The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Anti-obesity effects of herbal extract YY312 in C57BL/6 mice fed a high-fat diet and 3T3-L1 cells

백모근(白茅根) 청피(靑皮) 오수유(吳茱萸) 복합방(複合方)(YY312)의 고지방식이로 유도된 마우스와 3T3-L1 세포에서 항비만 효과

  • Kang, Insug (Department of Biochemistry and Molecular Biology, School of Medicine, Kyung Hee University) ;
  • Hwang, Keun-Young (Department of Biochemistry and Molecular Biology, School of Medicine, Kyung Hee University) ;
  • Choi, A-Young (Department of Biochemistry and Molecular Biology, School of Medicine, Kyung Hee University) ;
  • Roh, Kughwan (Department of Biochemistry and Molecular Biology, School of Medicine, Kyung Hee University) ;
  • Choi, Ji Hyun (Department of Biochemistry and Molecular Biology, School of Medicine, Kyung Hee University) ;
  • Sim, Yeomoon (Department of Oriental Pharmaceutical Science, College of Pharmacy, Kyung Hee University) ;
  • Park, Yoo Kyoung (Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Oh, Myung Sook (Department of Oriental Pharmaceutical Science, College of Pharmacy, Kyung Hee University)
  • 강인숙 (경희대학교 의과대학 생화학분자생물학교실) ;
  • 황근영 (경희대학교 의과대학 생화학분자생물학교실) ;
  • 최아영 (경희대학교 의과대학 생화학분자생물학교실) ;
  • 노국환 (경희대학교 의과대학 생화학분자생물학교실) ;
  • 최지현 (경희대학교 의과대학 생화학분자생물학교실) ;
  • 심여문 (경희대학교 약학대학 한약학과) ;
  • 박유경 (경희대학교 동서의학대학원 의학영양학과) ;
  • 오명숙 (경희대학교 약학대학 한약학과)
  • Received : 2012.12.09
  • Accepted : 2013.01.12
  • Published : 2013.01.30
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Abstract

Objectives : The purpose of this study was to determine the anti-obesity effect and molecular mechanism of YY312, a herbal extract composed of Imperatae Rhizoma, Citri Unshius Pericarpium Immaturus, and Evodiae Fructus, on a high-fat diet-induced animal model and on 3T3-L1 cells. Methods : C57BL/6 mice were fed for 6 weeks with a normal diet or a high-fat diet (HFD). Then they orally administered daily with 300 mg/kg YY312 for next 10 weeks. Body weight and food consumption were recorded weekly and daily, respectively. Tissue weights, serum lipid, and glucose levels were analyzed at the end of the study. Additionally, the effects of YY312 on adipocyte differentiation in 3T3-L1 cells were examined. After differentiating 3T3-L1 cells were treated with YY312, Oil-red O staining, RT-PCR, and Western blotting were performed for lipid accumulation, mRNA expression of adipogenesis gene, and AMP-activated protein kinase (AMPK) phosphorylation, respectively. Results : YY312-administered mice showed a significant reduction of body weights and abdominal adipose tissue weights. YY312 also reduced the serum levels of triglycerides and total cholesterol, compared with the HFD group. Treatment with YY312 inhibited lipid accumulation and blocked expression of adipogenic transcription factors and lipogenesis genes, including peroxisome proliferator-activated receptor ${\gamma}$, CCAT/enhancer binding protein ${\alpha}$ and fatty acid synthase. YY312 increased AMPK phosphorylation in 3T3-L1 adipocytes. Conclusions : This study showed that herbal extract YY312 has an anti-obesity effect in vitro and in vivo. Thus, YY312 could be developed as a supplement for reduction of body weight gain induced by an HFD.

Keywords

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