The inhibitive effect of erythritol on growth and acidogenic ability of Streptococcus mutans

에리스리톨의 Streptococcus mutans에 대한 성장력과 산생성능의 억제효과

  • 박영남 (김천대학교 치위생학과)
  • Received : 2013.10.03
  • Accepted : 2013.12.20
  • Published : 2013.12.28


The purpose of this study was to closely examine the inhibitive effect of erythritol on growth and acidogenic ability of Streptococcus mutans. As expected, the growth of S. mutans was comparably increased with the addition of sucrose. However, xylitol and erythritol remarkably reduced the growth of S. mutans. Growth inhibition was detected at more than 5% of erythritol although xylitol showed growth inhibition effect at all concentrations tested. Growth inhibition effect was monitored with the combination of same concentration of erythritol and other carbohydrates. Combination of 5% or 10% erythritol with xylitol showed effective growth inhibition. Addition of 2.5%, 5%, or 10% erythritol with sorbitol also showed growth inhibition. From these results, erythritol showed potency of growth inhibition of S. mutans, which is involved in dental caries, and was confirmed to be an excellent sugar substitute, which has effect on preventing caries.

최근 대체 감미료로서 당알코올과 합성감미제에 관한 연구가 활발히 진행되고 있다. 에리스리톨은 포도당을 원료로 효모에 의해 생산되는 포도당 발효감미료로서 과실류, 버섯, 포도주, 청주, 간장등의 발효식품에 함유되어 있는 천연당질로 $C_4H_{10}O_4$의 분자구조를 가지는 4탄당의 당알코올이다. 구강내에서 산생성균인 Streptococcus mutans의 증식과 산생성, 치면세균막 형성 및 치아우식증 등은 여러 종류의 영양물질이 혼합되어 있는 구강내에서 일어나는 과정이므로 본 연구는 에리스리톨과 다른 감미제에서 S. mutans의 성장력과 산생성력을 분석하여 에리스리톨이 치아우식증의 예방에 관여하는 중요한 기전을 밝혀내어 치아우식예방을 위한 제품을 생산하거나 활용시 올바른 정보를 제공하고 개선방안을 마련하기 위한 기초자료를 제공하고자 시행하였다. 연구결과 에리스리톨은 우식활성균의 성장을 억제시키고 산생성능을 감소시켜 치아우식을 유발하지 않으며 우식예방효과가 있는 자당 대체 감미제로 우수한 것으로 확인이 되었다.


  1. Kauko K, Makinen KK. Sugar Alcohols, Caries Incidence, and Remineralization of Caries Lesions: A Literature Review. Int J Dent, 981072, 2010
  2. Makinen KK, Isotupa KP, Kivilompolo T, Makinen P-L, Murtomaa S, Petaja J, Toivanen J, Soderling E. The effect of poly-combinant saliva stimulants on S. mutans levels in plaque and saliva of patients with mental retardation. Spec Care Dent, Vol. 22, pp. 187-193, 2002
  3. Makinen KK, Isotupa KP, Kivilompolo T, Makinen P-L, Toivanen J, Soderling E. Comparision of erythritol and xylitol saliva stimulants in the control of dental plaque and mutans streptococci. Caries Res, Vol. 35, pp. 129-135, 2005
  4. Makinen KK, Soderling E, Hurttia H, Lehtonen OP, Luukkala E. Biochemical, microbiological, and clinical comparisions between two dentifrices that contain different mixtures of sugar alcohols. J Am Dent Assoc, Vol. 111, No. 7, pp. 45-751, 1985
  5. Clark JBK, Graham EF, Lewis BA, Smith F. D-Mannitol, sorbitol and glycerol in bovine serum. J Reprod Fertil, Vol. 13, pp. 189-197, 1967
  6. Cock P, Bechert C-L. Erythritol : Functionality in noncaloric functional beverages. Pure Appl chem, Vol. 74, pp. 1281-1289, 2002
  7. Kawanabe J, Hirasawa M, Takeuchi T, Oda T, Ikeda T. Noncariogenicity of erythritol as a substrate. Caries Res, Vol. 26, pp. 358-362. 1992
  8. Y.H .No, K.U. Lee, K.Y. Jang. Inhibittory effects of erythritol on the growth and adsorption to saliva -coated HA beads of some oral bacteria. Korean academy of oral health, Vol. 24, pp. 69-83, 2000
  9. Dodds MJ, Hiseh SC, Johnson DA. The effect of increased mastication by d aily gum-chewing on salivary gland output and dental acidogenicity. J Dent Res, Vol. 70, pp. 1474-1478, 1991
  10. Hanada N. Current understanding of the cause of dental caries. Jpn J Infect Dis, Vol. 53, pp. 1-5, 2000
  11. Gibbons RJ, Fitzgerald RJ. Dectran-induced agglutination of streptococcus mutans and its potential role in the formation of microbial dental plaques. J Bacteriol, Vol. 98, pp. 341-346, 1969
  12. Rolla G, Scheie AA, Ciardi JE. Role of sucrose in plaque formation. Scand J Dent Res, Vol. 93, pp. 105-111, 1985
  13. Dibdin GH, Shellis RP. Physical and biochemical studies of streptococcus mutans sediments suggest new factors linking the cariogenicity of plaque with it's extracellular polysaccharide content. J Dent Res, Vol. 67, pp. 890-895, 1988
  14. Vacca-Smith AM, Bowen WH. Binding properties of streptococcal glucosyltransferase for hydroxyl apatite, saliva-coated hydroxylapatite and bacteri al surfaces. Arch Oral Biol, Vol. 43, pp. 103-110, 1988
  15. Ooshima T, Matsumura M, Hoshino T, Kawabata S, Sobue S, Fujiwara T. Conrtibutions of three glycosyltransferase to sucrose-dependent adherence of streptococcus mutans. J Dent Res, Vol. 80, pp. 1672-1677, 2001
  16. Fujiwara T, Sasada E, Mima N, Ooshima T. Caries prevalence and salivary mutans streptococci in 0-2-year-old children of Japen. Community Dent Oral Epidemiol, Vol. 19, pp. 151-154, 1991
  17. Kuramitsu HK. Virulence factors of mutans streptococci: role of molecular genetics. Crit Rec Oral Biol and Med, Vol. 4, pp. 159-176, 1993
  18. Makinen KK, Alanen P, Isokangas P, Isotupa K, Soderling E, Makinen PL. Thirty-nine-month xylitol chewing-gum program in initially 8-year-old school children : a feasibility study focusing on mutans streptococci and lactobacilli. Int Dent J, Vol. 58, pp. 41-50, 2008
  19. Thaweboon S, Thaweboon B, Soo-Ampon S. The effect of xylitol chewing gum on mutans streptococci in saliva and dental plaque. Southeast Asian J Trop Med Public Health, Vol. 35, pp. 1024-1027, 2004
  20. Soderling E, Makinen KK, Chen CY, Pape HR Jr, Loesche W, Makinen PL. Effect of sorbitol, xylitol and xylitol/sorbitol chewing gums on dental plaque. Caries Res, Vol. 23, pp. 378-384, 1989
  21. Haresaku S, Hanioka T, Tsutsui A, Yamamoto M, Chou T, Gunjishima Y. Long-term effect of xylitol gum use on mutans streptococci in adult. Caries Res, Vol. 41, pp. 198-203, 2007
  22. Birkhed D, Bar A. Sorbitol and dental caries. World Rev Nutr Diet, Vol. 65, pp. 1-37, 1991
  23. K.H Kim, B.C. Jeong, J.S. Oh, G.H. Yang. The effect of xylitol and carbohydrates on streptococcus. Korean academy of pediatric dentistry, Vol. 29, pp. 561-567, 2002
  24. K.H. Shin, G.H. Yang, N.G. Choi, S.M. Kim, J.S. Oh. The effect of xylitol on the lactose fermentation of streptococcus. Korean academy of pediatric dentistry, Vol. 31, pp. 202-211, 2004
  25. Eva S, Aija-Maaria H-L. Xylitol and erythritol decrease adherence of polysaccharide-producing oral Streptococci. Curr Microbiol, Vol. 60, pp. 25-29, 2010
  26. Zucca M, Cenna S, Berzioli S, Gariglio M, Fagnoni V. Streptococcus mutans and dental caries : micro biological aspects. G Bacteriol Virol Immunol, Vol. 83, pp. 108-117, 1990
  27. Jean Claude MP. Anti-cariogenic activity of erythritol. US Patent, 2001