DOI QR코드

DOI QR Code

Easy production techniques for clear pear juice and its antioxidant activities of 'Chuwhangbae' pear

추황배 청징배즙의 제조 및 항산화 활성

  • Choi, Jin-Ho (Pear Research Station, National Institute of Horticultural and Herbal Science) ;
  • Yim, Sun-Hee (Pear Research Station, National Institute of Horticultural and Herbal Science) ;
  • Choi, Jang-Jeon (Pear Research Station, National Institute of Horticultural and Herbal Science) ;
  • Kim, Sung-Jong (Pear Research Station, National Institute of Horticultural and Herbal Science) ;
  • Nam, Seung-Hee (Food Research Institute, Jeonnam Agricultural Research and Extension Services) ;
  • Kang, Sam Seok (Pear Research Station, National Institute of Horticultural and Herbal Science) ;
  • Kim, Yoon Kyeong (Pear Research Station, National Institute of Horticultural and Herbal Science) ;
  • Lee, Han Chan (Pear Research Station, National Institute of Horticultural and Herbal Science)
  • 최진호 (국립원예특작과학원 배시험장) ;
  • 임순희 (국립원예특작과학원 배시험장) ;
  • 최장전 (국립원예특작과학원 배시험장) ;
  • 김성종 (국립원예특작과학원 배시험장) ;
  • 남승희 (전남농업기술원 식품연구소) ;
  • 강삼석 (국립원예특작과학원 배시험장) ;
  • 김윤경 (국립원예특작과학원 배시험장) ;
  • 이한찬 (국립원예특작과학원 배시험장)
  • Received : 2013.06.12
  • Accepted : 2013.08.26
  • Published : 2013.10.30

Abstract

This study was carried out to optimize the filtration, clarification, anti-browning processing conditions of clear pear juice and to investigate changes in antioxidant activity of pear juice produced through different heating treatment. For the filtration with cheese cloth, filter paper, or centrifugation (10 min at 3,000 rpm), the pear juice was most efficiently filtered with centrifugation because it showed the highest lightness (L value) and lowest yellowness (a value). Among various clarifying agents, 1% of gelatin or bentonite clarified effectively pear juice but tannin or egg albumin did not. Among anti-browning agents (0.1%) like L-ascorbic acid, NaCl or citric acid, L-ascorbic acid prevented the browning of pear juice with the lowest browning index value (2.62), compared to that of NaCl (2.74), or citric acid (2.87). Fructose, sucrose, glucose and sorbitol were present in the pear juice, the fructose and glucose contents increased but that of sucrose decreased in the heated pear juice. The total polyphenol content of the heated pear juice significantly increase, and did the total flavonoid contents in the clear and heated pear juice. The DPPH radical scavenging activity and nitrate scavenging activity were higher in the clear and heated pear juice than in the fruit crush.

본 연구는 배 과즙의 가공조건을 최적화하기 위해 여과, 청징 및 갈변 방지 방법 및 가공 조건에 따른 항산화능을 조사하였다. 배를 분쇄 착즙 후 면포여과, 감압여과 및 원심분리를 실시한 결과 원심분리(3000 rpm, 10분간)를 할 경우 colorimetry value가 높은 'L'값(34.31)과, 낮은 'a'값(-0.13)을 보여 가장 좋은 청징 효과를 나타냈으며, 그 다음으로 감압여과(31.8%) 및 면포여과(11.0%) 순이었다. 청징제 종류에 따른 청징 효과는 1%의 gelatin이나 bentonite가 우수하였으며, tannin과 egg albumin은 효과가 없었다. 갈변저해제 처리에 따른 갈색도 측정결과 0.1% L-ascorbic acid 처리 시 11.5%의 갈변억제 효과를 보였다. 유리당은 fructose, sucrose, glucose, sorbitol이 검출되었으며, 가열배즙에서 fructose와 glucose는 증가되는 반면 sucrose는 감소되었다. 가공 조건에 따른 항산화능 조사 결과 총폴리페놀 함량은 가열배즙이 청징배즙에 비해 유의하게 많아졌으나 총플라보노이드 함량은 청징배즙과 가열배즙이 유사하게 증가하였다. DPPH radical 소거활성과 아질산염 소거능은 가열배즙과 청징배즙에서 착즙액보다 유의적으로 높은 것을 확인할 수 있었다.

Keywords

References

  1. Lee KY, Ko KC, Kim KR, Kim SB, Kim JH, Kim JC, Park DB, Park YB, Byun JK, Chim KK, Won SH, Lee DC, Lee HJ, Jung ST, Han KP, Han HY (1983) Special treatise on fruit crops and horticulture. Hyangmunsa press, Seoul, Korea, p 142-156
  2. Son DS, Kang SJ, Jeong SB, Kim JK, Cho KS, Choi YM, Hong KJ, Choi JJ, Kang SS (2004) Phytochemical and health effect of pear. RDA Semyung Press, Suwon, Korea, p 1-37
  3. RDA (2003) Anniversary of fifty year in horticulture reasearch institute. Scientific Horticulture Press, Seoul, Korea, p 270-360
  4. Seo JH, Hwang YS, Chun JP, Lee CJ (2000) Influence of Pre and Post-harvest treatments on the occurrence of skin browning and fruit quality in 'Niitaka' pears. J Korean Soc Hort Sci, 41, 602-606
  5. An BJ, Lee JT, Gwag JH, Park JM, Lee JY, Son JH, Bae JH Choi C (2004) Biological activity of polyphenol group fraction from Korean pear peel. J Korean Soc App Biol Chem, 47, 92-95
  6. Ruth BA, Sonego L (1979) Changes in pectic substances in ripening pears. J Amer Soc Hort Sci, 104, 500-505
  7. Yoo WJ, Kim DH, Lee DH, Byun JK (2002) Changes in respiration rates, cell wall components and their hydrolase activities during the ripening of 'Whangkeumbae' pear fruit. J Korean Soc Hort Sci, 43, 43-46
  8. Park YS (1999) Carbon dioxide-induced flesh browning development as related to phenolic metabolism in 'Niitaka' pear during storage. J Korean Soc Hort Sci, 40, 567-570
  9. Kim JH, Kim SB, Hong SB, Kim JY, Moon KY, Kim MD, Lee DK (1986) A new late-season pear cultivar, 'Chuwhangbae'. Res Rpt RDA Hort, 28, 57-61
  10. Hwang YS, Chun JP, Lee JC Seo JH (2001) Storage response of 'Kamchun' and 'Chuhwang' pears by harvest dates. J Korean Soc Hort Sci, 19, 48-53.
  11. MIFAFF (2011) Fruit processing information in 2010. MIFAFF 11-1541000-000046-10
  12. Park YM, Kim JK (1997) Characterization of the Degradation of pear fruit cell wall by pectolytic enzymes and their use in fruit tissue liquefaction. J Korean Soc Hort Sci, 38, 255-262
  13. Choi JH, Kim KY, Lee JC (1998) Effects of pre-pressing condition on quality of pear juice. Korean J Food Sci Technol, 30, 827-831.
  14. Hwang IG, Woo KS, Kim TM, Kim DJ, Yang MH, Jeong HS 2006. Change of physiological characteristics of Korean pear (Pyrus pyrifolia Nakai) juice with heat treatment conditions. Korean J food Sci. Technol, 38, 342-347
  15. AOAC. 1990. Official methods of analysis of the AOAC. 15th ed. Association of Official Analytical Chemists, Washington, DC, USA
  16. Wilson AM, Work TM, Bushway AA, Bushway RJ (1981) HPLC determination of fructose, glucose and sucrose in potatoes. J Food Sci, 46, 300-306. https://doi.org/10.1111/j.1365-2621.1981.tb14589.x
  17. Velioglu YS, Mazza G, Cao L, Oomah BD (1998) Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J Agric Food Chem, 46, 4113-4117 https://doi.org/10.1021/jf9801973
  18. NFRI (1990) Manuals of quality characteristic analysis for food quality evaluation II. National Food Research Institute, Skuba
  19. Ohmori Y, Ito M, Kishi M, Mizutani H, Katada T, Konishi H (1995) Antiallergic constituents from oolong tea stem. Biol Pharm Bull, 18, 683-686 https://doi.org/10.1248/bpb.18.683
  20. Gray JI. Dugan JLR (1975) Inhibition of N-nitrosoamine formation in model food system, J Food Sci, 40, 978-985.
  21. Kim IS, Lee JY, Rhee SJ, Youn KS, Choi SW (2004) Preparation of minimally processed mulberry (Morus spp.) juices. Korean J Food Sci Technol, 36, 321-328
  22. Shon KS, Seog EJ, Lee JH (2006) Quality characteristics of clarified apple juice produced by various methods. Korean J Food Preserv, 13, 138-143
  23. Shon KS, Seog EJ, Lee JH (2006) Quality changes of orange juice as influenced by clarification methods. J Korean Soc Food Sci Nurt, 35, 378-382 https://doi.org/10.3746/jkfn.2006.35.3.378
  24. Kim DH (1999) Studies on the production of vinegar from fig. J Kor Soc Food Sci Nutr, 28, 53-60
  25. Kim DH, Rhim JW, Jung ST (2010) Clarification and aging of fermented honey wine. Korean J Food Sci Technol, 31, 1130-1136
  26. Leea WC, Yusofa S, Hamidb NSA, Baharina BS (2007) Effects of fining treatment and storage temperature on the quality of clarified banana juice. Swiss Soc Food Sci Technol, 40, 1755-1764
  27. Choi OJ, Park HR, Chough SH (1998). variation of free sugar and amino acid contents of pear during the ripening period. Korean J Soc Food Sci, 14, 250-254
  28. Hwang CR, Oh SH, Kim HY, Lee SH, Hwang IG, Shin YS, Lee JS, Jeong HS (2011) Chemical composition and antioxidant activity of Deoduk (Codomopsis lanceilata) and Doragi (Platycodon grandiflorum) according to temperature. J Korean Soc Food Sci Nutr, 40, 798-803 https://doi.org/10.3746/jkfn.2011.40.6.798
  29. Kim JH, Joo HK (1989) Change in sugar composition of ginseng extract during heat treatment. Korean J Ginseng Sci, 13, 56-59
  30. Cho HY, Hong SI, Kim YS, Pyun YR. (1993) Prediction of sucrose hydrolysis rate using equivalent time at a reference temperature under regular temperature fluctuations. Korean J Food Sci Technol, 25, 643-648
  31. Dewanto V, Wu X, Adom KK, Liu RH (2002) Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. J Agric Food Chem 50, 3010-3014. https://doi.org/10.1021/jf0115589
  32. Kim GH, Cho SD, Kim DM (1999) Quality evaluation of minimally processed Asian pears. Korean J Food Sci Technol, 31, 1523-1528
  33. Choi Y, Lee SM, Chun J, Lee HB, Lee J (2006) Influence of heat treatment on the antioxidant activities and polyphenolic compounds of Shiitake (Lentinus edodes) mushroom. Food Chem, 99, 381-387 https://doi.org/10.1016/j.foodchem.2005.08.004
  34. Jeong SM, Kim SY, Kim DR, Jo SC, Nam KC, Ahn DU, Lee SC (2004) Effect of heat treatment on the antioxidant activity of extracts from citrus peels. J Agric Food Chem, 52, 3389-3393 https://doi.org/10.1021/jf049899k
  35. Lee JS, Jang HH, Song GY (2013) Changes in quality characteristics and bioactivity of Rehmanniae Radix jung kwa obtained by different heat processing times. Korean J Aesthet Cosmetol, 11, 59-69
  36. Shin JH, Jung KM, See SJ, Yang SM, Rue GH, Sung NJ (2009) Biological activities of dried garlic, red ginseng and their mixture. J Korean Soc Food Sci Nutr, 38, 1633-1639 https://doi.org/10.3746/jkfn.2009.38.12.1633
  37. Lee YR, Woo KS, Hwang IG, Kim HY, Lee SH, Lee JS, Jeong HS (2012) Physicochemical properties and antioxidant activities of garlic (Allium sativum L.) with different heat and pressure treatments. J Korean Soc Food Sci Nutr, 41, 278-282 https://doi.org/10.3746/jkfn.2012.41.2.278
  38. Lee KS, Kim Gh, Kim HH, Kim ES, Park HM, Oh MJ (2009) Physiological functionalities of tea thermally processed from Ixeris dentata root. J Korean Soc Food Sci Nutr, 38, 496-501 https://doi.org/10.3746/jkfn.2009.38.4.496

Cited by

  1. Fruit quality and core breakdown of ‘Wonhwang’ pears in relation to harvest date and pre-storage cooling vol.188, 2015, https://doi.org/10.1016/j.scienta.2015.03.011
  2. Enzymatic browning inhibition and antioxidant activity of pear juice from a new cultivar of asian pear (Pyrus pyrifolia Nakai cv. Sinhwa) with different concentrations of ascorbic acid vol.25, pp.1, 2016, https://doi.org/10.1007/s10068-016-0023-9
  3. 녹차와 로즈마리 추출물을 첨가한 두유의 항산화 활성 vol.24, pp.6, 2013, https://doi.org/10.11002/kjfp.2017.24.6.871
  4. Quality Characteristics of Breads Added with Pear Juice Prepared by Different Heat Treatments vol.29, pp.5, 2013, https://doi.org/10.17495/easdl.2019.10.29.5.392