 부속한방병원 한방신경정신과)
  • Received : 2013.11.13
  • Accepted : 2013.12.16
  • Published : 2013.12.30

Abstract

Objectives: The object of this study is to observe the cognition and motor function recovery effects of Joojakwhan (JJW), a traditional Korean poly-herbal formula for treating various neuropsychiatric diseases such as dementia, for the mildly stroke rats, with 60 minutes of reperfusion transient middle cerebral artery occlusion (tMCAO). Methods: In the present study, 125, 250 and 500 mg/kg of JJW were orally administered, once per day for 10 continuous days 2 hours after the tMCAO. The body weight changes, infarct sizes under 2% 2, 3, 5-triphenyl tetrazolium chloride (TTC) stain, sensorimotor functions and cognitive motor behavior tests were serially monitored with cerebral caspase-3 and cleaved poly (ADP-ribose) polymerase (PARP)-immunoreactivities and histopathological changes. The effects of tMCAO on sensorimotor functions were evaluated by using of limb placing and body-swing tests, and the cognitive motor behaviors were also observed with water maze tests. Results: From the results of tMCAO, with marked decreases of body weights, disorders of sensorimotor functions increases the limb placing test scores, and decrease the numbers and percentages of body swings to the ipsilateral sides. The cognitive motor behaviors increases the distances and time to reach the escape platform which included the inhibitions of the decreases with repeated trials that were observed with focal cerebral cortex infarct volumes. In addition, the marked increases of the atrophy, numbers of degeneration, caspase-3- and PARP-immunoreactive cells around peri-infarct ipsilateral cerebral cortex were also observed in tMCAO controls when compared with the sham control rats, respectively. Conclusions: The results obtained from this study suggest that oral administrations of JJW indicate obvious cognitions and motor function recoveries of the rats with tMCAO, mild strokes, which are mediated by neuro-protective effects through known antioxidant effects of components.

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