환경생물 (Korean Journal of Environmental Biology)

  • 제31권4호
  • /
  • Pages.486-492
  • /
  • 2013
  • /
  • 1226-9999(pISSN)
  • /
  • 2287-7851(eISSN)
한국환경생물학회 (Korean Society of Environmental Biology)
권호 표지
DOI QR코드

DOI QR Code

Aeromonas veronii의 실험적 감염에 의한 양식 메기, Silurus asotus의 병리학적 변화

Pathological Changes in Cultured Korean Catfish (Silurus asotus) Artficially Infected with Aeromonas veronii

  • 김진도 (국립수산과학원 전략양식연구소 병리연구과) ;
  • 도정완 (국립수산과학원 전략양식연구소 병리연구과) ;
  • 최혜승 (국립수산과학원 전략양식연구소 병리연구과) ;
  • 서정수 (국립수산과학원 전략양식연구소 병리연구과) ;
  • 정승희 (국립수산과학원 전략양식연구소 병리연구과) ;
  • 조혜인 (국립수산과학원 전략양식연구소 병리연구과) ;
  • 박명애 (국립수산과학원 기반연구부 수산생물방역과) ;
  • 이남실 (국립수산과학원 전략연구단) ;
  • 박성우 (군산대학교 해양과학대학 수산생명의학과)
  • Kim, Jin-Do (Pathology Division, Aquaculture Research Institute, National Fisheries Research & Development Institute) ;
  • Do, Jeong-Wan (Pathology Division, Aquaculture Research Institute, National Fisheries Research & Development Institute) ;
  • Choi, Hye-Sung (Pathology Division, Aquaculture Research Institute, National Fisheries Research & Development Institute) ;
  • Seo, Jung-Soo (Pathology Division, Aquaculture Research Institute, National Fisheries Research & Development Institute) ;
  • Jung, Sung-Hee (Pathology Division, Aquaculture Research Institute, National Fisheries Research & Development Institute) ;
  • Jo, Hyae-In (Pathology Division, Aquaculture Research Institute, National Fisheries Research & Development Institute) ;
  • Park, Myung-Ae (Aquatic Life Disease Control Division, NFRDI) ;
  • Lee, Nam-Sil (New Strategy Research Center, NFRDI) ;
  • Park, Sung-Woo (Department of Aquatic Life Medicine, Kunsan National University)
  • 투고 : 2013.11.01
  • 심사 : 2013.11.12
  • 발행 : 2013.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

최근 국내의 양식 메기에 표피 박리와 근육 괴사를 특징으로 하는 새로운 질병이 발생하였다. 그 폐사율은 낮으나 질병으로 인한 상품가치의 저하로 경제적 손실이 크다. 병어로부터 원인균을 분리하고 자연감염어와 인위감염어의 병리학적 변화를 관찰하였다. 원인균은 Aeromonas veronii로 동정되었으며, 원인균을 건강어에 인위감염시켜 폐사 및 증상의 발현을 매일 관찰하였다. 인위감염된 어류의 증상은 자연감염어와 유사하였으며 원인균을 감염시킨 후 7일 이내에 모두 폐사하였다. 병어의 조직표본을 제작하여 관찰한 결과, 자연감염어의 간, 비장, 신장의 울혈 및 간세포 변성과 비장 협조직의 초자적변성이 관찰되었으며, 특히 심장에서 염증성 변성과 세균응집체가 관찰되었다. 소화관에서의 울혈과 점막고 유층의 섬유화 현상도 특징적이었다. 자연감염어에 비해 약한 경향이지만 인위감염어의 조직학적 변화도 관찰되었으며, 소화관에서의 조직학적 변성은 관찰되지 않았다.

Recently, a new disease showing symptoms such as epidermal exfoliation and muscular necrosis occurred in cultured Korean catfish. Although the mortality of fishes was low but the economic damages owing to loss of commercial value were severe. The authors isolated the causative agent from diseased fish and observed pathological changes both in naturally and artificially infected fish. The causative bacteria was identified as Aeromonas veronii. Subsequently we observed the daily death and pathological symptoms of artificially infected fish with Aeromonas veronii. Symptoms of artificially infected fish were similar to those of naturally infected fish and all fish died within 7 days after infection. Histopathological changes on the naturally infected fish revealed severe congestion and necrotic degeneration in the liver, spleen and kidney. Some bacterial aggregates with inflammatory degeneration were observed in the heart, and congestion and fibrosis in the lamina propria of digestive tube were predominant. In artificially infected fish, skin erosion and necrotic degeneration of muscle tissue around injected region were particularly manifested. Degeneration of hepatocytes in liver and hyalic degeneration around ellipsoids in spleen were partially observed. However, there were no predominant signs in digestive tube in artificially infected fish.

키워드

참고문헌

  1. Abbott SL, WKW Cheung and JM Janda. 2003. The Genus Aeromonas: Biochemical Characteristics, Atypical Reaction, and Phenotypic Indentification Schemes. J. Clin. Microbiol. 41:2348-2357. https://doi.org/10.1128/JCM.41.6.2348-2357.2003
  2. Carnahan AM, S Berram and SW Joseph. 1991. Aerokey II: A Flexible Key for Idetifying Clinical Aeromonas Species. J. Clinic. Microbiol. 29:2843-2849.
  3. Gonzalez-Serrano CJ, JA Santos, ML Garcia-Lopez and A Otero. 2002. Virulence markers in Aeromonas hydrophila and Aeromonas veronii biovar sobria isolates from freshwater fish and from a diarrhoea case. J. Appl. Microbiol. 93:414-419. https://doi.org/10.1046/j.1365-2672.2002.01705.x
  4. Janda JM and SL Abbott. 2010. The Genus Aeromonas: Taxonomy, Pathogenicity, and infection. Clin. Microbiol. Rev. 23:35-73. https://doi.org/10.1128/CMR.00039-09
  5. Joseph SW and A Carnahan. 1994. The isolation, identification, and systematics of the motile Aeromonas species. Ann. Rev. Fish Dis. 4:315-343. https://doi.org/10.1016/0959-8030(94)90033-7
  6. Kang D-L and HK Lee. 2000. Analysis of 16S-23S rRNA Intergenic Spacer Regions of Aeromonas veronii biogroup sobria and Aeromonas caviae. Kor. J. Microbiol. 36:173-180.
  7. Kim J-H, S-W Park, C-I Park, M-J Oh, S-J Jung and M-D Hur. 2006. Infection and parasitic diseases of fish and shellfish. Life Sci. Publish. Co.
  8. Krzyminska S, A Kaznowski and H Spychala. 2006. Purification and Characterization of Cytolytic Toxins Produced by Aeromonasa hydrophila and Aeromonas veronii Biotype Sobria Strains. Poli. J. Microbiol. 55:37-42.
  9. Lamy B, F Laurent, I Verdier, JW Decousser, E Lecaillon, H Marchandin, F Roger, S Tiguad, H de Montclos, the colBVH study Group and A Kodjo. 2010. Accuracy of 6 commercial systems for identifying clinical Aeromonas isolates. Diagn Microbiol. Infect. Dis. 67:9-14. https://doi.org/10.1016/j.diagmicrobio.2009.12.012
  10. Lee H-K, T-Y Lee and B-S Kim. 1993. Histopathogenic Characteristics of Haemorhagic Ulcer in Cultivated Sankehead Channa argus Artificially Infected with Aeromonas veronii. Kor. J. Microbiol. 31:113-122.
  11. Lee HK. 1992. Pathology of Ulcerous Disease in Cultivated Snakehead, Channa argus. Kor. J. Microbiol. 30:164-170.
  12. Nawaz M, SA Khan, AA Khan, K Sung, Q Tran, K Kerdahi and R Steele. 2010. Detection and characterization of virulence genes and integrons in Aeromonas veronii isolated from catfish. Food Microbiol. 27:327-331. https://doi.org/10.1016/j.fm.2009.11.007
  13. Orozova P, M Baker, DA Austin and B Austin. 2009. Identification and Pathogenicity to rainbow trout, Oncorhynchus mykiss (Walbaum) of some aeromonads. J. Fish Dis. 32:865-872. https://doi.org/10.1111/j.1365-2761.2009.01065.x
  14. Park S-W and M-J Oh. 2011. The Pathology of Aquatic Life. Bioscience.
  15. Rahman M, P Colque-Navarro, I Kuhn, G Huys, J Swings and R Mollby. 2002. Idetification and characterization of pathogenic Aeromonas veronii biovar sobria associated with epizootic ulcerative syndrome in fish in bangladesh. Appl. Environ. Micronbiol. 68:650-655. https://doi.org/10.1128/AEM.68.2.650-655.2002
  16. Sreedharan K, R Philip and IS Singh. 2011. Isolation and characterization of virulent Aeromonas veronii from ascitic fluid of oscar Astronotus ocellatus showing signs of infectious dropsy. Dis. Aquat. Org. 94:29-39. https://doi.org/10.3354/dao02304
  17. Sugita H, K Tanaka, M Yoshinami and Y Deguchi. 1995. Distribution of Aeromonas Species in the Intestinal Tracts of River Fish. Appl. Environ. Microbiol. 61:4128-4130.
  18. Toranzo AE, Y Santos, TP Nieto and JL Barja. 1986. Evaluation of different assay systems for identification environmental Aeromonas strains. Appl. Environ. Microbiol. 51:652-656.
  19. Yi S-W, M-J You, H-B Lee and G-W Shin. 2012. A case of Aeromonas veronii infection in Israeli carp (Cyprinus carpio): phylogenetic analysis and antimicrobial resistance. Kor. J. Vet. Serv. 35:239-243. https://doi.org/10.7853/kjvs.2012.35.3.239
  20. Yu JH, JJ Han, HJ Kim, SG Kang and SW Park. 2010. First report of Aeromonas veronii infection in farmed Israeli carp Cyprinus carpio in Kor. J. Fish. Pathol. 23:165-176.