한국식품저장유통학회지 (Food Science and Preservation)

  • 제20권5호
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  • Pages.699-704
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  • 2013
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  • 3022-5477(pISSN)
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  • 3022-5485(eISSN)
한국식품저장유통학회 (The Korean Society of Food Preservation)
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Bacillus subtilis HJ18-4를 이용하여 제조한 메밀 속성장의 품질특성

Quality characteristics of buckwheat Soksungjang manufactured by Bacillus subtilis HJ18-4

  • 박나영 (국립농업과학원 농식품자원부) ;
  • 이선영 (국립농업과학원 농식품자원부) ;
  • 김지연 (국립농업과학원 농식품자원부) ;
  • 최혜선 (국립농업과학원 농식품자원부)
  • Park, Na Young (Department of Agro-food Resources, National Academy of Agricultural Science, RDA) ;
  • Lee, Sun Young (Department of Agro-food Resources, National Academy of Agricultural Science, RDA) ;
  • Kim, Ji Yeun (Department of Agro-food Resources, National Academy of Agricultural Science, RDA) ;
  • Choi, Hye Sun (Department of Agro-food Resources, National Academy of Agricultural Science, RDA)
  • 투고 : 2013.06.14
  • 심사 : 2013.11.04
  • 발행 : 2013.10.30
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초록

메밀 속성장은 메밀과 콩으로 제조된 별미장이다. 항균 및 효소활성이 우수한 B. subtilis HJ18-4 균주를 starter로 사용하여 메밀 속성장을 제조하였다. 메밀 속성장 제조과정 중, starter 첨가 시기는 메주 제조단계(Treat-1) 및 염수 첨가단계(Treat-2)로 접종시기를 달리하였다. 발효 중 아미노태질소 함량, 환원당, 효소활성(protease, amylase)의 이화학적 품질특성과 총균 수, 유산균 수 및B. cereus 양 변화 등의 미생물학적 특성을 분석하였다. 그 결과, 총균 수는 발효 15일부터 7~8 log CFU/mL로 증가되었고 아미노태 질소함량은 발효기간 동안 65.38~202.52 mg%로 증가되었다. 반면, 환원당과 효소활성(amylase, protease)은 모든 처리구 에서 감소하였다. 발효기간 중 PlcR 발현에 미치는 영향을 측정한 결과, Treat-1 처리구에서 발효 23일 이후 검출되지 않았다. 메주 제조단계에서 B. subtilis HJ18-4를 접종한 Treat-1이 발효관련 이화학적 특성과B. cereus 저해효과가 우수하였다. 단일 균을 접종하여 제조되는 개량식장 제조 시, 자연발효에 제조된 장의 품질을 재현하기가 매우 어려운 실정이다. 본 연구는 자연발효방법으로 제조되는 메밀 속성장에 항균 및 효소활성이 우수한B. subtilis HJ18-4 균주를 스타터 적용하여 장류 품질개선 방법을 제시하였다.

Buckwheat Soksungjang (BS) is a bealmijang manufactured with buckwheat and soybeans. We manufactured BS using Bacillus subtilis HJ18-4 (HJ18-4), which has high enzyme activities and antibacterial effects. HJ18-4 was inoculated in a different process during the BS manufacturing, which was the meju-making time (Treat 1), and the salt water time was added (Treat 2). The physiochemical and microbial characteristics of the BS were analyzed. As a result, the total aerobic counts (7~8 log CFU/mL) in the BS increased after 15 days of fermentation. Especially, Treat 1 showed higher total aerobic counts and amino-type nitrogen (65.38~202.52 mg%) than Treat 2. During the BS fermentation, the reduction of the sugar contents and the enzyme (protease and amylase) activities decreased. In the relative quantitative expression level of PlcR, Treat 1 did not show toxin gene expressions at the end of the fermentation on Day 23. Treat 1 showed suitable B. cereus physiochemical quality characteristics and inhibition effects. When the modified-form type of fermented soybean paste was manufactured with a single starter, it could not reproduce the natural fermentation quality. These results suggest that the addition of a starter (HJ18-4) in the Meju manufacturing process could enhance the quality characteristics of the manufactured BS via natural fermentation and by suppressing B. cereus.

키워드

참고문헌

  1. Hwang HS (1984) Korean Folk Report. Ministry of Culture, Sports and Tourism (MCST). Seoul, Korea, p 183
  2. Youn SS (1985) Korean Food (History and Cuisine). 4th ed. Suhaksa. Seoul, Korea, p 54-59
  3. Ju JS (1985) Nutrition of soybean. Korea Soybean Digest 2, 16-19
  4. Utsumi S, Gidamis AB, Mikami B, Kito M (1993) Crystallization and preliminary X-ray crystallographic analysis of the soybean proglycinin expressed in Escherichia coli. J Mol Biol, 233, 177-178 https://doi.org/10.1006/jmbi.1993.1495
  5. Duyu kong be T, Ishige T, Takaiwa F (1997) Molecular design of soybean glycinins with enhanced food qualities and development of crops producing such glycinins. Adu Exp Med Biol, 415, 1-15 https://doi.org/10.1007/978-1-4899-1792-8_1
  6. Park JS, Lee TS, Kye HW, Ahn SM, Noh BS (1993) Study on the preparation of kochujang with addition of fruit juices. Korean J Food Sci Technol, 25, 98-104
  7. Choi YS, Ahn C, Shim HH, Choe M, Oh SY, Lee SY (1992) Effect of instant buckwheat noodle on digestibility and lipids profiles of liver and serum in rats. J Korean Soc Food Sci Nutr, 21, 478-483
  8. Lee JS, Maeng YS, Chang YK, Ju JS (1994) Effects of buckwheat on organ weight, glucose and lipid metabolism in streptozotocin induced diabetic rats. Korean J Nutr, 27, 819-827
  9. Yang SH, Choi MR, Kim JK, Chung YG (1992) Optimization of the taste components composition in traditional Korean soybean paste. J Korean Soc Food Nutr, 21, 449-453
  10. Park JS, Lee MY, Kim KS, Lee TS (1994) Volatile flavor components of soybean paste (Doenjang) prepared from different strains. Korean J Food Sci Technol, 26, 255-260
  11. Kim MH, Lee JH (1994) Antioxidant materials in domestic meju and Doenjang. J Korean Soc Food Nutr, 23, 251-260
  12. Kurechi T, Kikuda S, Hasunuma M (1981) Inhibition of Nnitrosamine formation by soya products. Food Cosmet Toxicol, 19, 425-450 https://doi.org/10.1016/0015-6264(81)90445-4
  13. Yu RN, Chung DK, Nam HS, Shin ZI (1996) Effect of soybean hydrolysate on hypertension on spontaneously hypertensive rats. J Korean Soc Food Sci Nutr, 25, 1031-1036
  14. Park KY, Moon SH, Cheigh HS (1996) Antimutagenic effect of Doenjang (Korean soy paste). J Food Sci Nutr, 1, 151-156
  15. Kim BS, Rhee CH, Hong YA, Woo CJ, Jang CM, Kim YB, Park HD (2007) Changes of enzyme activity and physiological functionality of traditional doenjang during fermentation using Bacillus sp. SP-KSW3. Korean J Food Preserv, 14, 545-551
  16. Lee SY, Kim JY, Baek SY, Yeo SH, Koo BS, Park HY, Choi HS (2011) Isolation and identification characteristics of oligotrophic strains with high enzyme activity from buckwheat Sokseongjang. Korean J Food Sci Technol, 43, 735-741 https://doi.org/10.9721/KJFST.2011.43.6.735
  17. Choi HS, Joo SJ, Yoon HS, Kim KS, Song IG, Min KB (2007) Quality characteristics of hwangki (Astragalus membranaceus) cheonggukjang during fermentation. Korean J Food Preserv, 14, 356-363
  18. Kim HJ, Lee JJ, Cheigh MJ, Choi SY (1998) Amylase, protease, peroxidase and ascorbic acid oxidase activity of Kimchi ingredients. Korean J Food Sci Technol, 30, 1333-1338
  19. Lee SY, Park NY, Kim JY, Choi HS (2012) Quality characteristics of rice-doenjang during fermentation by differently shaped meju and adding starter. Korean J Food Nutr, 25, 505-512 https://doi.org/10.9799/ksfan.2012.25.3.505
  20. Dubois M, Gillers KA, Hamilton JK, Rebers PA, Smith F (1956) Colormetric method for determination of sugar and related substance. Anal Chem, 28, 350-352 https://doi.org/10.1021/ac60111a017
  21. Choi HS, Lee SY, Baek SY, Koo BS, Yoon HS, Park HY, Yeo SH (2011) Quality characteristics of buckwheat (Fagopyrum esculentum) Soksungjang. Korean J Food Sci Technol, 43, 77-82 https://doi.org/10.9721/KJFST.2011.43.1.077
  22. Jung SW, Kim YS, Chung KS (1995) Effects of preparation methods and aging temperatures on the properties of rice-doenjang. Agric Chem Biotechnol, 38, 83-89
  23. Hong JS, Park JR, Jeon JR, Cha MH, KJ, Kim JH (2004) Quality characteristics and angiotensin converting enzyme inhibitory activity of Doenjang prepared with Bacillus subtilis SS103. J East Asian Soc Dietary Life, 14, 363-369
  24. Jeong YK, Seo JH, Cho HS (2007) Quality characteristics of Kochujang prepared with commercial protease. Korean J Food Nutr, 4, 378-383
  25. Rhee CH, Lee JB, Jang SM (2000) Change of microorganisms, enzyme activity and physiological functionality in the traditional doenjang with various concentrations of Lentinus edodes during fermentation. J Korean Soc Agric Chem Biotechnol, 43, 277-284
  26. Mok CK, Song KT, Lee JY, Park YS, Lim SB (2005) Changes in microorganisms and enzyme activity of low salt soybean paste (doenjang) during fermentation. Food Eng Prog, 9, 112-117
  27. Kim JY, Lee SY, Park NY, Choi HS (2011) Quality characteristics of black soybean paste (Daemaekjang) prepared with Bacillus subtilis HJ18-4. Korean J Food Sci Technol, 44, 743-749 https://doi.org/10.9721/KJFST.2012.44.6.743
  28. Kwon GH, Lee HA, Kim JH (2010) A bacteriocin of 5-kDa in size Bacillus subtilis 168. Korean J Microbiol Biotechnol, 38, 163-167
  29. Nakamura K, Arakawa K, Kawai Y, Yasuta N, Chujo T, Watanabe M, Iioka H, Tanioka M, Nishimura J, Kitazawa H, Tsurumi K, Saito T (2013) Food preservative potential of gassericin A-containing concentrate prepared from cheese whey culture supernatant of Lactobacillus gasseri LA39. J Anim Sci, 84, 144-149 https://doi.org/10.1111/j.1740-0929.2012.01048.x
  30. Kim DH, Song HP, Sohn JS, Cha BS, Shin MG, Byun MY (2002) Effects of fermentation to improve hygienic quality of powdered raw grains and vegetables raw grains and vegetables using Lactobacillus sp. isolated from Kimchi. J Korean Soc Food Sci Nutr, 31, 765-769 https://doi.org/10.3746/jkfn.2002.31.5.765
  31. Rukure GT (1999) Survival and growth of Bacillus cereus during Gouda cheese manufacturing. Ph D thesis, Pretoria University, South Africa, 43-45

피인용 문헌

  1. Effect of Extracts and Bacteria from Korean Fermented Foods on the Control of Sesame Seed-Borne Fungal Diseases vol.21, pp.4, 2015, https://doi.org/10.5423/RPD.2015.21.4.297
  2. Effect of Korean Fermented Food Extracts and Bacteria Isolated from the Extracts for the Control of Rice Seed-borne Fungal Diseases vol.18, pp.4, 2014, https://doi.org/10.7585/kjps.2014.18.4.383