DOI QR코드

DOI QR Code

Reduction in antigenesity of whey protein by alcalase

Alcalase에 의한 유청단백질 가수분해물의 항원성 저감 효과

  • Yu, Jae-Min (Lab. of Milk Food Biochemistry and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Renchinkhand, Renchinkhand (Lab. of Milk Food Biochemistry and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Bae, Hyoung Churl (Lab. of Milk Food Biochemistry and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Jeong, Seok Geun (National Institute of Animal Svience, Rural Development Administration) ;
  • Paik, Seung-Hee (Div. of Food Service Industry, Cheonan Yonam College) ;
  • Nam, Myoung Soo (Lab. of Milk Food Biochemistry and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University)
  • 유재민 (충남대학교 동물바이오시스템과학과) ;
  • 렌친핸드 (충남대학교 동물바이오시스템과학과) ;
  • ;
  • 정석근 (농촌진흥청 축산과학원 축산물이용과) ;
  • 백승희 (천안연암대학 외식산업계열) ;
  • 남명수 (충남대학교 동물바이오시스템과학과)
  • Received : 2013.11.25
  • Accepted : 2013.12.16
  • Published : 2013.12.31

Abstract

The aim of this study was to produce enzymatic hydrolysis of ${\alpha}$-LA, ${\beta}$-LG and BSA with alcalase for the possible application of hypoallergenic foods toward cow's milk allergenic infant. The molecular weights of most of the peptides in hydrolysates from ${\alpha}$-LA, ${\beta}$-LG and BSA by alcalase were below 3,000 dalton. Antigenesity of ${\alpha}$-LA, ${\beta}$-LG and BSA hydrolysates to rabbit anti-${\alpha}$-LA antiserum, ${\beta}$-LG antiserum and BSA antiserum were remarkably decreased by more than $10^{-3}$ at 20% inhibitionrate. Antigenesity of polyvalent antigenic peptide in ${\alpha}$-LA, ${\beta}$-LG and BSA hydrolysates to specific rabbit anti-${\alpha}$-LA antiserum, ${\beta}$-LG antiserum and BSA antiserum was determined by PCS test using guina-pig. Hydrolysates of ${\alpha}$-LA, ${\beta}$-LG and BSA with less than 3,000 dalton did not show polyvalent antigenic reaction against rabbit antiserum. Hydrolysates of ${\alpha}$-LA, ${\beta}$-LG and BSA could be a source for the manufacturing of hypoallergenic food.

Keywords

References

  1. Burks AW, Williams LW, Castell HB. 1990. Antibody response to milk proteins in patients with milk-protein intorancedocumented by challenge. Journal of the Allergy Clinical Immunology 85:921-927. https://doi.org/10.1016/0091-6749(90)90078-I
  2. Chicon R, Lopez-Fandino R, Alonso E, Belloque J. 2008. Proteolytic pattern, antigenicity, and serum immunoglobulin E binding of ${\beta}$-lactoglobulin hydrolysates obtain by pepsin and high-pressure treatments. Journal of Dairy Science 91:928-938. https://doi.org/10.3168/jds.2007-0657
  3. Dalgalarrondo M, Dufour E, Chobert JM, Bertrand-Harb C, Haertle T. 1995. Proteolysis of ${\beta}$-casein by pepsin in ethanolic media. International Dairy Journal 5:1-14. https://doi.org/10.1016/0958-6946(94)P1595-5
  4. Friedman NJ, Zeiger RS. 1996. Risk factors and prevention of allergy. In Bierman CW, Pearlman DS, Shapiro GG, Busse WW(Eds) Allergy, asthma and immunology from infants to childhood. 3rd ed., Philadephia, Saunders Co., pp 282-296.
  5. Kim SB, Seo IS, Khan MA, Ki KS, Nam MS, Kim HS. 2007. Separationof iron-binding protein whey through enzymatic hydrolysis. International Dairy Journal 17:625-631. https://doi.org/10.1016/j.idairyj.2006.09.001
  6. Kim SB, Ku MJ, Cho WM, Ki KS, Kim HS, Nam MS. 2010. production of iron-binding peptides from colostral whey by enzymatic hydrolysis. Korean Journal for Food Science of Animal Resources 30:923-929. [in Korean] https://doi.org/10.5851/kosfa.2010.30.6.923
  7. Marshall K, 2004. Therapeutic applications of whey protein. Alternative Medicine Review. 9:136-156.
  8. Reddy IM, Kella NK, Kinsella JE. 1988. Structural and confermational basis of the resistance of ${\beta}$-lactoglobulin to peptic and chymotryptic digestion. Journal of Agriculture and Food Chemistry 36:737-741 https://doi.org/10.1021/jf00082a015
  9. Sampson HA. 1997. Eczema and food hypersensitivity. In Metcalfe DD, Sampson HA, Simon RA(Eds) : Food allergy. 2nd ed., Messachusetts, Blackwell Science Co., pp 193-209.
  10. Saarinen KM, Pelkonen AS, Makela MJ, Savilahti E. 2005. Clinical course and prognosis of cow's milk allergy are dependent on milk-specific IgE ststus. Journal of the Allergy Clinical Immunology 116:869-875. https://doi.org/10.1016/j.jaci.2005.06.018
  11. Sicherer SH, Sampson HA, 2010. Food Allergy. Journal of the Allergy Clinical Immunology 125:S116-S125. https://doi.org/10.1016/j.jaci.2009.08.028
  12. Wal JM. 1998. Cow's milk allerges. Allergy 53:1013-1022. https://doi.org/10.1111/j.1398-9995.1998.tb03811.x