The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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The Study of Anti-inflammatory Effect of Hwanggeumjakyak-tang Extract in RAW 264.7 Macrophage

황금작약탕(黃芩芍藥湯)의 RAW 264.7 대식 세포에서의 항염증 효과에 관한 연구

  • Kim, Ma-Ryong (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, and Institute of Biotechnology, Wonkwang University) ;
  • Kang, Ok-Hwa (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, and Institute of Biotechnology, Wonkwang University) ;
  • Kim, Sung-Bae (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, and Institute of Biotechnology, Wonkwang University) ;
  • Kang, Hee-Jung (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, and Institute of Biotechnology, Wonkwang University) ;
  • Kim, Ji-Eun (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, and Institute of Biotechnology, Wonkwang University) ;
  • Hwang, Hyeong-Chil (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, and Institute of Biotechnology, Wonkwang University) ;
  • Kim, In-Won (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, and Institute of Biotechnology, Wonkwang University) ;
  • Kwon, Dong-Yeul (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, and Institute of Biotechnology, Wonkwang University)
  • 김마룡 (원광대학교 약학대학 한약학과, 원광한약연구소, 원광생명공학연구소) ;
  • 강옥화 (원광대학교 약학대학 한약학과, 원광한약연구소, 원광생명공학연구소) ;
  • 김성배 (원광대학교 약학대학 한약학과, 원광한약연구소, 원광생명공학연구소) ;
  • 강희정 (원광대학교 약학대학 한약학과, 원광한약연구소, 원광생명공학연구소) ;
  • 김지은 (원광대학교 약학대학 한약학과, 원광한약연구소, 원광생명공학연구소) ;
  • 황형칠 (원광대학교 약학대학 한약학과, 원광한약연구소, 원광생명공학연구소) ;
  • 김인원 (원광대학교 약학대학 한약학과, 원광한약연구소, 원광생명공학연구소) ;
  • 권동렬 (원광대학교 약학대학 한약학과, 원광한약연구소, 원광생명공학연구소)
  • Received : 2012.12.26
  • Accepted : 2013.01.13
  • Published : 2013.01.30
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Abstract

Objectives : Hwanggeumjakyak-tang (huangqin shaoyao tang, HJT) has been used to treat acute enteritis in traditional oriental medicine. However, there has been a lack of studies regarding the effects of HJT on the inflammatory activities and effector inflammatory disease mechanism about macrophage before is not known. So we examined the effect of HJT water extract on pro-inflammatory mediators in lipopolysaccharide (LPS) - stimulated mouse macrophage, RAW 264.7 cells. Methods : Cells were treated with 2 ug/mL of LPS 1 h prior to the addition of HJT. Cell viability was measured by MTS assay. The production of nitric oxide (NO) was determined by reacting cultured medium with Griess reagent. The expression of cyclooxygenase-2 (COX-2), inducible NO synthase (iNOS) and mitogen-activated protein kinases (MAPKs) was investigated by Western blot, RT-PCR. The content of level of cytokines (prostaglandin (PG) $E_2$, interleukin (IL)-6, IL-12, Tumor necrosis factor-alpha (TNF-${\alpha}$) and monocyte chemoattractant protein-1 (MCP-1)) in media from LPS-stimulated Raw 264.7 cells was analyed by ELISA kit. Results : HJT inhibited the production of NO, $PGE_2$, IL-6 as well as the expressions of iNOS, COX-2 but did not inhibit the production of IL-12, TNF-${\alpha}$, MCP-1 in the murine macrophage, RAW 264.7 cells. HJT also had suppression effects of LPS-induced MAPKs activation Conclusion : These results suggest that HJT has an anti-inflammatory therapeutic potential, which may result from inhibition of MAPK phosphorylation, thereby decreasing the expression of pro-inflammatory genes.

Keywords

References

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