Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Effect of Glycosaminoglycans on In vitro Fertilizing Ability and In vitro Developmental Potential of Bovine Embryos

  • Kim, Eun Young (Mirae Biotech/Jeju National University Stem Cell Research Center) ;
  • Noh, Eun Hyung (College of Applied Life Sciences, Jeju National University) ;
  • Noh, Eun Ji (College of Applied Life Sciences, Jeju National University) ;
  • Park, Min Jee (College of Applied Life Sciences, Jeju National University) ;
  • Park, Hyo Young (Mirae Biotech/Jeju National University Stem Cell Research Center) ;
  • Lee, Dong Sun (College of Applied Life Sciences, Jeju National University) ;
  • Riu, Key Zung (College of Applied Life Sciences, Jeju National University) ;
  • Park, Se Pill (College of Applied Life Sciences, Jeju National University)
  • Received : 2012.07.30
  • Accepted : 2012.10.12
  • Published : 2013.02.01
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Abstract

The glycosaminoglycans (GAGs) present in the female reproductive tract promote sperm capacitation. When bovine sperm were exposed to 10 ${\mu}g/ml$ of one of four GAGs (Chondroitin sulfate, CS; Dermatan sulfate, DS; Hyaluronic acid, HA; Heparin, HP) for 5 h, the total motility (TM), straight-line velocity (VSL), and curvilinear velocity (VCL) were higher in the HP- or HA-treated sperm, relative to control and CS- or DS-treated sperm. HP and HA treatments increased the levels of capacitated and acrosome-reacted sperm over time, compared to other treatment groups (p<0.05). In addition, sperm exposed to HP or HA for 1 h before IVF exhibited significantly improved fertilizing ability, as assessed by 2 pronucleus (PN) formation and cleavage rates at d 2. Exposure to these GAGs also enhanced in vitro embryo development rates and embryo quality, and increased the ICM and total blastocyst cell numbers at d 8 after IVF (p<0.05). A real-time PCR analysis showed that the expression levels of pluripotency (Oct 4), cell growth (Glut 5), and anti-apoptosis (Bax inhibitor) genes were significantly higher in embryos derived from HA- or HP-treated sperm than in control or other treatment groups, while pro-apoptotic gene expression (caspase-3) was significantly lower in all GAG treatment groups (p<0.05). These results demonstrated that exposure of bovine sperm to HP or HA positively correlates with in vitro fertilizing ability, in vitro embryo developmental potential, and embryonic gene expression.

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