The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
권호 표지
DOI QR코드

DOI QR Code

Antibacterial Effect of Sinhyowoldosan Against Methicillin-Resistant Staphylococcus aureus

신효월도산(神效越桃散)이 메티실린에 내성이 있는 Staphylococcus aureus에 대한 항균활성에 관한 연구

  • Shin, In-Sik (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, and Institute of Biotechnology, Wonkwang University) ;
  • Kang, Ok-Hwa (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, and Institute of Biotechnology, Wonkwang University) ;
  • Joung, Dae-Ki (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, and Institute of Biotechnology, Wonkwang University) ;
  • Kang, Hee-Jung (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, and Institute of Biotechnology, Wonkwang University) ;
  • Kim, Ji-Eun (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, and Institute of Biotechnology, Wonkwang University) ;
  • Hwang, Hyeong-Chil (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, and Institute of Biotechnology, Wonkwang University) ;
  • Kim, In-Won (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, and Institute of Biotechnology, Wonkwang University) ;
  • Kwon, Dong-Yeul (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, and Institute of Biotechnology, Wonkwang University)
  • 신인식 (원광대학교 약학대학 한약학과, 원광한약연구소, 원광생명공학연구소) ;
  • 강옥화 (원광대학교 약학대학 한약학과, 원광한약연구소, 원광생명공학연구소) ;
  • 정대기 (원광대학교 약학대학 한약학과, 원광한약연구소, 원광생명공학연구소) ;
  • 강희정 (원광대학교 약학대학 한약학과, 원광한약연구소, 원광생명공학연구소) ;
  • 김지은 (원광대학교 약학대학 한약학과, 원광한약연구소, 원광생명공학연구소) ;
  • 황형칠 (원광대학교 약학대학 한약학과, 원광한약연구소, 원광생명공학연구소) ;
  • 김인원 (원광대학교 약학대학 한약학과, 원광한약연구소, 원광생명공학연구소) ;
  • 권동렬 (원광대학교 약학대학 한약학과, 원광한약연구소, 원광생명공학연구소)
  • Received : 2012.12.11
  • Accepted : 2013.01.12
  • Published : 2013.01.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : Methicillin-Resistant Staphylococcus aureus (MRSA) is a cephalosporin and beta-lactam antibiotic-resistant strains. In most cases, that is spread from infected patients and infection rates are growing increasingly. Thus, accordingly, increased resistance to antibiotics is causing serious problems in the world. Therefore, there is a need to develop alternative antimicrobial drugs for the treatment of infections diseases. Methods : The antibacterial activities of Sinhyowoldosan were evaluated against 3 strains of Methicillin-resistant staphylococcus aureus(MRSA) and 1 standard Methicillin-susceptible S. aureus (MSSA) strain by using the disc diffusion method, minimal inhibitory concentrations (MICs) assay, colorimetric assay using MTT test, checkerboard dilution test and time-kill assay was performed under dark. Results : The MIC (minimum inhibitory concentration) of Sinhyowoldosan water extract against S. aureus strains ranged from 500 to 2,000 ${\mu}g/mL$, so we have confirmed it on a strong antibacterial effect. Also, the combinations of Sinhyowoldosan water extract and conventional antibiotics exhibited improved inhibition of MRSA with synergy effect. We suggest that Sinhyowoldosan water extract against MRSA have antibacterial activity, it has potential as alternatives to antibiotic agent. the combination test was used, Triton X-100 (TX) and DCCD for measurement of membrane permeability and inhibitor of ATPase. As a result, antimicrobial activity of SH is affected by the cell membrane were assessed. Conclusion : We suggest that the Sinhyowoldosan water extract lead the treatment of bacterial infection to solve the resistance and remaining side-effect problems that are the major weak points of traditional antibiotics.

Keywords

References

  1. Marraro RV, Mitchell JL. Experiences and observations with the typing of Staphylococcus aureus. J Clin Microbiol. 1975 ; 1(2) : 180-4.
  2. Sanford MD, Widmer AF, MJ Bale, Jones RN, Wenzel RP. Efficient detection and long-term persistence of the carriage of methicillin-resistant Staphylococcus aureus. Clin Infect Dis. 1994 ; 19(6) : 1123-8. https://doi.org/10.1093/clinids/19.6.1123
  3. Bycroft BW, Shute R. The molecular basis for the mode of action of beta-lactam antibiotics and mechanism of resistance. Pharm Res. 1985 ; 2(1) : 3-14. https://doi.org/10.1023/A:1016305704057
  4. Al-Habib A, Al-Saleh E, Safer AM, Afzal M. Bactericidal effect of grape seed extract on methicillin-resistant Staphylococcus aureus (MRSA). J Toxicol Sci. 2010 ; 35(3) : 357-64. https://doi.org/10.2131/jts.35.357
  5. Liu IX, Durham DG, Richards RM. Baicalin synergy with $\beta$-lactam antibiotics against methicillin-resistant Staphylococcus aureus other $\beta$-lactam-resistant strains of S. aureus. J Pharm Pharmacol. 2000 ; 52(3) : 361-6. https://doi.org/10.1211/0022357001773922
  6. Dawis MA, Isenberg HD, France KA, Jenkins SG. In vitro activity of gatifloxacin alone and incombination with cefepime, meropenem, piperacillin and gentamicin against multidrug-resistant organisms. J Antimicrob Chemother. 2003 ; 51(5) : 1203-11. https://doi.org/10.1093/jac/dkg238
  7. Huh J. NaeKyung-Pyun of Donguibogam. Yeogang. 2005 : 564
  8. Boo YM, Kwon DY, Seo BI, Choi HY, Lee JH. Chungyulyak/Clinical Traditional Herbalogy. Younglimsa. 2004 : 198, 461
  9. Lee YS, Kang OH, Choi JG, Oh YC, Chae HS, Kim JH, Park H, Sohn DH, Wang ZT, Kwon DY. Synergistic effects of the combination of galangin with gentamicin against methicillinresistant Staphylococcus aureus. J Microbiol. 2008 ; 46(3) : 283–8. https://doi.org/10.1007/s12275-008-0012-7
  10. Dzink JL, Socransky SS. Comparative in vitro activity of sanguinarine against oral microbial isolates. Antimicrob Agents Chemother. 1985 ; 27(4) : 663-5. https://doi.org/10.1128/AAC.27.4.663
  11. Kwon DY, Kang OH, Choi JG, Lee YS, Oh YC, Chae HS, Lee GH, Park PS, Kim YC, Sohn DH, Park H, Lee JH. Antibacterial effect of Dryopteris crassirhizoma against methicillin-resistant Staphylococcus aureus. Fitoterapia. 2007 ; 78(6) : 430-3. https://doi.org/10.1016/j.fitote.2007.03.026
  12. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial disk susceptibility tests. Approved standards. CLSI document M2 A7. Wayne, PA: CLSI. 2000
  13. Veljic M, Tarbuk M, Marin PD, Ciric A, Sokovic M, Marin M. Antimicrobial activity of methanol extracts of mosses from Serbia. Pharm Biol. 2008 ; 46(12) : 871-5. https://doi.org/10.1080/13880200802367502
  14. Shahverdi AR, Fakhimi A, Zarrini G, Dehghan G, Iranshahi M. Galbanic acid from Ferula szowitsiana enhanced the antibacterial activity of penicillin G and cephalexin against Staphylococcus aureus. Biol Pharm Bull. 2007 ; 30(9) : 1805-7. https://doi.org/10.1248/bpb.30.1805
  15. Noble WC, Virani Z, Cree RG. Co-transfer of vancomycin and other resistance genes from Enterococcus faecalis NCTC 12201 to Staphylococcus aureus. FEMS Microbiol Lett. 1992 ; 72(2) : 195-8.
  16. Guadalupe MN, Blanca EL, Mariano VP, Fortino SS. In vitro activity effects of combinations of cephalothin, dicloxacillin, imipenem, vancomycin and amikacin against methicillin-resistant Staphylococcus spp. strains. Ann Clin Microbiol Antimicrob. 2006 ; 5 : 25. https://doi.org/10.1186/1476-0711-5-25
  17. Nascimento AM, MG Brandao, GB Oliveira, IC Fortes, SE Chartone. Synergistic bactericidal activity of Eremanthus erythropappus oil or beta-bisabolene with ampicillin against Staphylococcus aureus. Antonie Van Leeuwenheok. 2007 ; 92(1) : 95-100. https://doi.org/10.1007/s10482-006-9139-x
  18. Linnett PE, Beechey RB. Inhibitors of the ATP synthethase system. Methods Enzymol. 1979 ; 55 : 472-518. https://doi.org/10.1016/0076-6879(79)55061-7
  19. Irvin RT, MacAlister TJ, Costerton JW. Tris(hydroxymethyl) aminomethane buffer modification of Escherichia coli outer membrane permeability. J Bacteriol. 1981 ; 145(3) : 1397-1403.
  20. Swallow CJ, Grinstein S, Rotstein OD. A vacuolar type H (+)-ATPase regulates cytoplasmic pH in murine macrophages. J Biol Chem. 1990 ; 265(13) : 7645-54.