Journal of Korean Society of Forest Science (한국산림과학회지)

Korean Society of Forest Science (한국산림과학회)
권호 표지

Isolation and Characterization of a Formate Dehydrogenase cDNA in Poplar (Populus alba ${\times}$ P. glandulosa)

현사시나무에서 Formate Dehydrogenase cDNA의 분리와 특성 구명

  • Bae, Eun-Kyung (Division of Forest Biotechnology, Korea Forest Research Institute) ;
  • Lee, Hyoshin (Division of Forest Biotechnology, Korea Forest Research Institute) ;
  • Lee, Jae-Soon (Division of Forest Biotechnology, Korea Forest Research Institute) ;
  • Choi, Young-Im (Division of Forest Biotechnology, Korea Forest Research Institute) ;
  • Yoon, Seo-Kyung (Division of Forest Biotechnology, Korea Forest Research Institute) ;
  • Eo, Soo Hyung (Department of Forest Resources, Kongju National University)
  • 배은경 (국립산림과학원 산림생명공학과) ;
  • 이효신 (국립산림과학원 산림생명공학과) ;
  • 이재순 (국립산림과학원 산림생명공학과) ;
  • 최영임 (국립산림과학원 산림생명공학과) ;
  • 윤서경 (국립산림과학원 산림생명공학과) ;
  • 어수형 (공주대학교 산림자원학과)
  • Published : 2013.09.30
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Abstract

Formate dehydrogenase (FDH), catalyzing the oxidation of the formate ion to carbon dioxide, is known as the stress protein in response to drought, low temperature and pathogen infection. To study the functions of FDH in poplar (Populus alba ${\times}$ P. glandulosa), we isolated a FDH cDNA (PagFDH1) and examined its expressional characteristics. The PagFDH1 is 1,499 base pairs long and encodes a putative 388 amino acid protein with an expected molecular mass of 42.5 kDa. The PagFDH1 protein has N-terminal mitochondria signal peptide and $NAD^+$ binding domain. Southern blot analysis indicated that a single copy of the PagFDH1 is present in the poplar genome. PagFDH1 is expressed highly in the suspension cells (especially in the lag and early exponential phases) and moderately in roots, flowers and leaves. ABA-mediated enhanced expression of PagFDH1 in response to drought and salt stress treatments indicates that the gene product could play an important role in the development of stress resistant trees.

Formate dehydrogenase(FDH)는 포름산이온을 이산화탄소로 산화하는 반응을 촉매하는 효소로서, 건조와 저온 그리고 병원균 감염 등에 반응하는 스트레스 단백질로 알려져 있다. 본 연구에서는 현사시나무에서 FDH의 cDNA를 분리하여 구조와 발현 특성 등을 조사하였다. 현사시나무의 FDH cDNA(PagFDH1)는 1,499개의 염기쌍으로 이루어져 있으며, 388개의 아미노산으로 구성되는 예상 분자량 42.5 kDa의 단백질을 암호화한다. PagFDH1 단백질은 미토콘드리아 신호펩티드와 $NAD^+$ 결합부위를 가지고 있다. PagFDH1은 현사시나무의 염색체에 1 copy가 존재하며, 배양세포에서 가장 높게 발현되고 뿌리와 꽃 그리고 잎에서도 발현되었다. 현탁배양세포의 생장주기에서 유도기와 초기 지수생장기에 높게 발현하였다. PagFDH1은 건조와 염 스트레스에 반응하여 ABA를 경유한 신호전달경로에 의해 발현이 유도되는 것으로 나타났다. 본 연구결과는 FDH 유전자의 도입과 발현조절을 통한 환경 스트레스 저항성나무의 개발에 도움을 줄 것으로 생각된다.

Keywords

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