Journal of Environmental Health Sciences (한국환경보건학회지)

Korean Society of Environmental Health (한국환경보건학회)
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Occurrence of Indicator Bacteria and Identification of Total Coliforms Using 16S rRNA Gene in Drinking Spring Water in Seoul

서울 약수터의 지표세균 분포 및 16S rRNA 염기서열을 이용한 총대장균군 동정 및 계통분석

  • Yoon, Tae-Ho (Seoul metropolitan government Institute of Health & Environment) ;
  • Lee, Hyang (Seoul metropolitan government Institute of Health & Environment) ;
  • Choi, Geum-Sook (Seoul metropolitan government Institute of Health & Environment) ;
  • Lee, Seung-Joo (Seoul metropolitan government Institute of Health & Environment) ;
  • Lee, Mok-Young (Seoul metropolitan government Institute of Health & Environment) ;
  • Eo, Soo-Mi (Seoul metropolitan government Institute of Health & Environment)
  • 윤태호 (서울시보건환경연구원) ;
  • 이향 (서울시보건환경연구원) ;
  • 최금숙 (서울시보건환경연구원) ;
  • 이승주 (서울시보건환경연구원) ;
  • 이목영 (서울시보건환경연구원) ;
  • 어수미 (서울시보건환경연구원)
  • Received : 2013.11.19
  • Accepted : 2013.12.27
  • Published : 2013.12.31
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Abstract

Objectives: This study was performed in order to detect indicator bacteria in drinking spring water (DSW) samples in Seoul Metropolitan City, and to identify their genus through 16S rRNA sequencing and then assessing the genetic relation of their strains. Methods: For indicator bacteria detection and identification of total coliforms, we analyzed DSW between the spring and summer seasons. In particular, DSW samples were chosen from sites repeatedly found unsatisfactory in recent years. Results: Heterotrophic plate counts of DSW in the spring and summer season were investigated in the range of 0-550 and 0-800 CFU/mL, respectively. Total coliforms of these were 0-1,900 and 0-2,100 CFU/100mL, fecal coliforms were 0-600 and 0-550 CFU/100mL, and Escherichia coli were 0-7 and 0-326 MPN/100mL. The detection ratio of fecal pollution indicators and that of fecal coliforms increased to 58.6% in the summer from 12.5% in the spring and Escherichia coli increased to 51.4% from 4.7%. As a result of genetic analysis on the isolated bacteria, the genus of total coliforms was classified in the order of Enterobacter spp. 12.7%, Serratia spp. 7.3%, E. hermanii 6.4%, Rahnella spp. 5.5%, Hafnia spp. 4.5%, Escherichia coli 3.6%, Klebsiella spp. 3.6% in the spring season. In the summer season, it was classified in order of Klebsiella spp. 16.6%, Enterobacter spp. 13.0%, Escherichia coli 11.0%, Serratia spp. 8.6%, Raoultella spp. 7.0%, Kluyvera spp. 5.6% and Citrobacter spp. 3.0%. Conclusions: The increase of fecal pollution in summer indicates that special attention to drinking DSW is required.

Keywords

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