Journal of Korean Medicine Rehabilitation (한방재활의학과학회지)

The society of Korean Medicine Rehabilitation (한방재활의학과학회)
권호 표지

Antibacterial Effect of Huanggeumjakyak-tang against Methicillin-resistant Staphylococcus aureus

황금작약탕(黃芩芍藥湯)의 MRSA에 대한 항균활성에 관한 연구

  • Kim, Esther (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Won-Kwang University) ;
  • Choi, Chonghwan (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Won-Kwang University) ;
  • Kim, Ilhyun (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Won-Kwang University) ;
  • Lee, Hail (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Won-Kwang University) ;
  • Song, Yungsun (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Won-Kwang University)
  • 김에스더 (원광대학교 한의과대학 한방재활의학과교실) ;
  • 최종환 (원광대학교 한의과대학 한방재활의학과교실) ;
  • 김일현 (원광대학교 한의과대학 한방재활의학과교실) ;
  • 이하일 (원광대학교 한의과대학 한방재활의학과교실) ;
  • 송용선 (원광대학교 한의과대학 한방재활의학과교실)
  • Published : 2013.07.31
⟨ Previous Next ⟩

Abstract

Objectives The aim of this study was to investigate antimicrobial activity of Huanggeumjakyak- tang water extract against MRSA. Methods The antibacterial activities of Huanggeumjakyak-tang were evaluated against 3 strains of Methicillin-resistant Staphylococcus aureus (MRSA) and 1 standard Methicillinsusceptible S. aureus (MSSA) strain by using the disc diffusion method, minimal inhibitory concentrations (MICs) assay, colorimetric assay using MTT test, checkerboard dilution test and time-kill assay was performed under dark. Results The MIC (minimum inhibitory concentration) of Huanggeumjakyak-tang water extract against S. aureus strains ranged from 1,000 to $2,000{\mu}g/ml$. So we confirmed that it has a strong antibacterial effect. Also the combinations of Huanggeumjakyak-tang water extract and conventional antibiotics exhibited improved inhibition of MRSA with synergy effect. Conclusions The results obtained in this study suggest that Huanggeumjakyak-tang water extract showed antibacterial effect against MRSA, and it also showed reducing effect on the side-effect problems that are the major weak points of traditional antibiotics.

Keywords

References

  1. Cosgrove SE. The relationship between antimicrobial resistance and patient outcomes: mortality, length of hospital stay, and health care costs. Clin Infect Dis. 2006;42(2):S82-9. https://doi.org/10.1086/499406
  2. Sandora TJ, Goldmann DA. Preventing lethal hospital outbreaks of antibiotic-resistant bacteria. N Engl J Med. 2012;367(23):2168-70. https://doi.org/10.1056/NEJMp1212370
  3. 최일, 조재용, 임상철. Staphylococcus aureus에 대한 한약재의 항균활성. 한국자원식물학회지. 2006;19(4):491-6.
  4. Ghosh A, Das BK, Roy A, Mandal B, Chandra G. Antibacterial activity of some medicinal plant extracts. Journal of Natural Medicines. 2008;62(2):259-62. https://doi.org/10.1007/s11418-007-0216-x
  5. Aqil F, Khan MS, Owais M, Ahmad I. Effect of certain bioactive plant extracts on clinical isolates of beta-lactamase producing methicillin resistant Staphylococcus aureus. J Basic Microbiol. 2005;45(2):106-14. https://doi.org/10.1002/jobm.200410355
  6. 張仲景. 景岳全書. 臺北:臺聯國風出版社. 1974:201.
  7. 許浚. 東醫寶鑑. 서울:법인문화사. 1999:450.
  8. 申載鏞. 方藥合編解說. 서울:成輔社. 2000:249-50.
  9. 尹用甲. 東醫方劑와 處方解說. 서울:醫聖堂. 1998:62-3.
  10. 양재헌, 김영일. 바이칼린을 함유한 황금 엑스 유제의 제조 및 항균효과. 약제학회지. 1998;28(3):159-64.
  11. 吳春根. 黃芩芍藥湯 煎湯液이 血漿 Cortisol 및 鎭痛에 미치는 影響. 대한한방안이비인후피부과학회지. 1990;3(1):41-57.
  12. 이실, 최종백. 黃芩芍藥湯이 腹痛 下痢 및 鎭痛效果에 미치는 影響에 關한 硏究. 동서의학. 1988;13(1):20-33.
  13. Lee YS, Kang OH, Choi JG, Oh YC, Chae HS, Kim JH, Park H, Sohn DH, Wang ZT, Kwon DY. Synergistic effects of the combination of galangin with gentamicin against methicillin-resistant Staphylococcus aureus. J Microbiol. 2008;46(3):283-8. https://doi.org/10.1007/s12275-008-0012-7
  14. Kwon DY, Kang OH, Choi JG, Lee YS, Oh YC, Chae HS, Lee GH, Park PS, Kim YC, Sohn DH, Park H, Lee JH. Antibacterial effect of Dryopteris crassirhizoma against methicillin-resistant Staphylococcus aureus. Fitoterapia. 2007;78(6):430-3. https://doi.org/10.1016/j.fitote.2007.03.026
  15. Bala M, Ray K, Gupta SM. Comparison of disc diffusion results with minimum inhibitory concentration (MIC) values for antimicrobial susceptibility testing of Neisseria gonorrhoeae. Indian J Med Res. 2005;122(1):48-51.
  16. CLSI. Performance standards for antimicrobial disk susceptibility tests. Approved standards. CLSI document M2 A7. Wayne, PA, 2001.
  17. Shahverdi AR, Fakhimi A, Zarrini G, Dehghan G, Iranshahi M. Galbanic acid from Ferula szowitsiana enhanced the antibacterial activity of penicillin G and cephalexin against Staphylococcus aureus. Biol Pharm Bull. 2007;30(9):1805-7. https://doi.org/10.1248/bpb.30.1805
  18. Mazumdar K, Dutta NK, Kumar KA, Dastidar SG. In vitro and in vivo synergism between tetracycline and the cardiovascular agent oxyfedrine HCl against common bacterial strains. Biol Pharm Bull. 2005;28(4):713-7. https://doi.org/10.1248/bpb.28.713
  19. Timurkaynak F, Can F, Azap OK, Demirbilek M, Arslan H, Karaman SO. In vitro activities of non-traditional antimicrobials alone or in combination against multidrug-resistant strains of Pseudomonas aeruginosa and Acinetobacter baumannii isolated from intensive care units. Int J Antimicrob Agents. 2006;27(3):224-8. https://doi.org/10.1016/j.ijantimicag.2005.10.012
  20. Nascimento AM, Brandao MG, Oliveira GB, Fortes IC, Chartone-Souza E. Synergistic bactericidal activity of Eremanthus erythropappus oil or beta-bisabolene with ampicillin against Staphylococcus aureus. Antonie Van Leeuwenheok. 2007;92(1):95-100. https://doi.org/10.1007/s10482-006-9139-x
  21. Choi JG, Kang OH, Obiang-Obounou B, Lee YS, Chae HS, Oh YC, Sohn DH, Park H, Choi HG, Kim SG, Shin DW, Kwon DY. Antibacterial activity of Ecklonia cava against methicillin-resistant Staphylococcus aureus and Salmonella spp. Foodborne Pathog Dis. 2010;7(4):435-41. https://doi.org/10.1089/fpd.2009.0434
  22. Jung HJ, Lee DG. Synergistic antibacterial effect between silybin and N,N'-dicyclohexylcarbodiimide in clinical Pseudomonas aeruginosa isolates. J Microbiol. 2008;46(4):462-7. https://doi.org/10.1007/s12275-008-0138-7
  23. Stuart BL. The antibiotic paradox: how miracle drugs are destroying the miracle. New York:Plenum publishing Co., 1992:114-29.
  24. Choi OK, Noh YC, Hwang SY. Antimicrobial activity of grape fruit seed extracts and polylycine mixture against food-borne pathogens. Korean J Dietary Culture. 2000;15:9-15.
  25. Kang MW, and Kim YR. Infection of methicillin resistant Staphylococcus aureus. J Korean Soc Chemother. 1993;11:17-26.
  26. Ha DJ, Kim YC, Kim YJ. Management of infection for methicillin resistant Staphylococcus aureus at an orthopaedic surgery department. J Korean Orthop. 2003;38:34-8.
  27. Ahn JY, Kim WB, Lee DW, Lee K, Choi SH, Kim IS, Seo CH. A study of the mecI, mecA and femA genes of methicillin-resistant Staphylococci. Korean J Clin Pathol. 1999;19:62-9.
  28. Morell EA, Balkin DM. Methicillin-resistant Staphylococcus aureus: a pervasive pathogen highlights the need for new antimicrobial development. Yale J Biol Med. 2010;83:223-33.
  29. Braunwald E et al. HARRISON'S 내과학. 서울: MIP. 2003:909-19.
  30. Lee JS, Park O, Woo H J, Jung HJ, Kim WJ, Kim MJ, Park SC. A longitudinal molecular epidemiologic study of methicillin-resistant Staphylococcus aureus. isolates from a University Hospital. Infection. 2001;33:32-40.
  31. 전국한의과대학 본초학공동교재 편찬위원회. 本草學. 서울:영림사. 2000:178-9, 581-3, 540-1.
  32. 具本泓. 漢方處方解說. 大邱:東洋綜合通信敎育院出版. 1982:64-5.
  33. 李尙仁. 天眞處方解說. 서울:成輔社. 1987:327.
  34. Lee CK, Seo JJ. Antimicrobial activity of Whangkumtang extract and Scutellariae Radix extract on the Food-Borne pathogens. J Kor Soc Food Sci Nutr. 2005:34(10):1606-10. https://doi.org/10.3746/jkfn.2005.34.10.1606
  35. 부경환, 이도성, 우진규, 고성희, 정은형, 홍관천, 류기정, 이동선. 작약뿌리 추출물의 항균 및 항바이러스 활성. 한국응용생명화학회지. 2011;4(1):132-5.
  36. 강태규. 감초의 항균활성 및 세포독성에 미치는 영향. 상지대학교. 석사학위논문. 2008.