Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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In vitro screening of the acetylcholinesterase inhibition, antioxidant activity, and neuronal cell protective effect of medicinal plant extracts

생약추출물의 acetylcholinesterase 저해, 항산화 및 신경세포보호 효과 in vitro 탐색

  • Um, Min Young (Division of Metabolism and Functionality Research, Korea Food Research Institute) ;
  • Ha, Tae Youl (Division of Metabolism and Functionality Research, Korea Food Research Institute) ;
  • Seong, Ki Seung (Division of Metabolism and Functionality Research, Korea Food Research Institute) ;
  • Kim, Yong Sik (Department of Hotel Culinary Arts, Yeonsung University)
  • 엄민영 (한국식품연구원 대사영양연구단) ;
  • 하태열 (한국식품연구원 대사영양연구단) ;
  • 성기승 (한국식품연구원 대사영양연구단) ;
  • 김용식 (연성대학교 호텔조리과)
  • Received : 2013.08.28
  • Accepted : 2013.10.29
  • Published : 2013.12.30
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Abstract

This study investigated the effects (i.e., the acetylcholinesterase activity, lipid peroxidation, and neuronal survival) of 20 kinds of medicinal water extracts. The water extracts of three medicinal plants - Cornus officinalis, Glycyrrhiza glabra, and Angelica gigas - were found to be the most effective on acetylcholinesterase inhibitory activity. In the lipid peroxidation-generating system induced by $H_2O_2/FeSO_4$ in rat brain homogenates, Perilla frutescens, Polygonum multiflorum, Cinnamomun cassia, and G. glabra exhibited protective activity against lipid peroxidation. The neuronal cell death induced by L-glutamate in PC12 was suppressed by the water extracts of G. glabra, Cinnamomun cassia, Platycodon grandiflorum, and Mentha arvensis at the concentration of $100{\mu}g/mL$. Taken together, these results showed that the water extract of G. glabra has the potential anti-dementia activity, which suggests that it might provide an effective strategy for improving dementia.

본 연구에서는 20종 생약 열수추출물의 AChE 활성, 산화스트레스로 인한 지질 과산화물 생성 억제능 및 뇌신경세포 사멸에 대한 보호효과를 비교하였다. AChE의 억제활성은 산수유, 감초, 당귀 열수추출물에서 우수하였으며, 그 중 산수유가 가장 높은 억제율을 나타내었다. 또한 $H_2O_2/FeSO_4$로 산화 스트레스를 유발시켜 생약 열수추출물의 지질과산화물 생성 억제 활성을 조사한 결과 소엽, 하수오, 계피 및 감초 열수추출물에서 높은 항산화 활성을 보였다. L-Glutamate에 의해 유도된 신경세포 독성에 대한 보호효과는 감초, 계피, 길경, 박하 열수추출물의 $100{\mu}g/mL$ 농도에서 관찰되었다. 본 연구를 종합적으로 살펴보았을 때, 감초 열수추출물이 치매 예방 및 개선제로써의 활용가능성이 가장 뛰어난 것으로 판단된다. 향후 치매예방 효능을 가지는 새로운 화합물 발굴을 위한 기초자료로 활용될 것으로 사료된다.

Keywords

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