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Antioxidant Activities in Freeze-dried and Hot Air-dried Schizandra Fruit (Schizandra chinensis Baillon) at Different Microwave-asssisted Extraction Conditions

마이크로웨이브 추출조건에 따른 동결 및 열풍 건조 오미자 추출물의 항산화 특성

  • Park, Eun-Joo (School of Food Science & Biotechnology, Kyungpook National University) ;
  • Ahn, Jae-Jun (School of Food Science & Biotechnology, Kyungpook National University) ;
  • Kim, Jeong-Sook (Department of Food Nutrition & Culinary, Keimyung College) ;
  • Kwon, Joong-Ho (School of Food Science & Biotechnology, Kyungpook National University)
  • 박은주 (경북대학교 식품공학부) ;
  • 안재준 (경북대학교 식품공학부) ;
  • 김정숙 (계명문화대학 식품영양조리과) ;
  • 권중호 (경북대학교 식품공학부)
  • Received : 2013.05.09
  • Accepted : 2013.06.25
  • Published : 2013.12.31

Abstract

This study was aimed to investigate the effect of microwave-assisted extraction on Schizandra fruit extract dried by two different treatments: freeze-drying and hot air-drying. Two extraction parameters were measured in particular: total polyphenol content and antioxidant activity. Both values were found to increase as the microwave power increased, for both drying processes. However, the extract from the freeze-dried sample exhibited higher antioxidant activity than that from the hot air-dried samples. Additionally, the total polyphenol content and antioxidant activity of the extract from the freeze-dried samples increased with the extraction time, whereas they decreased with the extraction time in the case of the hot air-dried sample. Solvent concentration was also found to have a significant effect on total polyphenol content and antioxidant activity; the highest values for both properties were achieved at 50 and 70% ethanol concentrations, respectively. In summary, a higher total polyphenol content and antioxidant activity were observed for Schizandra fruit extracted by freeze-drying than that by hot air-drying.

본 연구는 열풍 건조와 동결 건조한 오미자를 시료로 하여 마이크로웨이브 추출 조건(W, 시간, 에탄올 농도)에 따른 추출물의 항산화 특성을 비교하였다. 추출물의 총 폴리페놀 함량과 항산화능(DPPH, ABTS radical 소거능)은 마이크로웨이브 용량(100-200W)이 증가할수록 동결 및 열풍 건조 모두에서 증가하였다. 추출시간(1-8분)에 따른 총 폴리페놀 함량과 항산화능은 동결건조 시료에서는 증가하였지만, 열풍건조 시료의 경우는 4분까지 증가하다가 이후에는 감소하였다. 용매를 달리한 추출물의 총 폴리페놀 함량과 항산화능은 에탄올 및 물 혼합 용매 추출물(50, 70%)에서 가장 높게 나타났으며, 열수 추출물과 99% 에탄올 추출물은 낮게 나타났다. 건조방법에 따른 총 폴리페놀 함량과 항산화능을 비교하였을 때, 모든 추출 조건에서 동결건조 오미자는 열풍건조 오미자보다 높은 항산화 활성을 나타내었다. 각 추출조건별 실험인자들의 상관성을 분석한 결과, 동결 오미자는 총 폴리페놀 함량과 항산화 활성 간에 높은 상관성을 나타낸 반면, 열풍건조 오미자는 상관성이 상대적으로 낮았다. 이상의 결과에서 건조방법은 오미자 추출물의 항산화적 특성에 유의적인 영향을 미쳤으며, 동결건조 오미자는 열풍건조 오미자보다 높은 총 폴리페놀 함량과 항산화 활성을 나타내었다.

Keywords

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