Journal of Korean Medicine Rehabilitation (한방재활의학과학회지)

The society of Korean Medicine Rehabilitation (한방재활의학과학회)
권호 표지

Anti-inflammatory Effect of Mugi-hwan Water Extract in RAW 264.7 Cells

무기환(戊己丸)의 RAW 264.7 세포에 대한 항염증작용 연구

  • Kim, Ilhyun (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Won-Kwang University) ;
  • Choi, Chonghwan (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Won-Kwang University) ;
  • Lee, Sewon (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Won-Kwang University) ;
  • Song, Yungsun (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Won-Kwang University)
  • 김일현 (원광대학교 한의과대학 한방재활의학과교실) ;
  • 최종환 (원광대학교 한의과대학 한방재활의학과교실) ;
  • 이세원 (원광대학교 한의과대학 한방재활의학과교실) ;
  • 송용선 (원광대학교 한의과대학 한방재활의학과교실)
  • Published : 2013.07.31
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Abstract

Objectives The aim of this study was to investigate anti-inflammatory activity of Mugi-hwan (MGH) Water Extract. Methods Cells were treated with 2 ug/ml of LPS 1 hour prior to the addition of MGH. Cell viability was measured by MTS assay. The production of NO was determined by reacting cultured medium with Griess reagent. The expression of COX-2, iNOS and MAPKs was investigated by Western blot, RT-PCR. The content of level of cytokines ($PGE_2$, IL-6, in media from LPS-stimulated Raw 264.7 cells was analyed by ELISA kit. Results MGH inhibited the production of NO, $PGE_2$, IL-6 as well as the expressions of iNOS, COX-2 in the murine macrophage, RAW 264.7 cells. MGH also had suppression effects of LPS induced MAPKs activation. Conclusions These results suggest that MGH has an anti-inflammatory therapeutic potential, which may result from inhibition of MAPK phosphorylation, thereby decreasing the expression of pro-inflammatory genes.

Keywords

References

  1. Morson BC. Pathology of inflammatory bowel disease. Gastroenterol Jpn. 1980;15(2):184-7.
  2. Cline MJ. Leukocyte function in inflammation: the ingestion, killing, and digestion of microorganisms. Ser Haematol. 1970;3(2):3-16.
  3. Bosca L, Zeini M, Traves PG, Hortelano S. Nitric oxide and cell viability in inflammatory cells: a role for NO in macrophage function and fate. Toxicology. 2005;208(2):249-58. https://doi.org/10.1016/j.tox.2004.11.035
  4. Nathan C. Nitric oxide as a secretory product of mammalian cells. FASEB J. 1992;6(12):3051-64.
  5. Turini ME, DuBois RN. Cyclooxygenase-2: a therapeutic target. Annu Rev Med. 2002;53:35-57. https://doi.org/10.1146/annurev.med.53.082901.103952
  6. Wei W, Li XY, Zhang HQ, Wu SG. Antiinflammatory and immuno pharma cology. 1st ed. Beijing:Renmin weisheng chubanshe. 2004:10-7.
  7. Andreakos E, Foxwell B, Feldmann M. Is targeting Toll-like receptors and their signaling pathway a useful therapeutic approach to modulating cytokine-driven inflammation. Immunol Rev. 2004;202:250-65. https://doi.org/10.1111/j.0105-2896.2004.00202.x
  8. 宋太平惠民和劑局. 太平惠民和劑局方. 中國中醫藥出版社. 1996:158.
  9. 許俊. 東醫寶鑑. 서울:동의보감출판사. 2007:406.
  10. Bae EA, Han MJ, KIM NJ, Kim DH. Anti-Helicobacter pylori activity of herbal medicines. Biol Pharm Bull. 1998;21(9):990-2. https://doi.org/10.1248/bpb.21.990
  11. 정효원, 박용기. 황련 추출물의 분획화 및 BV2 microglial cells에서 LPS에 의해 유도되는 nitric oxide 생성억제효과 검정. 대한본초학회지. 2007;22(2):73-8.
  12. Kuo CL, Chi CW, Liu TY. The anti-inflammatory potential of berberine in vitro and in vivo. Cancer Lett. 20. 2004;203(2):127-37. https://doi.org/10.1016/j.canlet.2003.09.002
  13. Takada Y, Kobayashi Y, Aggarwal BB. Evodiamine abolishes constitutive and inducible NF-kappaB activation by inhibiting IkappaBalpha kinase activation, thereby suppressing NF-kappaB-regulated antiapoptotic and metastatic gene expression, up-regulating apoptosis, and inhibiting invasion. J Biol Chem. 29. 2005;280(17):17203-12.
  14. Kang SS, Kim JS, Kim EM, Yun-Choi HS. Platelet anti-aggregation of Paeony root. Kor J Pharmacogn. 1991;22: 215-8.
  15. Ro HS, Ko WK, Yang HO, Park KK, Cho YH, Park HS. Effect of several solvent extracts from Paeoniae radix on experimental hyperlipidemia in rats. J Kor Parm Sci. 1997;27:145-51.
  16. Kim YE, Lee YC, Kim HK, Kim CJ. Antioxidative effect of ethanol fraction for several Korean medicinal plant hot water extracts. Korean J Food & Nutr. 1997;10:141-4.
  17. Goto H, Shimada Y, Akechi Y, Kohta K, Hattori M, Terasawa K. Endothelum-dependent vasodilator effect of extract prepared from the root of Paeonia lactiflora on isolated rat aorta. Planta Med. 1996;62:436-9. https://doi.org/10.1055/s-2006-957934
  18. 신민교. 임상본초학. 서울:영림사. 2006:242,307,403.
  19. Medzhitov R. Origin and physiological roles of inflammation. Nature. 2008;454:428-35. https://doi.org/10.1038/nature07201
  20. Zamora R, Vodovotz Y, Billiar TR. Inducible nitric oxide synthase and inflammatory diseases. Mol Med. 2000;6:347-73.
  21. Jew SS, Bae ON, Chung JH. Anti-inflammatory effects of asiaticoside on inducible nitric oxide synthase and cyclooxygenase-2 in RAW 264.7 cell line. J Toxicol Pub Health. 2003;19:33-7.
  22. Ljung T, Lundberg S, Varsanyi M, Johansson C, Schmidt PT, Herulf M, Lundberg JO, Hellstrom PM. Rectal nitric oxide as biomaker in the treatment of inflammatory bowel disease: responders versus non-responders. World J Gastroenterol. 2006;12:3386-92. https://doi.org/10.3748/wjg.v12.i21.3386
  23. Lee ES, Ju HK, Moon TC, Lee E, Jahng Y, Lee SH, Son JK, Baek SH, Chang HW. Inhibition of nitric oxide and tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) production by propenone compound through blockade of nuclear factor (NF)-${\kappa}B$ activation in cultured murine macrophages. Biol Pharm Bull. 2004;27:617-20. https://doi.org/10.1248/bpb.27.617
  24. Bosca L, Zeini M, Traves PG, Hortelano S. Nitric oxide and cell viability in inflammatory cells: a role for NO in macrophage function and fate. Toxicology. 2005;208(2):249-58. https://doi.org/10.1016/j.tox.2004.11.035
  25. Nathan C. Nitric oxide as a secretory product of mammalian cells. FASEB J. 1992;6(12):3051-64.
  26. Turini ME, DuBois RN. Cyclooxygenase-2: a therapeutic target. Annu Rev Med. 2002;53:35-57. https://doi.org/10.1146/annurev.med.53.082901.103952
  27. Iontcheva I, Amar S, Zawawi KH, Kantarci A, Van Dyke TE. Role for moesin in lipopolysaccharidestimulated signal transduction. Infect Immun. 2004;72:2312-20. https://doi.org/10.1128/IAI.72.4.2312-2320.2004
  28. Gomez PF, Pillinger MH, Attur M, Marjanovic N, Dave M, Park J, Bingham CO, Al-Mussawir H, Abramson SB. Resolution of inflammation: prostaglandin E2 dissociates nuclear trafficking of individual NFkappaB subunits (p65, p50) in stimulated rheumatoid synovial fibroblasts. J Immunol. 2005;175:6924-30. https://doi.org/10.4049/jimmunol.175.10.6924
  29. Kavya R, Saluja R, Singh S, Dikshit M. Nitric oxide synthase regulation and diversity: implications in Parkinson's disease. Nitric Oxide. 2006;15:280-94. https://doi.org/10.1016/j.niox.2006.07.003
  30. Weigert A, Brune B. Nitric oxide, apoptosis and macrophage polarization during tumor progression. Nitric Oxide. 2008;19:95-102. https://doi.org/10.1016/j.niox.2008.04.021
  31. Lee, TH, Kwak HB, Kim HH, Lee ZH, Chung DK, Baek NI, Kim J. Methanol extracts of Stewartia koreana inhibit cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) gene expression by blocking NF-kappa B transactivation in LPS-activated RAW 264.7 cells. Mol Cells. 2007;23:398-404.
  32. Kim EO, Min KJ, Kwon TK, Um BH, Moreau RA, Choi SW. Anti-inflammatory activity of hydroxycinnamic acid derivatives isolated from corn bran in lipopolysaccharidestimulated Raw 264.7 macrophages. Food Chem Toxicol. 2012;50:1309-16. https://doi.org/10.1016/j.fct.2012.02.011
  33. Delgado AV, McManus AT, Chambers JP. Production of tumor necrosis factor-alpha, interleukin 1-beta, interleukin 2, and interleukin 6 by rat leukocyte subpopulations after exposure to substance P. Neuropeptides 2003;37:355-61. https://doi.org/10.1016/j.npep.2003.09.005
  34. Feldmann M, Brennan FM, Maini RN. Role of cytokines in rheumatoid arthritis. Annu Rev Immunol. 1996;14:397-440. https://doi.org/10.1146/annurev.immunol.14.1.397
  35. Ahn KS, Noh EJ, Zhao HL, Jung SH, Kang SM, Kim YS. Inhibition of inducible nitric oxide synthase and cyclooxygenase II by Platycodon grandiflorum saponins via suppression of nuclear factor-kappaB activation in HaCaT cells. Life Sci. 2005;76:2315-28. https://doi.org/10.1016/j.lfs.2004.10.042
  36. Chen C, Chen YH, Lin WW. Involvement of p38 mitogen-activated protein kinase in lipopolysaccharide-induced iNOS and COX-2 expression in J774 macrophage. Immunology. 1999;97:124-9. https://doi.org/10.1046/j.1365-2567.1999.00747.x
  37. Chen Y, Yang L, Lee TJ. Oroxylin A inhibition of lipopolysaccharide-induced iNOS and COX-2 gene expression via suppression of nuclear factor-kappaB activation. Biochem Pharmacol. 2000;59:1445-57. https://doi.org/10.1016/S0006-2952(00)00255-0