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Characteristics and Virulence Assay of Entomopathogenic Fungus Metarhizium anisopliae for the Microbial Control of Spodoptera exigua

파밤나방의 생물적 방제를 위한 곤충병원성 곰팡이 Metarhizium anisopliae의 특성 및 병원성 검정

  • Han, Ji Hee (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA) ;
  • Kim, Hyeonggyeong (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA) ;
  • Leem, Hun Tae (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA) ;
  • Kim, Jeong Jun (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA) ;
  • Lee, SangYeob (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA)
  • 한지희 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 김형경 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 임훈태 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 김정준 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 이상엽 (농촌진흥청 국립농업과학원 농업미생물과)
  • Received : 2013.11.08
  • Accepted : 2013.12.20
  • Published : 2013.12.31

Abstract

Beet armyworm, Spodoptera exigua is difficult to control using chemical insecticides because of the fast development of insecticide resistance. For eco-friendly beet armyworm managements, various control agents are required. Entomopathogenic fungus is one of the promise control agents as an alternative to chemical control agent. We isolated entomopathogenic fungi from soil samples of Yangpyeong, Gyeonggi-do by insect-bait method using Tenebio molitor and conducted bioassay to larva of beet armyworm. The result of bioassay, a selected strain FT83 showed 100% mortality against third instar larva of S. exigua. On the basis of morphological characteristics and analysis of 18srRNA sequence for ITS, the strain FT83 was identified as a Metarhizium anisopliae. The mortality of beet armyworm showed $81.6{\pm}9.3%$ at $1{\times}10^6$ conidia/ml, 100% at $1{\times}10^7$ conidia/ml and 100% at $1{\times}10^8$ conidia/ml respectively. Therefore, we recommend to proper control efficacy against S. exigua in which more than $1{\times}10^7$ conidia/ml suspension of M. anisopliae FT83.

난방제 해충의 하나인 파밤나방의 생물적 방제를 위하여 곤충병원성 곰팡이를 수집, 분리하여 파밤나방에 고병원성인 곰팡이를 선발하는 연구를 수행하였다. 곤충병원성 곰팡이의 수집은 친환경 농업지역인 경기도 양평 지역의 수집된 토양 샘플에 갈색거저리를 넣어 감염된 곤충에서 곰팡이를 분리, 수집하는 insect-bait법을 활용하였다. 분리한 곤충병원성 곰팡이를 파밤나방 3령 유충에 처리한 결과, FT83균주가 파밤나방에 병원성이 높았으며, 현미경을 이용한 형태학적 분석과 18srRNA의 ITS sequencing을 통한 분자생물학적 방법을 이용하여 동정한 결과 Metarhizium anisopliae로 동정되었다. 선발한 M. anisopliae FT83의 온도별 균사생장속도는 $15^{\circ}C$에서 $30^{\circ}C$까지는 온도가 증가할수록 균사의 생장이 촉진되었으나 35에서는 균사가 생장하지 않았다. 선발 균주의 농도별 살충율은 $1{\times}10^6$ conidia/ml에서 $81.6{\pm}9.3%$, $1{\times}10^7$ conidia/ml에서 100%, $1{\times}10^8$conidia/ml에서 100%로 $1{\times}10^7$ conidia/ml 농도가 경제적인 처리농도였다.

Keywords

References

  1. Charnley, A. K. (1997) Entomopathogenic fungi and their role in pest control, The mycota IV environmental and microbial relationships, Berlin, Springer., 185-201.
  2. Gho, H. G., S. G. Lee, B. P. Lee, K. M. Choi and J. W. Kim (1991) Simple mass-rearing of beet armyworm, Spodoptera exigua (Hubner)(Lepidoptera: Noctuidae), on an aritificial diet. Kor. J. Appl. Entomol. 29:180-183.
  3. Hajek A. E. and R. J. St. Leger (1994) Interactions between fungal pathogens and insect hosts. Annu. Rev. Entomol., 39: 293-322. https://doi.org/10.1146/annurev.en.39.010194.001453
  4. Jin, N. Y., S. Y. Jung, C. Park, S. K. Paek, M. J. Seo and Y. Y. Man (2009) The synergy effects of mixed treatment with tannic acid and Bacillus thuringiensis subsp. kurstaki KB100 against Spodoptera exigua. Kor. J. Appl. Entomol., 48(4):519-526. https://doi.org/10.5656/KSAE.2009.48.4.519
  5. Kang, E. J., M. G. Kang, M. J. Seo, S. N. Park, C. U. Kim and Y. M. Yu (2008) Toxicological effects of some insecticides against welsh onion beet armyworm (Spodoptera exigua). Kor. J. Appl. Entomol. 47:155-162. https://doi.org/10.5656/KSAE.2008.47.2.155
  6. Lacey, L. A., R. Frutos, H. K. Kaya and P. Vail (2001) Insect pathogens as biological control agents : Do they have a future? Biol. Control. 21:230-248. https://doi.org/10.1006/bcon.2001.0938
  7. Lawrence A. Lacey (2012) Manual of techniques in invertebrate pathology. Second edition, 179-180.
  8. Marcos R. de Faria, Stephen P. Wraigh (2007) Mycoinsecticides and mycoacaricides : A comprehensive list with worldwide coverage and international classification of formulation types. Biol. Control. 43:237-256. https://doi.org/10.1016/j.biocontrol.2007.08.001
  9. Meyling, N. V. (2007) Methods for isolation of entomopathogenic fungi from the soil environment. Manual for isolation of soil borne entomopathogenic fungi. p. 2-18.
  10. Moulton, J. K., D. A. Peppe, J. Dennehy, P. Dugger and D. Richter (1999) Studies of resistance of beet armyworm (Spodoptera exigua) to spinosad in field populations from the southern USA and southeast Asia. Proceedings of the Beltwide Cotton Conferences. Orlando. FL. USA 2, 884-887
  11. Muhammad, R. H., B. H. Qiong, Y. H. Mei, Z. Guohua and W. Qunfan (2009) Study of destruxin B and tea saponin, their interaction and synergism activities with Bacillus thuringiensis kurstaki against Spodoptera exigua (Huber) (Lepidoptera: Noctuidae). Appl. Entomol. Zool. 44(3):419-428. https://doi.org/10.1303/aez.2009.419
  12. Shoaib, F., A. S. Mushtaq, M. B. Khan and N. Muhammad (2012) Prevalence and effectiveness of Metarhizium anisopliae against Spodoptera exigua (Lepidoptera: Noctuidae) in Southern Punjab, Pakistan. Pakistan J. Zool., 44(3):753-758.
  13. Supakdamrongkul, P., A. Bhumiratana and C. Wiwat (2010) Characterization of an extracellular lipase from the biocontrol fungus, Nomuraea rileyi MJ and its toxicity toward Spodoptera litura. J. Invertebr. Pathol. 105:229-235.
  14. Tabashnik, B. E. and Y. Carriere (2004) Bt transgenic crops do not have favorable effects on resistant insects. J. Insect Sci. 4:1-4.

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