Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Comparison of Biofilm Formed on Stainless Steel and Copper Pipe Through the Each Process of Water Treatment Plant

정수처리 공정 단계별 스테인리스관과 동관에 형성된 생물막 비교

  • Kim, Geun-Su (Safety Center, Pulmuone Holdings Co., Ltd.) ;
  • Min, Byung-Dae (Water Supply and Sewerage Research Division, Environmental Infrastructure Research Department, National Institute of Environmental Research, Environmental Research Complex) ;
  • Park, Su-Jeong (Water Supply and Sewerage Research Division, Environmental Infrastructure Research Department, National Institute of Environmental Research, Environmental Research Complex) ;
  • Oh, Jung-Hwan (Environmental Analysis & Research team, The Environment Technology Institute, WoongjinCoway CO., Ltd.) ;
  • Cho, Ik-Hwan (Waterworks Head quarters) ;
  • Jang, Seok-Jea (Water Supply and Sewerage Research Division, Environmental Infrastructure Research Department, National Institute of Environmental Research, Environmental Research Complex) ;
  • Kim, Ji-Hae (Water Supply and Sewerage Research Division, Environmental Infrastructure Research Department, National Institute of Environmental Research, Environmental Research Complex) ;
  • Park, Sang-Min (Water Supply and Sewerage Research Division, Environmental Infrastructure Research Department, National Institute of Environmental Research, Environmental Research Complex) ;
  • Park, Ju-Hyun (Water Supply and Sewerage Research Division, Environmental Infrastructure Research Department, National Institute of Environmental Research, Environmental Research Complex) ;
  • Chung, Hyen-Mi (Water Supply and Sewerage Research Division, Environmental Infrastructure Research Department, National Institute of Environmental Research, Environmental Research Complex) ;
  • Ahn, Tae-Young (Department of Microbiology, Dankook University) ;
  • Jheong, Weonhwa (Water Supply and Sewerage Research Division, Environmental Infrastructure Research Department, National Institute of Environmental Research, Environmental Research Complex)
  • 김근수 (풀무원) ;
  • 민병대 (국립환경과학원 상하수도연구과) ;
  • 박수정 (국립환경과학원 상하수도연구과) ;
  • 오정환 (웅진코웨이) ;
  • 조익환 (인천시 상수도사업본부) ;
  • 장석재 (국립환경과학원 상하수도연구과) ;
  • 김지혜 (국립환경과학원 상하수도연구과) ;
  • 박상민 (국립환경과학원 상하수도연구과) ;
  • 박주현 (국립환경과학원 상하수도연구과) ;
  • 정현미 (국립환경과학원 상하수도연구과) ;
  • 안태영 (단국대학교 미생물학과) ;
  • 정원화 (국립환경과학원 상하수도연구과)
  • Received : 2013.11.01
  • Accepted : 2013.11.29
  • Published : 2013.12.31
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Abstract

Biofilm formed on stainless and copper in water treatment plant was investigated for sixteen weeks. Biofilm reactor was specially designed for this study. It was similar to that of a real distribution pipe. Raw water, coagulated, settled, filtered and treated water were used in this study. The average number of heterotrophic bacteria counts was $1.6{\times}10^4CFU/ml$, $5.8{\times}10^3CFU/ml$, $1.8{\times}10^3CFU/ml$, $1.3{\times}10^2CFU/ml$, 1 CFU/ml, respectively. Density of biofilm bacteria formed on stainless and copper pipes in raw, coagulated and settled water increased above $2.9{\times}10^3CFU/cm^2$ within second weeks while more biofilm bacteria counts were found on the stainless pipe than on the copper pipe. In case of filtered water (free residue chlorine 0.44 mg/L), there was no significant difference in the number of biofilm bacteria on both pipes and biofilm bacteria below $18CFU/cm^2$ were detected on both pipe materials after fifth weeks. Biofilm bacteria were not detected on both pipe materials in treated water (free residue chlorine 0.88 mg/L). According to the results of DGGE analysis, Sphingomonadacae was a dominant species of biofilm bacteria formed on the stainless pipe while the copper pipe had Bradyrhizobiaceae and Sphingomonadaceae as dominant bands. In case of filtered water, a few bands (similar to Propionibacterium sp., Sphingomonas sp., Escherichia sp., and etc.) that have 16S rRNA sequences were detected in biofilm bacteria formed on both pipes after fifth weeks. Stainless pipe had higher species richness and diversity than the copper pipe.

정수처리 시설에서 급 배수관으로 많이 사용되는 스테인리스관과 동관에 형성되는 생물막의 특성에 대해 16주 동안 조사하였다. 생물막 반응기는 실제 배급수관의 구조와 유사하게 설계하였으며, 정수처리장으로 유입되는 상수원수와 약품혼화 응집수, 침전수, 여과수, 처리수를 사용하였다. 평균 종속영양세균수는 $1.6{\times}10^4CFU/ml$, $5.8{\times}10^3CFU/ml$, $1.8{\times}10^3CFU/ml$, $1.3{\times}10^2CFU/ml$, 1 CFU/ml로 각 처리 과정을 거치면서 감소하였다. 스테인리스관과 동관에 형성된 생물막 세균수는 원수, 응집수, 침전수에서 2주만에 $2.9{\times}10^3CFU/cm^2$ 이상으로 증가하였고, 동관보다 스테인리스관에서 생물막 세균수가 높게 검출되었다. 여과수(평균 잔류염소 0.44 mg/L)에서는 두 관 재질에 따른 생물막 세균수의 명확한 차이는 없었으며, 5주 이후부터 두 관재질 모두 $18CFU/cm^2$ 이하의 생물막 세균이 검출되었다. 정수(평균 잔류염소 0.88 mg/L)에서는 두 관 재질 모두 생물막 세균이 검출되지 않았다. DGGE 분석결과, 원수, 응집수, 침전수에서 스테인리스관은 Sphingomonadaceae가 우점이였고, 동관에서는 Bradyrhizobiaceae와 함께 Sphingomonadaceae도 우점이였다. 여과수의경우, 5주차 이후 스테인리스관과 동관에 형성된 생물막에서 Propionibacterium sp., Sphingomonas sp., Escherichia sp. 등과 유사한 16S rRNA 유전자 서열을 가지는 밴드들이 검출되었다. 종 풍부도 및 다양성은 동관에 비해 스테인리스관이 더 높게 나타났다.

Keywords

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