The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Effects of Bletillae Rhizoma on the Elastase, Collagenase, and Tyrosinase Activities and the Procollagen Synthesis in Hs68 Human Fibroblasts

  • Received : 2012.12.27
  • Accepted : 2013.01.15
  • Published : 2013.01.30
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Abstract

Objectives： Bletillae Rhizoma, the roots of Bletilla striata, is used to restrain the leakage of blood and stop bleeding. It can cure the sores, ulcers, and chapped skin. This study was designed to investigate the collagen metabolism, elastase and tyrosinase activity of Bletillae Rhizoma extract (BR). Methods : The effects of BR on type I procollagen production and collagenase activity in human normal fibroblasts Hs68 after UVB (312 nm) irradiation were measured by ELISA method. The elastase activity, tyrosinase activity, and L-DOPA oxidation after treatment of BR were measured as well. Results : In the present study, the collagen production (type I procollagen) was significantly increased to $15.7{\pm}1.8$ ng/ml at a concentration of BR 100 ${\mu}g/ml$ in UVB damaged Hs68 cells. The increased collagenase activity after UVB damage was significantly recovered to $42.7{\pm}0.7%$, $54.5{\pm}3.5%$, and $38.4{\pm}0.9%$ by BR 10, 30, and 100 ${\mu}g/ml$. The activities of BR 10 mg/ml on tyrosinase activity was significantly reduced to $45.1{\pm}8.4%$ as well. However, there were no significant effects on the elastase activity and the L-DOPA oxidation. Conclusion : BR showed the promoting effects of collagen synthesis and inhibitory effects of collagenase activity in Hs68, human normal fibroblast cells. And these could be thought to have the anti-wrinkle effects and whitening effects in vitro. These results suggest that BR may have potential as an anti-aging ingredient in cosmetic treatment.

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References

  1. Bensky D, Clavey S, Stoger E. Materia Medica 3rd Ed. WA : Eastland Press. 2004 : 585-8.
  2. Kim IR, Kim HC, Kuk YB, Park SJ, Park YK, Park JH, Seo BI, Seo YB, Song HJ, Shin MK, Lee YJ, Lee YC, Lee JH, Leem KH, Cho SI, Chung JK, Joo YS, Choi HY. Boncho‐Hak. Seoul : Young‐Lim Press. 2007 : 428-9.
  3. Bieth JG. The elastases. J Soc Biol. 2001 ; 195(2) : 173–9. https://doi.org/10.1051/jbio/2001195020173
  4. Emilia M, Luca S, Francesca B, Luca B, Paolo S, Giuseppe F, Gianbattista B, Carmela M, Luigi M, Mauro L. Topical hemostatic agents in surgical practice. Transfus Apher Sci. 2011 ; 45(3) : 305-11. https://doi.org/10.1016/j.transci.2011.10.013
  5. Tabry H, Farrands PA. Update on anal fistulae: surgical perspectives for the gastroenterologist. Can J Gastroenterol. 2011 ; 25(12) : 675‐80. https://doi.org/10.1155/2011/931316
  6. Kraunsoe JA, Claridge TD, Lowe G. Inhibition of human leukocyte and porcine pancreatic elastase by homologues of bovine pancreatic trypsin inhibitor. Biochemistry. 1996 ; 35(28) : 9090–6. https://doi.org/10.1021/bi953013b
  7. Kim YH, Chung CB, Kim JG, Ko KI, Park SH, Kim JH, Eom SY, Kim YS, Hwang YI, Kim KH. Anti‐wrinkle activity of ziyuglycoside I isolated from a Sanguisorba officinalis root extract and its application as a cosmeceutical ingredient. Biosci Biotechnol Biochem. 2008 ; 72(2) : 303‐11. https://doi.org/10.1271/bbb.70268
  8. Vanni A, Gastaldi D, Giunata G. Kinetic investigations on the double enzyme activity of the tyrosinase mushroom. Ann Chim. 1990 ; 80 : 35-60.
  9. Joshi PC, Carraro C, Pathak MA. Involvement of reactive oxygen species in the oxidation of tyrosine and dopa to melanin and in skin tanning. Biochem Biophys Res Commun. 1987 ; 142(1) : 265‐74. https://doi.org/10.1016/0006-291X(87)90480-3
  10. Yang ZY, Guon GX, Lin ZF. Elastolytic activity from Flavobacterium odoratum. Microbial screening and cultivation, enzyme production and purification. Process Biochem. 1994 ; 29(6) : 427-36. https://doi.org/10.1016/0032-9592(94)85010-0
  11. Muller WE. The origin of metazoan complexity: porifera as integrated animals. Integr Comp Biol. 2003 ; 43(1) : 3‐10. https://doi.org/10.1093/icb/43.1.3
  12. Di Lullo GA, Sweeney SM, Korkko J, Ala‐Kokko L, San Antonio JD. Mapping the ligand‐binding sites and disease‐associated mutations on the most abundant protein in the human, type I collagen. J Biol Chem. 2002 ; 277(6) : 4223‐31. https://doi.org/10.1074/jbc.M110709200
  13. Fisher GJ, Datta SC, Talwar HS, Wang ZQ, Varani J, Kang S, Voorhees JJ. Molecular basis of sun‐induced premature skin ageing and retinoid antagonism. Nature. 1996 ; 379(6563) : 335‐9. https://doi.org/10.1038/379335a0
  14. Shin JY, Hur W, Wang JS, Jang JW, Kim CW, Bae SH, Jang SK, Yang SH, Sung YC, Kwon OJ, Yoon SK. HCV core protein promotes liver fibrogenesis via up‐regulation of CTGF with TGFbeta1. Exp Mol Med. 2005 ; 37(2) : 138‐45. https://doi.org/10.1038/emm.2005.19
  15. Wen KC, Shih IC, Hu JC, Liao ST, Su TW, Chiang HM. Inhibitory Effects of Terminalia catappa on UVB‐Induced Photodamage in Fibroblast Cell Line. Evid Based Complement Alternat Med. 2011 ; 2011 : 904532.