Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Isoflavones and biotransformed dihydrodaidzein production with in vitro cultured callus of Korean wild arrowroot Pueraria lobata

한국산 야생칡 캘러스에서의 이소플라본 및 생물전환에 의한 디하이드로다이드제인 분석

  • Lee, Eunji (Department of Horticulture and Institute of Life and Environment, Daegu University) ;
  • Kwon, Jung Eun (Department of Life Science, Gachon University) ;
  • Kim, Soojung (Department of Horticulture and Institute of Life and Environment, Daegu University) ;
  • Cha, Min-Seok (Department of Horticulture and Institute of Life and Environment, Daegu University) ;
  • Kim, Inhye (Functional Food & Nutrition Division, National Academy of Agricultural Science, RDA) ;
  • Kang, Se Chan (Department of Life Science, Gachon University) ;
  • Park, Tae-Ho (Department of Horticulture and Institute of Life and Environment, Daegu University)
  • 이은지 (대구대학교 원예학과, 대구대학교 생명환경연구소) ;
  • 권정은 (가천대학교 생명과학과) ;
  • 김수정 (대구대학교 원예학과, 대구대학교 생명환경연구소) ;
  • 차민석 (대구대학교 원예학과, 대구대학교 생명환경연구소) ;
  • 김인혜 (농촌진흥청 국립농업과학원) ;
  • 강세찬 (가천대학교 생명과학과) ;
  • 박태호 (대구대학교 원예학과, 대구대학교 생명환경연구소)
  • Received : 2013.12.16
  • Accepted : 2013.12.23
  • Published : 2013.12.31
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Abstract

Pueraria lobata, a medicinally important leguminous plant produces various isoflavones including puerarin, daidzin and daidzein which are metabolized to equol via dihydrodaidzein and tetrahydrodaidzein by the bacterial fermentation of natural isoflavone sources in human intestines. In this study, we described callus proliferation and isoflavone production in callus of Korean wild arrowroot and dihydrodaidzein biosynthesis in callus extract fermented with Pediococcus pentosaceus. Proliferation was the best at callus cultured in the medium containing 1.0 mg/L TDZ and 1.0 mg/L NAA at light condition for 12 days. Puerarin was significantly more produced at callus cultured in the medium containing 2.0 mg/L kinetin and 1.0 mg/L NAA at dark condition for 16 days, but daidzin and daidzein were not significant. Callus extract was successfully fermented with P. pentosaceus and dihydrodaidzein, which is one of equol precursors formed by biotransformation, was confirmed to be produced. These results will facilitate mass production of callus and isoflavones as equol precursors from Korean wild arrowroot and can be applied for the production of equol by biotransformation in vitro.

국내 자생하는 야생칡(Pueraria lobata)은 푸에라린, 다이드진, 다이드제인 등 다양한 이소플라본을 함유하고 있는 주요한 콩과 약용작물이다. 이들 이소플라본은 장내에서 특정 세균과 반응으로 생물전환이 일어나 디하이드로다이드제인, 테트라하이드로다이드제인을 거쳐 약리작용이 타 이소플라본 보다 월등한 이퀄로 물질대사가 이루어지는 것으로 알려져 있다. 본 연구에서는 국내 야생칡으로부터 유도된 캘러스를 이용하여 배양배지, 배양조건 및 배양기간에 따른 캘러스의 증식과 이소플라본의 합성 정도를 구명하고 배양된 캘러스 추출물을 생물전환 능력이 있는 것으로 밝혀진 Pediococcus pentosaceus 균주와의 반응을 통하여 이퀄 전구체인 디하이드로다이드제인의 생합성을 구명하고자 수행되었다. 캘러스의 증식은 광조건에서 1.0 mg/L TDZ과 1.0 mg/L NAA가 첨가된 배지에서 12일 배양했을 때 가장 좋다. 푸에라린 함량은 암조건에서 2.0 mg/L kinetin과 1.0 mg/L NAA가 첨가된 배지에서 16일 배양했을 때 가장 높게 나타났으나, 다이드진과 다이드제인 함량은 처리에 따른 유의한 차이가 나타나지 않았다. 캘러스 추출물을 P. pentosaceus 균주와 반응하여 발효시켰을 때 생물전환에 의해 이퀄의 전구체인 디하이드로다이드제인이 성공적으로 생합성되는 것이 확인되었다. 본 연구의 결과는 국내 야생칡을 대상으로 캘러스의 대량증식을 통한 이퀄의 전구물질인 이소플라본의 대량생산에 기여할 수 있으며, 기내에서 생물전환을 통하여 이퀄을 생산하기 위한 기반이 될 것이다.

Keywords

References

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